190 results about "Sex Determination Process" patented technology

The invention relates to a detection method performed on a maternal serum or plasma sample from a pregnant female, which method comprises detecting the presence of a nucleic acid of foetal origin in the sample. The invention enables non-invasive prenatal diagnosis including for example sex determination, blood typing and other genotyping, and detection of pre-eclampsia in the mother.

The invention relates to a detection method performed on a maternal serum or plasma from a pregnant female, which method comprises the presence of a nucleic acid of fetal origin in the sample. The invention enables non-invasive prenatal diagnosis including, for example, sex determination, blood typing and other genotyping, and detection of pre-eclampsia in the mother.

The invention relates to a tumor cell microsatellite instable state complex amplification system and a detection kit and belongs to the field of biotechnology. In the invention, 8 quasi-monomorphic nucleotide repeated loci with high sensitivity and specificity (including NR-21, NR-22, NR-24, NR-27, BAT-25, BAT-26, MONO-27 and CAT-25) are selected to detect microsatellite instability (MSI). Meanwhile, an amelo (AMEL) is added and a 9-loci multiplex amplification system is constructed, so the result is more accurate. The addition of the gender determining locus can prevent an experiment operator from mixing up samples and delivering wrong results to a certain extent. In the invention, the operation is simple, and large-scale promotion is convenient. With the success of the development of the kit, the method can be used for batch production; the use conditions of the method can be modularized; automatic equipment is applied in the whole process, so the whole process is independent of theoperational experiment of people; and the total detection time is about 6 hours.

The invention relates to a method and apparatus for pre-hatch avian embryo sex determination. In particular, the invention relates to a non-invasive method and apparatus for in-ovo determining the sex of avian species while in the egg and allowing to sort the eggs into groups consisting primarily of either male or female embryos. The method comprises the steps of introducing into the egg an antibody designed to match with a sex specific antigen on the embryo, which antibody is labelled, allowing the labelled antibody to migrate to and bind with the sex specific antigen on the embryo, detecting binding of the labelled antibodies on the embryo using detection means positioned outside of the egg.

The invention relates to a five-color fluorescence labeling multiplex amplification system for analysis of 24 loci of human genome DNA at the same time. 19 autosome loci, 4 Y chromosome loci and 1 sex determination locus are detected at the same time. The system divides the 24 loci into 4 groups, and relates to fluorescence labeling of 5 colors. The fluorescence labeling multiplex amplification system has high sensitivity, under condition that the DNA template amount is 0.12ng, all the 24 loci can be detected, and the system is suitable for direct amplification of blood filter paper and FTA card collecting samples.

The invention provides a single nucleotide polymorphism (SNP) composite detection system and an SNP composite detection method. The SNP composite detection system comprises a group of 48 SNP loci suitable for forensic medicine application, an amplification primer group, a mini-sequencing primer group and a universal chip, and can be used for individual identification, ABO genotyping and sex determination. The invention also provides the SNP composite detection method for the individual identification, the ABO genotyping and the sex determination by using the SNP composite detection system. Byamplifying nucleotide fragments containing the 48 SNP loci of deoxyribonucleic acid (DNA) of a sample to be detected, performing extension reaction on mini-sequencing primers and performing SNP locus-based genotyping on an amplification product by using the universal chip, the capacity of analyzing trace degraded sample DNA is improved, the sample range of a crime scene is expanded, and powerful technical support is provided for the criminal enforcement and administrative enforcement of public security organs.

The invention relates to a poultry breeding technology, in particular to a cultivation method of high-yield native laying chicken. The cultivation method comprises the following steps of: F-series cultivation, backcrossing royal chicken with the synthesis series of parental royal chicken and parental Loman laying chicken, obtaining an underlying group strain containing 75 percent of royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; M-strain breeding cultivation: carrying out cross breeding on the parental royal chicken and the parental Loman laying chicken to obtain an underlying group strain containing 50 percent of the royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; producing commercial generation in matching strains: by taking F strains as parental generation male parent and M strains as parental generation female parent, carrying out sex determination on filial generation by adopting an anal swelling method, wherein the female chicken are commercial-generation laying chicken. The cultivation method can cultivate new species (matching strains) of native laying chicken with high egg yield and excellent egg quality, the egg yield of one commercial chicken after entering into a pheasantry for 72 weeks reaches more than 235 and is improved by 50 percent compared with the egg yield of pure native laying chicken; and the commercial eggs have same appearance quality, protein quality and flavor characteristics with native eggs.

