62 results about "Sex Determination Process" patented technology

The invention belongs to the technical field of light polarization analysis, and discloses a method for determining the sex of an embryo in an egg based on polarization image recognition. Comprising the following steps: acquiring a transmitted spectrum image sequence of a hatching egg to be detected at a plurality of polarization angles, wherein the transmitted spectrum image sequence covers a transmitted spectrum image from visible light to a near-infrared band; extracting a polarization sensitive wave band set of the embryonic tissue according to the polarization state change characteristics of the embryo area in the transmitted spectrum image at different polarization angles; constructing an embryo gender characteristic spectrum based on the polarization degree distribution difference and the spatial texture characteristics of each wave band in the polarization sensitive wave band set; determining an optimal wave band combination for sex determination by analyzing the relevance between a polarization anisotropy index of a specific wave band in the embryo sex characteristic spectrum and an embryo development stage; according to the polarization response mode corresponding to the optimal wave band combination, the sex of the embryo in the egg is judged, and the resource investment and the waiting cost are greatly reduced.

The present application discloses a specific DNA fragment for sex identification of Pelteobagrus ussuriensis and a method for sex identification, specifically relating to the technical field of sex identification of Pelteobagrus ussuriensis. The above-mentioned identification method includes the following steps: Dissect 5 female and 5 male Pelteobagrus ussuriensis of a full-sib family to determine the physiological sex; respectively extract DNA from the muscles of female samples and male samples, construct a paired-end genomic DNA library with an insert fragment size of 300 bp, and perform high-throughput sequencing; based on the GWAS and Fst methods, identify that the sex chromosome of Pelteobagrus ussuriensis is chromosome 8, and then screen for Indels within the sex determination region on chromosome 8; screen a 601-bp male-specific DNA sequence and design and synthesize primers; use PCR amplification and agarose gel electrophoresis detection, and the result without specific bands is female, and the one with a 356-bp band is male. This scheme has the advantages of strong specificity, simple primer composition, and more intuitive identification of the sex of Pelteobagrus ussuriensis.

The invention provides a cyprinus carpio sex determining gene ccMD and application thereof in cyprinus carpio sex determination or sex control, the full length of the cyprinus carpio sex determining gene ccMD is as shown in SEQ ID NO.1, the CDS sequence is as shown in SEQ ID NO.2, and the protein coded by the gene is as shown in SEQ ID NO.3. The cyprinus carpio sex determining gene ccMD can be applied to cyprinus carpio sex determination or sex control. Specific primers are designed according to the gene sequence characteristics, and XX, XY and YY carps can be rapidly and accurately identified. The gene is taken as a target gene, a gene editing technology and a gene transfer technology are adopted, hormone treatment is not needed, and a genetically male carp and a physiologically female carp or a genetically female carp and a physiologically male carp can be rapidly obtained.

The invention discloses a method for constructing gene-free edited all-male fish based on a kdm6bb gene knock-down technology, which comprises the following steps: carrying out knock-down treatment on a fish kdm6bb gene by utilizing a CRISPR / Cas9 gene editing technology, and carrying out genotype identification to obtain a kdm6bb heterozygote (kdm6bb + / -); a kdm6bb heterozygous mutant capable of effectively reducing kdm6bb protein expression is obtained through selfing of heterozygous fish; pseudo female fishes with XY sex genetic types are screened through sex molecular markers, the pseudo female fishes are mated with wild XY male fishes, YY sex genetic super-male fishes without kdm6bb mutation are screened, and the YY sex genetic super-male fishes are hybridized with wild XX female fishes, so that all-male fish groups without kdm6bb mutation are obtained, unisexual culture of fishes is realized, sex control breeding efficiency is improved, and breeding cost is reduced. The present invention is widely applicable to aquatic economic fishes on the basis of XX / XY sex determination.

