Visualized rapid detection kit for carp dropsy virus and detection method thereof

Abstract

The invention discloses a visualized rapid detection kit for carp dropsy virus and a detection method thereof. The visualized rapid detection kit for the carp dropsy virus includes a virus DNA extraction reagent and a reaction reagent, the reaction reagent contains a nucleic acid primer set used for detecting amplification of the carp dropsy virus, and the primer set contains a primer CEV-F3, a primer CEV-B3, a primer CEV-BFIP, a primer CEV-BIP, a primer CEV-LpF and a primer CEV-LpB. The rapid detection kit establishes a set of optimized isothermal amplification reaction system, the qualitative detection of the carp dropsy virus is thus simpler, faster, high in specificity and high in sensitivity, and the kit is the first kit used for detecting the carp dropsy virus, the problem that there is no detection method for the carp dropsy virus is solved, and has high scientific and economic value.

Description

technical field

[0001] The invention belongs to the field of rapid detection of target gene fragments, and in particular relates to a visual rapid detection kit of carp edema virus and a detection method thereof. Background technique

[0002] Carp edema virus (Carp edema virus, CEV) is the pathogen that causes carp edema disease and koisleepy disease (Koisleepy disease, KSD), seriously endangers the healthy breeding of carp and koi, and the mortality rate that disease causes is as high as 80-100%. Carp edema disease is caused by carp edema virus belonging to the Poxviridae family. The disease has spread rapidly around the world and was first discovered in China in 2016. During the occurrence of the disease, the sick fish were unconscious under the water surface or lying on the bottom of the pool. The main symptoms were enophthalmos, gill hyperplasia and anal ulcerative inflammation, and they died within 2 weeks after the onset.

[0003] Carp edema virus is the pathogen of e...

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