The invention discloses a compound amplification kit for InDel genetic polymorphic sites of human euchromosome and Y chromosome and application thereof. The kit comprises 47 pairs of euchromosome InDel site loca, 2 pairs of Y chromosome InDel site loca and a pair of specific amplification primers of a sex determination gene. The kit can be used for human individual recognition, paternity identification and degraded detection material recognition. The kit comprises 49 InDel sites which are relatively balanced in types and one sex determination gene. By adopting a six-colored STR fluorescence detection system, the kit is higher than the disclosed legal medical InDel detection kit. The kit disclosed by the invention is suitable for detecting Chinese groups. An amplification system of 50 sites is short in amplification fragment, and the amplicon is controlled at 200bp, so that the system is suitable for InDel typing of high degraded detection material; the amplification system improves the detection numbers of sites in the degraded detection material; the two Y-InDel sites introduced into the system plays an auxiliary judging role on the sex determination gene Amelogenin.

The invention discloses a specific molecular marker for acquiring the genders of bighead carps and a screening method and application thereof. The screening method comprises the following steps: firstly, performing reduced-representation sequencing on 5 female bighead carps and 5 male bighead carps by using a 2b-RAD technology to identify a male specific sequence; then, performing genome resequencing and genome assembly on one of the male bighead carps, comparatively searching 2b-RAD specific sequences of the male bighead carps in a male bighead carp genome to obtain a long-fragment male bighead carp specific sequence, and designing a male specific primer. By adopting the screening method, a male specific sequence and a gender specific primer of the bighead carps are screened for the first time, and a PCR (Polymerase Chain Reaction) technology for generic gender determination is built. The method has the advantages of low cost, easiness, rapidness and accuracy in operation, and the like, and a reliable technical measure is provided for the development of a gender marker and gender determination of the bighead carps. The method can be popularized and applied in gender marker development and gender determination research of other fishes.

The invention relates to a PCR amplification primer and a method for rapidly identifying a genetic sex of Chinese softshell turtle. A sequence of the primer is as shown by SEQ ID No.1 and SEQ ID No. 2. The identification method adopts Chinese softshell turtle DNA to be identified as a template and utilizes the primer to perform PCR amplification, the electrophoresis detection is performed for an amplification product, when the amplification product has a strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the female (i.e. the type of sex chromosome is ZZ);and when the amplification product has the strip of 1533bp and the strip of 973bp, identifying the genetic sex of the individual Chinese softshell turtle as the male (i.e. the type of the sex chromosome is ZW). The invention also provides a kit comprising the primer. The PCR amplification primer, the method and the kit are simple, rapid, low in cost, and capable of providing reference evidence for developing a Chinese softshell turtle genetic sex marker, identifying the genetic sex, studying the sex decision mechanism and studying the artificially-controlled Chinese softshell turtle sex differentiation.

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g/mu l and an adjuvant concentration of 50-150 mu g/mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.

The invention discloses a larimichthys crocea genetic sex related SNP (Single Nucleotide Polymorphism) marker and a primer and the application. The larimichthys crocea genetic sex related SNP marker is the sequence shown as in SEQ ID NO:1, and the 201th basic group from the 5' end is T or C. The individual is female larimichthys crocea when the SNP marker is CC genotype, and the individual is malelarimichthys crocea when the SNP marker is CT genotype. By identifying the molecular marker, the SNP marker has the characteristic of rapidly, conveniently, accurately identifying the genetic sex oflarimichthys crocea, moreover, the larimichthys crocea genetic sex related SNP marker also lays foundations for successfully carrying out the work of sex control and breeding of larimichthys crocea, developing monosex cultivation, developing selective breeding of the genome of the larimichthys crocea, and studying the sex determination molecule mechanism.

The invention provides a breeding method of babylonia areolata cross-breeding seeds. Male Thailand babylonia areolata and female Hainan babylonia areolata as well as male Hainan babylonia areolata and female Thailand babylonia areolata are respectively adopted for cross breeding, and through processes of parent babylonia areolata sex determination, parent babylonia areolata of different geographical population cross breeding pairing, parent babylonia areolata nutrition enhacnign culture, oocyst collection and hatching, larva culture, young babylonia areolata culture and the like, the cross-breeding seeds with high growth speed and strong disease resistance are cultured. When the method is used for breeding cross-breeding seeds, the culture diseases are reduced, and the occurrence of diseases such as turgescence diseases and shucking diseases can be avoided. The cross-breeding seed stocking is adopted, the yield is increased by 8.2 to 20.3 percent through being compared with that of the traditional breeding seeds, the production value of per square meter of culture pools is increased by 80 to 189.6 Yuan, and the culture economic benefits are improved; antibiotics are not used in the culture process, the product quality is improved, the cultured product conforms to the pollution-free food standard, positive effects are realized on marine ecological environment protection, and the stable healthy and continuous development of the babylonia areolata culture industry is promoted.