A device for determining the presence of a characteristic of a sample includes a light source for emitting pulsed excitation radiation, a detection device for detecting an autofluorescence radiation emitted by the sample, and a computer-based evaluation device. The detection device is configured to detect the autofluorescence radiation of the sample in a time-resolved manner at different wavelengths by means of time-correlated single photon counting and to provide the evaluation device with two-dimensional data with a wavelength dimension and a time dimenion. The evaluation device is configured to classify the provided data into classes by means of a classifier. The evaluation device is configured to identify specific wavelengths to be prioritized during the classification on the basis of features formed in the time dimension of the data.

Embodiments of the present invention provide systems, methods, and computer readable media with instructions for identifying the gender of a chick. The method includes moving the chick along a path associated with a stimulus for stimulating an open gesture of the chick. The method further includes obtaining one or more images of the chick in the open posture, determining one or more gender-related features of the chick based at least in part on the one or more images, and determining a gender of the chick based at least in part on the one or more gender-related features. One or more portions of the method may include any kind of machine learning techniques, such as supervised machine learning and / or unsupervised machine learning. For example, a supervised machine learning method may be employed to train a neural network to identify a gesture of a chick, identify gender-related features associated with wings of the chick, and / or evaluate gender-related features for gender determination.

The invention relates to a sex-related SNP (Single Nucleotide Polymorphism) molecular marker of lateolabrax japonicus as well as a detection reagent and application thereof, and belongs to the technical field of fish molecular breeding. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a lateolabrax japonicus genome. The reagent is used for detecting genotypes of an SNP site 1, an SNP site 2 and an SNP site 3. The SNP site 1 is located at a 4806373 bp position of a chromosome 4 of a lateolabrax japonicus genome, the SNP site 2 is located at a 16421908 bp position of a chromosome 5 of the lateolabrax japonicus genome, and the SNP site 3 is located at a 7808123 bp position of a chromosome 18 of the lateolabrax japonicus genome. A group of lateolabrax japonicus sex-related SNP molecular markers are screened and used for molecular marker-assisted sex identification and early breeding of lateolabrax japonicus, and a technical tool and a reference basis are provided for rapid screening and cultivation of unisexual lateolabrax japonicus populations with better growth traits.

The invention relates to a rapid mandarin fish sex monitoring system based on ultrasonic image feature recognition, which comprises a multi-modal data acquisition module, an integrated high-frequency ultrasonic probe (10-15MHz), a near infrared spectrum module (850nm) and a visible light camera, and is used for synchronously acquiring an ultrasonic image, blood oxygen distribution data and a mandibular contour image of a mandarin fish belly gonad area. The rapid mandarin fish sex monitoring system based on the multi-mode ultrasonic image and intelligent analysis has a high-precision identification effect, the ultrasonic texture features are combined with near-infrared blood oxygen distribution data, the sex judgment accuracy is improved to be larger than or equal to 95%, and the misjudgment rate is reduced to be smaller than 3%.

The invention provides an efficient enrichment and purification method of adipose-derived stem cell exosomes. According to the invention, the sex determining gene is used for distinguishing and identifying the adipose-derived mesenchymal stem cells from different sources for the first time, and then the culture medium containing specific sex hormone and regulatory factors is used for realizing in-vitro amplification of the adipose-derived mesenchymal stem cells, so that convenience is provided for preparation of the adipose-derived mesenchymal stem cell exosome. According to different sources of adipose-derived stem cell exosomes, different effects of the adipose-derived stem cell exosomes are respectively verified, and the male adipose-derived stem cell exosomes can realize skin injury repair by effectively promoting fibroblast proliferation; the female adipose-derived stem cell exosome can realize nerve injury protection by regulating the nerve conduction velocity and action potential incubation period of an injured part.

To provide a method for determining sex without contacting a beetle larva.SOLUTION: A sex determination method using droppings of a beetle larva includes a determination step which has unevenness of the surface of the droppings as a first index, a proportion of an area of the droppings and length of the droppings as a second index, and determines sex by combining the first index and the second index.SELECTED DRAWING: Figure 4

The invention discloses a large fragment deletion-based specific molecular marker for sex of spinibarbus hollandi and application of the specific molecular marker. According to the marker, whole genome re-sequencing data of male and female individuals of barbus hollandi is compared to a reference genome, and a female specific large fragment deletion region located on a No.43 chromosome (chr43) is screened out. Specific primers are designed according to the deletion region, and rapid and accurate identification of the gender of the spinibarbus hollandi can be realized through PCR amplification and electrophoretogram analysis. The method is suitable for sex identification of spinibarbus hollandi offspring seeds in the early development stage, and provides technical support for research on unisexual breeding, sex control and sex determination mechanism.

