22 results about "Sex Determination Process" patented technology

The invention relates to a sex-related SNP (Single Nucleotide Polymorphism) molecular marker of lateolabrax japonicus as well as a detection reagent and application thereof, and belongs to the technical field of fish molecular breeding. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a lateolabrax japonicus genome. The reagent is used for detecting genotypes of an SNP site 1, an SNP site 2 and an SNP site 3. The SNP site 1 is located at a 4806373 bp position of a chromosome 4 of a lateolabrax japonicus genome, the SNP site 2 is located at a 16421908 bp position of a chromosome 5 of the lateolabrax japonicus genome, and the SNP site 3 is located at a 7808123 bp position of a chromosome 18 of the lateolabrax japonicus genome. A group of lateolabrax japonicus sex-related SNP molecular markers are screened and used for molecular marker-assisted sex identification and early breeding of lateolabrax japonicus, and a technical tool and a reference basis are provided for rapid screening and cultivation of unisexual lateolabrax japonicus populations with better growth traits.

The present invention relates to a measuring system for the non-invasive, preferably automated, sex determination of embryos in an egg 50 during early embryonic development, particularly before the seventh day of incubation, particularly during incubation, particularly in an incubator with at least one egg tray 40 for holding a plurality of eggs 50 and at least one egg trolley 80 for holding at least one egg tray 40, comprising at least one irradiation unit 10 for irradiating an egg 50 with electromagnetic radiation; at least one sensor unit 20 for detecting electromagnetic radiation transmitted by the egg 50; and an evaluation unit 30 with at least one spectrometer 31 configured to receive the radiation transmitted by the egg 50 and to generate a spectrum of the radiation transmitted by the egg 50.and a data processing unit 33 configured to receive spectra generated by the spectrometer 31 and to store them as a reference spectrum or measurement spectrum; an identification unit 23 for generating identification data by means of which the spectra generated on an egg 50 can be uniquely assigned to the egg 50; and a classification unit 35; wherein the data processing unit 33 is configured to store spectra generated by the spectrometer 31 and associated identification data, and wherein the classification unit 35 is configured to determine the sex of the embryo based on at least one reference spectrum and at least one measurement spectrum. A corresponding method is further specified within the scope of the invention.

The invention provides an ictalurus punctatus sex regulation target, a zbtb38 gene knockdown ictalurus punctatus sex reversal method and application, and belongs to the technical field of sex regulation. According to the invention, the sex control effective regulation target spot zbtb38 gene is successfully screened out, and the shRNA target spot is designed aiming at the coding region of the channel catfish zbtb38 gene, so that compared with CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), the shRNA target spot has the characteristics of easiness in death and low success rate, and the expression inhibition of the channel catfish zbtb38 gene is effectively realized through site-directed gene knock-down. According to the method, the ietalurus punetaus sex reversal model is successfully established, all-female reversed fishes and hermaphrodite reversed fishes are obtained, the method has important value for sex determination mechanism research of the ietalurus punetaus, optimization of an all-male breeding strategy can be reversely guided, a scientific basis is provided for all-male group breeding and breeding cost optimization, and the method has wide application prospects. And finally, the high-quality development of the ictalurus punctatus breeding industry is promoted.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a specific SNP molecular marker for rapidly identifying the genetic sex of Pelochelys cantonensis and application thereof, the SNP molecular marker is selected from at least one of JB_SNP1-3, is located at the 337th, 363rd and / or 392th base of the nucleotide sequence shown in SEQ ID NO. 3 and / or 4, and when being GG, CC and / or TT genotype respectively, the to-be-tested Pelochelys cantonensis individual is male, and when being GT, CA and / or TC genotype respectively, the to-be-tested Pelochelys cantonensis individual is female. The application provides an important molecular tool for the sex identification and sex determination mechanism research of Pelochelys cantonensis, can convert the judgment of the sex of Pelochelys cantonensis into the judgment of the SNP genotype, is favorable for the sex identification of Pelochelys cantonensis in the early growth stage, and has the advantages of rapidness and high efficiency, short sex detection period, large detection flux, small environmental factor interference, high accuracy and reliability of detection results and the like.