The invention provides a method and system for individual recognition and paternity identification of an unknown sample. The method includes: extracting the DNA of the unknown sample; acquiring the typing result of 17 loci contained by the DNA, i.e. 14 autosome STR loci, 2 Y-chromosome loci, and the sex determination locus Amelogenin, with the STR loci being D6S1043, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, D13S317, D8S1179, D16S539, Penta E, D5S818, vWA, D18S51 and FGA, the Y-STR locus being DYS448, and the Y-chromosome insertion/deletion site being M134; and carrying out individual recognition and paternity identification according to the genotypes of the 17 loci of the unknown sample. The invention also provides the system for individual recognition and paternity identification of the unknown sample. The scheme involved in the invention can realize simultaneous detection of autosome STR loci and Y-chromosome loci, and also can shorten the detection time, thus improving the efficiency of individual recognition.

The invention discloses a local chicken breeding method comprising steps as follows: brooding period: healthy chicks are selected, cocks and hens are divided after sex determination, and the chickens are caged in a 40 cm high mesh bed or bred on a ground with thick padding for brooding until the chickens are 6 weeks old; breeding period: the chickens are put in forest land or mountains for breeding when 7-17 weeks old, the cocks are fed with cock feed for the breeding period, the hens are fed with hen feed for the breeding period, and the hens are reserved through random selection, and the hens are reserved in the ratio of the cocks to the hens being (1:8-1:12; individual caging laying period: the cocks and the hens are caged individually and artificially inseminated until 45 weeks old, and individual and family breeding reservation is carried out according to the 40-week egg production and the early-stage growth speed; forest land or mountain free-range laying period: all the cocks and hens 46-60 weeks old are put in the forest land or mountains, take food freely and mate naturally. The method has the advantages that the breeding effect of local chickens is obviously improved, the survival rate and the breeding rate of the chicks are increased, the individual egg yield and the average egg weight in the laying period are increased, and the percent of pass and the fertility rate of hatching eggs are raised.

The present invention provides methods for non-invasive determination of sex in a fetus or of Y chromosomal frequency abnormalities—indicative of aneuploidy or sex mosaicisms in a fetus—by detecting and determining the relative contribution genetic sequences from the Y chromosome in view of the percent fetal contribution in a maternal mixed sample.

The invention is applicable to the computer vision technology field and provides a method and a system for human face sex characteristic extraction. The method comprises steps of performing pre-processing on an original human face image, performing characteristic extraction on the human face image after pre-processing based on a deep convolutional neural network model and determining sex determination according to the extracted characteristics. The invention can effectively improve the accuracy of sex characteristic extraction and reduces the complexity of the operation during the extraction process.

The invention discloses a real-time quantitative PCR detection primer set and a real-time quantitative PCR detection method of a differentially expressed acipenser dabryanus gonad gene. The real-timequantitative PCR detection primer set comprises an SOX9 primer pair, an FST primer pair, a DF primer pair or a beta-actin primer pair. The real-time quantitative PCR detection method comprises the following steps: extracting RNA from an acipenser dabryanus sample to be detected, using the total RNA as a template, and synthesizing cDNA of the acipenser dabryanus sample to be detected; using the cDNA of the acipenser dabryanus sample to be detected as a template, adding the real-time quantitative PCR detection primer set of the differentially expressed acipenser dabryanus gonad gene, performingreal-time fluorescent quantitative PCR detection, and detecting an amplification product according to an agarose gel electrophoresis result and an amplification product dissolution curve. The primersand the quantitative PCR detection methods of a real-time quantitative PCR target gene and an internal reference gene are suitable for acipenser dabryanus as well as other sturgeons with a similar kinship, and a basis is provided for identifying genders of the sturgeons and researching a gender determining gene and a gender determining mechanism in the future.

The invention discloses a sex determination method for apostichopus japonicas. The method comprises the following steps: (1) taking apostichopus japonicas coelomic fluid, and respectively preparing coelomic fluid supernatant and coelomocyte breaking fluid supernatant; (2) taking the coelomic fluid supernatant to carry out SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), and taking the coelomocyte breaking fluid supernatant to carry out native-PAGE; (3) judging whether SDS-PAGE gel contains a 50kDa protein band or not to determine whether the apostichopus japonicas is male or not, and judging whether a phenoloxidase zymogram after the native-PAGE is carried out contains three bands or not to determine whether the apostichopus japonicas is female or not. The sex determination method has the following advantages: 1) the apostichopus japonicas is slightly harmed; 2) reagent, equipment and technical requirements are simple, and a result is visual; 3) determination speed is high, and handling capacity is high; 4) two technologies are used for respectively determining male and female apostichopus japonicas, and accuracy is 90% or above.