The invention belongs to the technical field of molecular marker-assisted breeding, and particularly relates to a gender-specific SNP molecular marker and a genetic gender identification method for florida softshell turtles, the SNP molecular marker is located at the 128 basic group of a nucleotide sequence as shown in SEQ ID NO.3 and / or 4, when the 128 basic group is an AA genotype, a to-be-detected florida softshell turtle individual is male, and when the 128 basic group is an AG genotype, the to-be-detected florida softshell turtle individual is female. The SNP molecular marker provided by the invention provides an important molecular tool for sex identification and sex determination mechanism research, can convert the judgment of the individual sex character of the apalone ferox into the judgment of the genotype of the molecular marker, and is beneficial to sex identification in the early growth stage of the apalone ferox; the method has the advantages of rapidness, high efficiency, short sex detection period, large detection flux, small interference of environmental factors, high accuracy of detection results, good reliability and the like, and has wide application prospects and social significance in the field of sex determination of apalone ferox.

The invention provides a gender determining gene Rpfmd2 of Eriomycorus lividus and application of the gender determining gene Rpfmd2 in biological prevention and control, and belongs to the technical field of biology. According to the invention, after the expression level of the gene is knocked down in the body of the Adelphocoris indica by using an RNA interference technology, the development of the Adelphocoris indica and the specific differentiation process of the female sex are abnormal, and the female Adelphocoris indica even generates a male specific external genital organ; in addition, the egg laying amount of female insects is reduced, and eggs die. The above abnormal phenomena cause death of the lycoris mellifera, and indicate that the Rpfmd2 gene plays an important role in sex differentiation of the lycoris mellifera. In view of high specificity of the gene participating in regulation and control of insect sex determination, the gene can be used as a target spot for accurately preventing and controlling the apis cerinus, and dsRNA targeting the gene can achieve the purpose of effectively preventing and controlling the apis cerinus by disturbing the sex proportion of the apis cerinus and reducing the population number of the apis cerinus. In conclusion, the invention provides a theoretical basis for biological prevention and control of the apis cerana.

The invention relates to the process of extracting a biological sample from a poultry egg (6, 106), in particular for the in-ovo sex determination of a poultry embryo. The invention proposes, in a system (2, 102) in question, a receiving means (4, 104) for receiving at least one poultry egg (6, 106) from which the sample is to be extracted; and a sampling device (8, 108) which is designed to extract a biological sample (12, 112) from an extraction region (10, 110) of the poultry egg (6, 106), said sampling device (8, 108) having a penetration and extraction means (16, 116) which is designed to penetrate the shell (18) of the poultry egg (6, 106) and extract the sample (12, 112) from the extraction region (10, 110) and said receiving means (4, 104) having an aligning device (20, 120) which is designed to position the poultry egg (6, 106) and / or the penetration and extraction means (16, 116) relative to each other such that the penetration and extraction means (16, 116) is aligned adjacently to the extraction region (10, 110).

The invention discloses an LsDHX16 gene of laodelphax striatellus coding RNA helicase and application of the LsDHX16 gene in biological control, and belongs to the technical field of biology. After the expression level of the gene is knocked down in the laodelphax striatellus by using an RNA interference technology, the external genital organ of the female laodelphax striatellus is deformed and the female laodelphax striatellus dies due to ecdysis failure, so that the sex proportion of the laodelphax striatellus is influenced; meanwhile, the development of the amphoteric nymphs is stagnated. The abnormal phenomena show that the LsDHX16 gene plays an important role in sex differentiation and growth processes of laodelphax striatellus. In view of high specificity of the gene participating in regulation and control of insect sex determination, the gene can be used as a target spot for accurately preventing and controlling laodelphax striatellus, and dsRNA targeting the gene can achieve the purpose of effectively preventing and controlling laodelphax striatellus by disturbing the sex proportion of laodelphax striatellus and reducing the population number of laodelphax striatellus. In conclusion, the invention provides a theoretical basis for biological control of laodelphax striatellus.