This invention discloses an automated system for identifying the sex of fish, belonging to the fields of aquaculture automation and machine vision technology. The automated system for identifying the sex of fish includes: a front-end section comprising a host computer, probes, and an automatic gonad identification system; and a back-end section comprising a sorting conveyor belt. The probes are used to collect fish sex characteristics and transmit the collected images or signals to the host computer. The automatic gonad identification system uses a deep learning model to perform sex identification on the collected images and outputs a sex determination result. The host computer controls the sorting conveyor belt to perform sorting operations based on the determination result. This invention aims to solve the problem of the lack of an integrated automated system in the prior art that can integrate multimodal information acquisition (such as vision and ultrasound), automatic intelligent identification, and real-time linkage with physical sorting actuators.

The invention discloses a DNA molecular marker and an identification method for identifying the sex of a living body of strongylocentrotus intermedius, the nucleotide sequence of the DNA molecular marker is as shown in SEQ ID NO: 1, and the identification method is carried out according to the following steps: cutting the foot of the strongylocentrotus intermedius, and extracting the genome DNA of the strongylocentrotus intermedius; the obtained genome DNA is used as a reaction template, a PCR amplification reaction is carried out, and nucleotide sequences of upstream and downstream primers of the PCR amplification reaction are shown as SEQ ID NO: 2 and SEQ ID NO: 3 respectively; and carrying out agarose gel electrophoresis detection on a PCR reaction product, determining that a specific band with the length of 120bp is amplified as a female individual, and determining that a specific band without an obvious band is a male individual.

The invention relates to the technical field of pelochelys bibroni sex identification, in particular to a molecular marker, a primer group, a kit and a method for identifying pelochelys bibroni sex and application thereof. The molecular marker has a nucleotide sequence as shown in SEQ ID NO: 1, stably exists in a pelochelys bibroni female individual, and is deleted in a male individual. On the basis, the invention designs a specific primer group which can be used for detecting the molecular marker through PCR (Polymerase Chain Reaction) amplification. The invention also provides a kit containing the primer group and a non-diagnostic identification method. According to the method, the sex is judged by detecting whether a PCR amplification product exists or not: the sex is female when a specific band appears, and the sex is male when no band exists. The pelochelys bibroni sex identification method provided by the invention is non-harmful, applicable in the early stage, rapid, efficient and low in cost, provides a reliable molecular tool for artificial breeding and population protection of pelochelys bibroni, and can also provide technical reference for other ZW type sex determining species.

A sexing apparatus 100 includes irradiation means for irradiating each of hen eggs within a predetermined period of time from start of incubation with light having a predetermined wavelength, imaging means for imaging each of the irradiated hen eggs, means for generating a sexing model using, as training data, a result of separately sexing the hen eggs and image data obtained by the imaging of the hen eggs, and means for sexing a new target hen egg by inputting an image data obtained for the target hen egg by the irradiation means and the imaging means into the sexing model.

The application discloses a primer for identifying a genetic sex-specific molecular marker of Tetraspimura quadricornis, which comprises a forward primer and a reverse primer, wherein the nucleotide sequence of the forward primer is shown as SEQ ID NO: 3, and the nucleotide sequence of the reverse primer is shown as SEQ ID NO: 4. The application further discloses a kit comprising the primer, a method for identifying the genetic sex of Tetraspimura quadricornis, and application of the primer, the kit or the method in identifying the genetic sex of Tetraspimura quadricornis. The method of the application is suitable for accurately identifying the genetic sex of Tetraspimura quadricornis in a laboratory or an ornamental fish breeding enterprise. The method for detecting the genetic sex of Tetraspimura quadricornis can be used for sex identification and screening of Tetraspimura quadricornis at different growth stages and in different populations, and is of great significance for studying the sex determination and differentiation mechanism of Tetraspidae and realizing the sex screening.

The present application relates to the field of molecular biology, and more particularly to a genetic sex-specific marker of Pelophylax nigromaculatus and application thereof, the nucleotide sequence of the marker is shown as SEQ ID NO:1, the specific marker provided by the present application can quickly and simply identify the genetic sex of Pelophylax nigromaculatus at each age stage, and is of great significance to the study of the sex determination and differentiation mechanism of Pelophylax nigromaculatus and the realization of the sex control thereof.

Methods and compositions for non-lethal biological sampling of insect larvae are described herein, as are methods and compositions for determining the sex of an insect larvae.

