Primer group and kit for detecting Roundup Ready transgenic soybeans

Abstract

The invention discloses a primer group and a kit for detecting Roundup Ready transgenic soybeans. The kit consists of the primer group, Bst deoxyribonucleic acid (DNA) polymerase, a stabilizing solution, a reaction solution, a developing solution and a positive control solution. In the kit, six segments and four primers are utilized; and the kit has high specificity according to the condition that whether the existence of target materials can be judged or not through amplification. The quick diagnostic kit provided by the invention has high speed, high efficiency and high sensitivity, can be subjected to an amplification reaction only by a constant temperature without the usage of special reagents and equipment, has low detection cost and is easy and convenient to identify; pyrophosphate ions precipitated from deoxyribonucleoside triphosphate (dNTP) is combined with Mg<2+> in the reaction solution; the produced byproduct, namely magnesium pyrophosphate is precipitated and can be observed and identified through naked eyes; and after the developing solution is added, the kit has obvious development differences of negative and positive results and is more obvious and reliable. A soybean endogenous gene lectin is used as an internal label, so that errors and false negatives caused by the extraction of nucleic acid are prevented; and real-time monitoring is performed, so that detection time is greatly reduced, and human power and material resources are saved.

Description

technical field

[0001] The invention relates to a biological detection reagent, in particular to a roundup ready transgenic soybean detection primer set and a diagnostic kit thereof. Background technique

[0002] The glyphosate-resistant soybean variety developed by Monsanto in 1994 transformed the Agrobacterium tumefaciens The gene encoding 5-enolpyruvate shikimate-3-phosphate synthase (CP4 EPSPS) can resist the inhibition of the herbicide glyphosate on the shikimate synthesis pathway, allowing transgenic soybeans to grow normally after spraying the herbicide. The recipient of this variety is soybean variety A5403, which integrates exogenous CP4 EPSPS gene, E35S promoter and NOS terminator into the transgenic soybean genome. This variety is currently the transgenic soybean variety with the largest planting area, and is widely used in the processing and production of edible oil and food.

[0003] In order to strengthen the supervision and management of genetically modified...

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