The present invention relates to a facility for determining the sex of chicks, the facility comprising several identical and parallel processing lines (10), each processing line (10) comprising at least two successive conveyors (16, 17) defining a same direction of movement, the first (16) of these conveyors, in the direction of movement, being referred to as the conveyor (16) for carrying along the chicks and comprising at least one first device (19) for applying a stimulus to a chick in order to prompt the chick to voluntarily deploy its wings, the second (17) of the conveyors, in the direction of movement, being referred to as conveyor (17) for determining the sex of chicks and comprising at least one second device (19) for applying a stimulus to a chick in order to prompt the chick to voluntarily deploy its wings, said facility comprising, at each sex determination conveyor (17): - at least one imaging device (22, 23) for capturing one or more images of the wings of the chick, and - a central unit (U) configured to process the images acquired by said at least one imaging device (22, 23) in order to analyze the configuration of the feathers at the end of at least one of the wings of the chick and determine from this configuration whether it is a male or female chick or if the sex is indeterminate.

The present invention relates to a measuring system for the non-invasive, preferably automated, sex determination of embryos in an egg 50 during early embryonic development, particularly before the seventh day of incubation, particularly during incubation, particularly in an incubator with at least one egg tray 40 for holding a plurality of eggs 50 and at least one egg trolley 80 for holding at least one egg tray 40, comprising at least one irradiation unit 10 for irradiating an egg 50 with electromagnetic radiation; at least one sensor unit 20 for detecting electromagnetic radiation transmitted by the egg 50; and an evaluation unit 30 with at least one spectrometer 31 configured to receive the radiation transmitted by the egg 50 and to generate a spectrum of the radiation transmitted by the egg 50.and a data processing unit 33 configured to receive spectra generated by the spectrometer 31 and to store them as a reference spectrum or measurement spectrum; an identification unit 23 for generating identification data by means of which the spectra generated on an egg 50 can be uniquely assigned to the egg 50; and a classification unit 35; wherein the data processing unit 33 is configured to store spectra generated by the spectrometer 31 and associated identification data, and wherein the classification unit 35 is configured to determine the sex of the embryo based on at least one reference spectrum and at least one measurement spectrum. A corresponding method is further specified within the scope of the invention.

The invention provides an ictalurus punctatus sex regulation target, a zbtb38 gene knockdown ictalurus punctatus sex reversal method and application, and belongs to the technical field of sex regulation. According to the invention, the sex control effective regulation target spot zbtb38 gene is successfully screened out, and the shRNA target spot is designed aiming at the coding region of the channel catfish zbtb38 gene, so that compared with CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), the shRNA target spot has the characteristics of easiness in death and low success rate, and the expression inhibition of the channel catfish zbtb38 gene is effectively realized through site-directed gene knock-down. According to the method, the ietalurus punetaus sex reversal model is successfully established, all-female reversed fishes and hermaphrodite reversed fishes are obtained, the method has important value for sex determination mechanism research of the ietalurus punetaus, optimization of an all-male breeding strategy can be reversely guided, a scientific basis is provided for all-male group breeding and breeding cost optimization, and the method has wide application prospects. And finally, the high-quality development of the ictalurus punctatus breeding industry is promoted.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a specific SNP molecular marker for rapidly identifying the genetic sex of Pelochelys cantonensis and application thereof, the SNP molecular marker is selected from at least one of JB_SNP1-3, is located at the 337th, 363rd and / or 392th base of the nucleotide sequence shown in SEQ ID NO. 3 and / or 4, and when being GG, CC and / or TT genotype respectively, the to-be-tested Pelochelys cantonensis individual is male, and when being GT, CA and / or TC genotype respectively, the to-be-tested Pelochelys cantonensis individual is female. The application provides an important molecular tool for the sex identification and sex determination mechanism research of Pelochelys cantonensis, can convert the judgment of the sex of Pelochelys cantonensis into the judgment of the SNP genotype, is favorable for the sex identification of Pelochelys cantonensis in the early growth stage, and has the advantages of rapidness and high efficiency, short sex detection period, large detection flux, small environmental factor interference, high accuracy and reliability of detection results and the like.