This invention relates to the field of molecular biology, and more specifically to specific markers, primer pairs, detection methods, kits, and applications for insertion mutations in the *Mical2* gene of the striped rock seabream. The specific markers are DNA fragments with non-insertion and insertion-specific markers within the intron region of the *Mical2* gene of the striped rock seabream, the nucleotide sequences of which are shown in SEQ ID NO:1 and SEQ ID NO:2. This invention uses a pair of specific primers to amplify 118 bp and 391 bp bands in individuals with DNA insertion mutations, while only amplifying the 118 bp band in individuals without insertion mutations. The detection method of this invention significantly shortens the identification time, improves detection efficiency, and has important application value in sex determination, high-male-ratio seedling preparation, and family selection of the striped rock seabream.

The invention discloses a female parent RNA interference method based on a parasitic wasp specific developmental window period, and relates to the technical field of insect functional genomics and genetic manipulation, and the method comprises the following steps: systematically comparing microinjection effects of a pre-pupal period and an adult period, determining an efficient and safe window of parasitic wasp female parent RNA interference in the adult period, the method comprises the following steps: selecting a conservative transform gene in an insect sex determination pathway as a target gene to prepare specific double-stranded RNA, and performing targeted microinjection on the belly of a female bee in an adult stage, so that efficient silencing of the target gene is realized on the premise of ensuring high survival rate and normal reproductive capacity of a female parent. According to the method, by defining an efficient and safe operation window of the parasitic wasp female parent RNAi in the adult stage, the technical problems of high death rate, developmental disorder, reproductive power decline and the like caused by injection in the pre-pupal stage are thoroughly solved, the cumulative survival rate of the female parent within 20 days is not lower than 50%, the operation repeatability is high, and a stable and reliable technical normal form is provided for research on the gene function of the parasitic wasp.

The invention relates to the technical field of aquatic genetic breeding, and discloses a specific molecular marker method for identifying the genetic sex of macrobrachium rosenbergii and application, the specific molecular marker method comprises the following steps: S1, obtaining a macrobrachium rosenbergii individual sample, and extracting the genome DNA of the macrobrachium rosenbergii individual; s2, carrying out whole genome re-sequencing on the genome DNA of the female macrobrachium rosenbergii and the genome DNA of the male macrobrachium rosenbergii, and carrying out bioinformatics analysis and screening to obtain candidate specific nucleotide sequences with differences between the male and female macrobrachium rosenbergii and the female macrobrachium rosenbergii; and S3, designing a PCR amplification primer based on the candidate specific nucleotide sequence, and screening to obtain the specific molecular marker for identifying the genetic sex of the macrobrachium rosenbergii. Whole genome re-sequencing is carried out on female macrobrachium rosenbergii and male macrobrachium rosenbergii, specific nucleotide sequences with differences between male and female individuals are obtained based on bioinformatics analysis and screening, specific molecular markers are constructed, and limitation caused by sex judgment only depending on external forms or developmental stages is avoided.

The invention discloses a poplar sex visual detection primer, a probe and crRNA based on RPA-LFD and RPA-CRISPR / Cas 12a and application of the poplar sex visual detection primer, the probe and the crRNA, and belongs to the technical field of gene detection. Specific primers (SEQ ID NO.1 / 2 or SEQ ID NO.5 / 6) are designed on the basis of a poplar MSL sex determination gene, two visual detection modules, namely a lateral flow chromatography test strip module or a CRISPR / Cas 12a signal output module, are combined to form an RPA-LFD detection path combined with an NFO probe and an RPA-CRISPR / Cas 12a detection path based on Cas 12a signal amplification, and sex determination can be rapidly completed under the condition of constant temperature. The two systems can be read by naked eyes through LFD test strips, the detection operation is simple and convenient, the time is short, and the method is suitable for rapid sex identification of the poplar under laboratory and field conditions.

The invention concerns the field of process engineering and relates to a method for in-ovo sex determination in an unopened avian egg, the method for example being used in commercial chicken breeding. The problem addressed by the invention is that of specifying a method by means of which in-ovo sex determination in an avian egg is made possible with a high prediction accuracy. The problem is solved by a method in which the following method steps are carried out: providing a fertilized avian egg incubated for a maximum of 9 days, detecting the position of the blood-carrying cardiovascular system in the avian egg, irradiating the avian egg, acquiring and storing absorption spectra using at least one detector and storage device, detecting and selecting the blood-specific absorption spectra and the non-blood absorption spectra and subsequently compensating the absorption spectra, performing an evaluation by determining at least one value of the oxygenation of the haemoglobin, classifying the blood-specific absorption spectra, determining the sex of the embryo.

