A kind of microfluidic chip and preparation method thereof

A microfluidic chip and flow channel technology, applied in chemical instruments and methods, laboratory containers, instruments, etc., can solve the problems of low sensitivity of glycosylated hemoglobin, complex detection process, long detection time, etc., and achieve low background influence. , Accurate results and easy operation

Active Publication Date: 2019-06-11
深圳市海拓华擎生物科技有限公司
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The prior art diagnostic method for glycosylated hemoglobin has the problems of low sensitivity, poor repeatability, obvious interference, and the existing glycosylated hemoglobin detection supporting equipment is expensive, the detection process is complicated, and the detection time is long.

Method used

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  • A kind of microfluidic chip and preparation method thereof

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Embodiment 1

[0030] Preparation of microfluidic chip and its application in the determination of HbA1c.

[0031] 1. Chip structure:

[0032] refer to figure 1 , a microfluidic chip, including a substrate 1, a sampling part 2 and a bottom cover 3; the bottom cover 3 is set under the substrate 1; the substrate 1 is provided with a sampling chamber 11, a pretreatment area 12, a detection chamber 13 and The reaction zone 14; the sampling part 2 is embedded in the sampling chamber 11 in a detachable manner; the sampling part 2 includes a sample inlet 21, a sample holding chamber 22 and a sample outlet 23; the sample inlet 21, the sample outlet 23 Communicate with the sample holding chamber 22 respectively; the pretreatment area 12 includes a first pretreatment reagent chamber 121, a second pretreatment reagent chamber 122, a pretreatment chamber 123 and an air chamber 124; the first pretreatment reagent chamber 121 passes through the first pretreatment reagent chamber The zone flow channel 12...

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Abstract

The invention discloses a micro-fluidic chip which comprises a substrate, a sampling part and a bottom cover, wherein a sample feeding cavity, a pre-treating region, a detection cavity and a reaction region are formed in the substrate; the sampling part comprises a sample feed inlet, a sample accommodating cavity and a sample outlet; the pre-treating region comprises a pre-treating reagent cavity, a pre-treating cavity and a gas cavity; the reaction region comprises a reaction reagent cavity and a reaction cavity; elastic cavity walls are formed on the pre-treating reagent cavity, the gas cavity, the reaction reagent cavity and the reaction cavity; a transparent panel is arranged on the upper surface of the detection cavity and a transparent bottom plate is arranged on the lower surface of the detection cavity; and the transparent panel and the transparent bottom plate correspond to each other. Based on a transmitting turbidimetry, according to the micro-fluidic chip, the content of a to-be-detected component in a sample is quickly and quantitatively detected by means of a micro-fluidic chip technology, so that the micro-fluidic chip has the characteristics of being easy to operate, high in sensitivity and accurate in result. A preparation method of the micro-fluidic chip comprises the steps of preparing a reagent; and assembling the chip.

Description

technical field [0001] The invention relates to a microfluidic chip and a preparation method thereof, belonging to the technical field of chip detection. Background technique [0002] In a normal human body, there are three hemoglobins: HbA, HbF, and HbA2, while adult red blood cells mainly contain HbA. When hemoglobin is separated by chromatography, three sugar-containing components can be eluted, namely: HbA1a, HbA1b, and HbA1c, which are collectively called glycated hemoglobin. HbA1c is glycosylated hemoglobin, and the other two are glycosylated hemoglobin formed from other sugars. Among them, the amount of HbAlc is the largest, and it is generally expressed as HbAlc when reflecting the blood sugar level. HbAlc is formed by the combination of HbA and glucose during the metabolic process, so it can accurately reflect the glucose level in the blood. [0003] The combination of blood sugar and hemoglobin to form glycosylated hemoglobin is an irreversible reaction, which is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N33/577
CPCB01L3/5027B01L2200/10B01L2300/0861G01N33/577
Inventor 何毅
Owner 深圳市海拓华擎生物科技有限公司
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