Rapid diagnostic kit for staphylococcus aureus gene based on loop-mediated isothermal amplification technology and detecting method thereof
A rapid diagnosis technology for Staphylococcus aureus, which is applied in the direction of microorganism-based methods, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of the genetic rapid diagnostic kit for detecting Staphylococcus aureus, etc., and achieve Significant color difference, high specificity, rapid amplification effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] The preparation of embodiment 1 kit
[0042] (1) Synthesize oligodeoxynucleic acid primers by DNA synthesizer according to the following sequence:
[0043] Outer primer F3: TTTTCATAATCRATCACTGGAC, as shown in SEQ ID NO: 1;
[0044] Outer primer B3: TTTAACAGCTAAAGAGTTTGGT, as shown in SEQ ID NO: 2;
[0045] Internal primer FIP: ACAATAATAACGAGGTYATTGCAGCTTTTCTTGAACACTTTCATAACAGGTAC, as shown in SEQ ID NO: 3;
[0046] Internal primer BIP: CCTTCAGCAAGCTTTAACTCATAGTTTTTCAGATAGCATGCCATACAGTC, as shown in SEQ ID NO: 4;
[0047] (2) Purchase DNA polymerase: Bst DNA polymerase (Large Fragment). Put in container.
[0048] (3) Preparation of reaction solution: the reaction solution contains 2mmol / LdNTP, 25mmol / L Tris-Cl, 12.5mmol / L potassium chloride, 12.5mmol / L ammonium sulfate, 10mmol / L magnesium sulfate, 0.125% TritonX-100, 1mol / L L betaine, 2 mol / L each of the inner primers FIP / BIP and 0.25 mol / L each of the outer primers F3 / B3 were placed in the container.
[0049] (4) ...
Embodiment 2
[0060] The preparation of embodiment 2 kit
[0061] The formula of the reaction solution is: the reaction solution contains 1.8mmol / L dNTP, 20mmol / L Tris-HCl, 10mmol / L potassium chloride, 10mmol / L ammonium sulfate, 8mmol / L magnesium sulfate, 0.1%TritonX-100, 0.8mol / L betaine, each 1.6mol / L of inner primer FIP / BIP and each 0.2mol / L of outer primer F3 / B3;
[0062] The formula of the sample pretreatment solution is as follows: the sample pretreatment solution contains 10 mmol / L Tris-HCl with pH 8.0, 1 mmol / L EDTA and 1% Triton X-100.
[0063] Others are the same as embodiment 1.
Embodiment 3
[0064] Application of embodiment 3 Staphylococcus aureus gene rapid diagnosis kit
[0065] 1 Materials and methods
[0066] 1.1 Materials
[0067] 1.1.1 Strains
[0068] There are 22 strains used in our company, mainly from the American Standard Biological Collection Center,
[0069] China Institute for the Control of Pharmaceutical and Biological Products and clinical separation. See Table 1 for details.
[0070] Table 1 strain name and source
[0071] strain source
Strain and serial number
American Standard Biological Collection
Center (ATCC)
Staphylococcus aureus (6538), Salmonella (14028, 13076,
13311), Listeria (7466, 25401);
Preservation of Medical Microorganisms
Management Center (CMCC)
Salmonella (50115);
Clinical isolates
Staphylococcus aureus, Vibrio parahaemolyticus, Shigella, enterocolitis
Yersinia, Salmonella choleraesuis, Salmonella enteritidis, Salmonella typhimurium
Bacteri...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com