This invention belongs to the field of marker-assisted breeding technology, and particularly relates to sex-specific SNP molecular markers and genetic sex identification methods for pearl softshell turtles. The SNP molecular marker is located at the 128th base of the nucleotide sequence shown in SEQ ID NO. 3 and / or 4. When the 128th base is the AA genotype, the tested pearl softshell turtle is male; when it is the AG genotype, the tested pearl softshell turtle is female. The SNP molecular markers provided by this invention offer an important molecular tool for sex identification and sex determination mechanism research. They can transform the determination of the sex trait of a pearl softshell turtle into the determination of the molecular marker genotype, which is beneficial for sex identification in the early stages of pearl softshell turtle growth. This invention has advantages such as speed and efficiency, short sex detection cycle, high throughput, minimal interference from environmental factors, high accuracy and reliability of detection results, and has broad application prospects and social significance in the field of pearl softshell turtle sex identification.

The invention relates to the field of process engineering and concerns a method for in-ovo sex determination on the unopened bird egg, which is used, for example, in commercial chicken farming. The object of the invention is to provide a method by which the in-ovo sex determination of the bird egg is made possible with a high degree of predictive accuracy. The task is solved by a procedure in which the following procedural steps are carried out: - Providing a fertilized bird egg that has been incubated for a maximum of 9 days, - Determining the location of the blood-conducting cardiovascular system in the bird egg, - Irradiating the bird's egg, - Acquisition and storage of absorption spectra with at least one detector and storage device, - Detecting and selecting the blood-specific absorption spectra and the absorption spectra of foreign substances and subsequently compensating the absorption spectra, - Evaluate by determining at least one value of hemoglobin oxygenation, - Classifying blood-specific absorption spectra, - Determining the sex of the embryo.

The invention discloses a method for efficiently editing a sex control gene cyp19a1a in a spermatogonial stem cell (ObSSC) of opsariichthys bidens, which comprises the following steps: aiming at the cyp19a1a gene of opsariichthys bidens, designing four sgRNA (small guide ribonucleic acid) knockout target spots based on a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (clustered regularly interspaced short palindromic repeats) system; the method comprises the following steps: synthesizing sgRNA, and incubating the sgRNA and Cas9 protein at room temperature to form an RNP compound; the RNP compound is further incubated with ObSSC, and is electrically transferred to the ObSSC under the optimal transfection condition. The method disclosed by the invention has the advantages of high efficiency, simplicity, convenience, short period and the like, ensures that exogenous molecules are efficiently introduced into cells while maintaining in-vitro survival and proliferation of the ObSSC, lays a foundation for subsequently obtaining functional gametes carrying edited genes through ObSSC transplantation and analyzing a sex determination mechanism of the functional gametes, solves the fundamental problem of genetic modification cross-generation delivery, and has a wide application prospect. And a new technical path can be provided for fish genetic breeding.

The application discloses a RNAi interference fragment targeting a donkey Zfy gene, a carrier and a preparation method and application thereof, and relates to the technical field of animal molecular genetics, wherein the RNAi interference fragment comprises RNAi-3-F and RNAi-3-R, and the nucleotide sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The RNAi interference fragment provided by the application can target and silence Y chromosome sex determination related genes in male donkey Y spermatogenesis stage key reproductive cells, hinder and interfere with the normal development and physiological functions of Y sperm, regulate the key genes of Y sperm development through specific silencing, realize sex regulation before fertilization, greatly improve the proportion of female individuals of offspring donkeys, and the scheme does not damage target sperm cells and does not reduce sperm activity, and meanwhile, the scheme has the advantages of simple operation and low production cost.

This invention provides a method for breeding large yellow croaker that is entirely female and triploid. First, pseudo-male large yellow croaker germplasm is obtained using sex reversal technology. Then, pseudo-males are identified using molecular markers for sex determination. These pseudo-males are mated with normal females to obtain wholly female fertilized eggs. Hydrostatic pressure treatment is then used to inhibit the expulsion of the second polar body from these fertilized eggs, inducing the formation of wholly female triploid large yellow croaker. Simultaneously, the concentration of the sex reversal reagent, the usage cycle, water temperature, accumulated temperature, pressurization time, pressure value, and hydrostatic pressure treatment duration were all optimized to achieve a 100% triploid rate and a 100% female rate, meeting the requirements for large-scale, industrialized breeding.