A kind of microfluidic chip and preparation method thereof
A microfluidic chip and flow channel technology, applied in chemical instruments and methods, laboratory containers, instruments, etc., can solve the problems of low sensitivity of glycosylated hemoglobin, complex detection process, long detection time, etc., and achieve low background influence. , Accurate results and easy operation
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[0030] Preparation of microfluidic chip and its application in the determination of HbA1c.
[0031] 1. Chip structure:
[0032] refer to figure 1 , a microfluidic chip, including a substrate 1, a sampling part 2 and a bottom cover 3; the bottom cover 3 is set under the substrate 1; the substrate 1 is provided with a sampling chamber 11, a pretreatment area 12, a detection chamber 13 and The reaction zone 14; the sampling part 2 is embedded in the sampling chamber 11 in a detachable manner; the sampling part 2 includes a sample inlet 21, a sample holding chamber 22 and a sample outlet 23; the sample inlet 21, the sample outlet 23 Communicate with the sample holding chamber 22 respectively; the pretreatment area 12 includes a first pretreatment reagent chamber 121, a second pretreatment reagent chamber 122, a pretreatment chamber 123 and an air chamber 124; the first pretreatment reagent chamber 121 passes through the first pretreatment reagent chamber The zone flow channel 12...
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