This invention discloses an automated system for identifying the sex of fish, belonging to the fields of aquaculture automation and machine vision technology. The automated system for identifying the sex of fish includes: a front-end section comprising a host computer, probes, and an automatic gonad identification system; and a back-end section comprising a sorting conveyor belt. The probes are used to collect fish sex characteristics and transmit the collected images or signals to the host computer. The automatic gonad identification system uses a deep learning model to perform sex identification on the collected images and outputs a sex determination result. The host computer controls the sorting conveyor belt to perform sorting operations based on the determination result. This invention aims to solve the problem of the lack of an integrated automated system in the prior art that can integrate multimodal information acquisition (such as vision and ultrasound), automatic intelligent identification, and real-time linkage with physical sorting actuators.

The present invention provides rapid genomic diagnostic methods of in vitro produced embryos. Cell-free DNA can be obtained from the culture medium of blastocysts, and then added to a LAMP or PCR amplification reaction to detect the presence or absence of a certain DNA sequence. Colorimetric or fluorescent indicators such as metallochromatic dyes, intercalating dyes, or pH indicator dyes can be added to the reaction so that amplification can be tracked without additional steps. Provided are methods of sex determination and detection of a specific gene edit, as well adverse or desirable phenotypes. Methods of genotyping using cell-free DNA are also provided.

The invention relates to the technical fields of bioanalytics and agriculture and concerns a method for opening eggs. The object of the present invention is to provide a method for opening an egg in which the deposition of chemical or biochemical reaction and / or erosion products of the calcareous shell and the outer shell membrane on the inner shell membrane is avoided.This problem is solved by a method for opening an egg, in which a substantially closed channel structure is created in the calcareous shell of an egg in a surface area located essentially above the air chamber by laser ablation of the calcareous shell material. Subsequently, at least two perforations are created in the calcareous shell and the outer shell membrane, at least in the local area of ​​the created channel structure. Finally, the calcareous shell and the outer shell membrane are removed in a single operation. The method can be used, for example, in the spectroscopic in-ovo sex determination of poultry or in commercial egg production.

The present invention discloses male molecular marker primers of Odontobutis sinensis and their applications. The male molecular marker primers of Odontobutis sinensis are primer STLM2 or primer STLM9. The primer STLM2 includes upstream primer STLM2_F2 and downstream primer STLM2_R2. The primer STLM9 includes upstream primer STLM9_F2 and downstream primer STLM9_R2. The male molecular markers STLM2.2 or STLM9.2 of Odontobutis sinensis and a method for sex identification of Odontobutis sinensis are also disclosed, as well as the applications of the primers or the method in sex identification of Odontobutis sinensis. These primers have good specificity and can quickly and accurately identify the sex of Odontobutis sinensis, breaking through the technical bottleneck that the sex of early fry of Odontobutis sinensis cannot be identified. The primers and method of the present invention have important scientific research value and practical application value for sex determination and sex control of Odontobutis sinensis.

The invention discloses a DNA molecular marker and an identification method for identifying the sex of a living body of strongylocentrotus intermedius, the nucleotide sequence of the DNA molecular marker is as shown in SEQ ID NO: 1, and the identification method is carried out according to the following steps: cutting the foot of the strongylocentrotus intermedius, and extracting the genome DNA of the strongylocentrotus intermedius; the obtained genome DNA is used as a reaction template, a PCR amplification reaction is carried out, and nucleotide sequences of upstream and downstream primers of the PCR amplification reaction are shown as SEQ ID NO: 2 and SEQ ID NO: 3 respectively; and carrying out agarose gel electrophoresis detection on a PCR reaction product, determining that a specific band with the length of 120bp is amplified as a female individual, and determining that a specific band without an obvious band is a male individual.

