Primer for detecting CAMV35S genes, relevant kit and detecting method

Abstract

The invention discloses a primer for detecting CAMV35S genes, a relevant kit and a detecting method. The primer for detecting the CAMV35S genes comprises an outer primer F3, an outer primer B3, an inner primer FIP and an inner primer BIP, and the nucleotide sequences of the primer are respectively shown as SEQ ID NO.1-4. The primer can judge whether target matter exists or not according to amplification, and has high specificity. The kit in the invention can rapidly diagnose the CAMV35S genes, can amplify at only a constant temperature without special reagents or equipment, has low detection cost and is suitable for popularization and application.

Description

technical field

[0001] The invention relates to the field of inspection and detection, in particular to a CAMV35S gene detection primer, a corresponding kit and a detection method. Background technique

[0002] CAMV35S gene is the 35S promoter of cauliflower mosaic virus, which is widely used in transgenic crops such as transgenic soybean, corn, rape, potato and tomato.

[0003] In order to strengthen the supervision and management of genetically modified products, a variety of genetically modified component detection methods have been developed, from protein-based ELISA detection technology to nucleic acid-based polymerase chain reaction (PCR) technology and the rapidly developing gene chip technology. Among them, PCR detection technology is the most general because of its sensitivity, specificity, and high efficiency. This technology is identified by detecting exogenous nucleic acid fragments contained in genetically modified products. The emergence of GM technology not o...

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