The invention relates to the technical field of pelochelys bibroni sex identification, in particular to a molecular marker, a primer group, a kit and a method for identifying pelochelys bibroni sex and application thereof. The molecular marker has a nucleotide sequence as shown in SEQ ID NO: 1, stably exists in a pelochelys bibroni female individual, and is deleted in a male individual. On the basis, the invention designs a specific primer group which can be used for detecting the molecular marker through PCR (Polymerase Chain Reaction) amplification. The invention also provides a kit containing the primer group and a non-diagnostic identification method. According to the method, the sex is judged by detecting whether a PCR amplification product exists or not: the sex is female when a specific band appears, and the sex is male when no band exists. The pelochelys bibroni sex identification method provided by the invention is non-harmful, applicable in the early stage, rapid, efficient and low in cost, provides a reliable molecular tool for artificial breeding and population protection of pelochelys bibroni, and can also provide technical reference for other ZW type sex determining species.

The invention relates to the technical field of criminal science, and particularly discloses application of a fatty acid marker in sex determination of people with fingerprints left. According to the method, fatty acids are used as markers, the relative content of 13 fatty acids in fingerprints is measured, the fingerprint-left-person gender determination model is constructed in combination with trained machine learning to identify the gender of the fingerprint-left-person, and the identification method has the advantages of being easy and convenient to operate, high in identification accuracy, wide in applicability and the like. And the overall fingerprint gender classification accuracy can reach 90%. The sex identification method for the person with the fingerprints left has the advantages of low dependence on fingerprint integrity, small interference by age factors, low requirement on instrument sensitivity, capability of carrying out biological information mining on overlapped, fuzzy and incomplete fingerprints which are difficult to identify, and wide applicability.

According to the InDel specific molecular marker for identifying the genetic sex of the megalobrama amblycephala and the application, a primer pair is designed, sample genome DNA serves as a template, the genetic sex of the megalobrama amblycephala can be identified only through PCR and agarose gel electrophoresis, consumed time is short, damage to fish bodies is small, limitation of fish ages is avoided, the InDel specific molecular marker can be applied to the whole life cycle of the megalobrama amblycephala, and the application range of the InDel specific molecular marker is wide. The method is convenient and rapid, has higher accuracy, and is especially suitable for rapid sex determination of a large number of samples. The method provides basic data for further mining of the sex determining gene of the megalobrama amblycephala, and lays a foundation for sex control breeding of the megalobrama amblycephala.

A sexing apparatus 100 includes irradiation means for irradiating each of hen eggs within a predetermined period of time from start of incubation with light having a predetermined wavelength, imaging means for imaging each of the irradiated hen eggs, means for generating a sexing model using, as training data, a result of separately sexing the hen eggs and image data obtained by the imaging of the hen eggs, and means for sexing a new target hen egg by inputting an image data obtained for the target hen egg by the irradiation means and the imaging means into the sexing model.

The invention provides a method for obtaining polytype male and female interstitial Pingewei, and belongs to the technical field of arthropod reproductive mode and phenotype regulation, and the method comprises the following steps: feeding Pingewei carrying Ricktsia for several days by using a rifampicin solution, and feeding eggs to the Pingewei carrying Ricktsia for 24 + / -1h after feeding the rifampicin solution for parasitism, so as to obtain the polytype male and female interstitial Pingewei, and obtaining the polytype male and female interstitial Pingewei, so as to obtain the polytype male and female interstitial Pingewei, and the polytype male and female interstitial Pingewei. On the second day, the third day and the fourth day after the rifampicin solution is fed, polytypic male and female body surface types appear on the offspring, and the male and female body small bees are obtained. According to the method disclosed by the invention, the polytype male and female interbody Pingketsia is obtained by partially removing a symbiotic bacterium Rickketsia in the Pingketsia, so that a model for interaction between the Rickketsia and the host Pingketsia is favorably established, a foundation is laid for researching sex determination and differentiation of the host, and the research on the origin evolution of the sex type II of the insect and the formation reason of the male and female interbody is facilitated.