Stable modification method of cellulose membrane used for protein chip synthesis

A technology of cellulose membrane and modification method, which is applied in the direction of adding inorganic compounds and non-polymer organic compounds, etc., can solve the problems of high reaction background and easy breakage, and achieve the effect of reducing the effect of background.

Active Publication Date: 2015-02-11
BEIJING PEPMAGIC BIOTECH CORPORATION LTD
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Problems solved by technology

[0004] In order to solve the above technical problems, the present invention provides a more stable cellulose membrane surface modification method, the cellulose membrane modified by the method solves the problems of easy breakage and high reaction background of traditional modification methods

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  • Stable modification method of cellulose membrane used for protein chip synthesis
  • Stable modification method of cellulose membrane used for protein chip synthesis

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Embodiment Construction

[0045] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0046] The present invention is a kind of stable modification method for the cellulose film of protein chip synthesis, and this method comprises the following steps:

[0047] 1) with 20ml 25% trifluoroacetic acid (TFA) / dichloromethane (CH 2 Cl 2 ) solution to rinse the blank quantitative filter paper twice, each time for 20 minutes; wherein, the model of the blank quantitative filter paper is Whatman50, and the volume ratio of trifluoroacetic acid / dichloromethane solution is 1:4.

[0048] 2) After carrying out the above steps 1), the filter paper was washed with 20ml of dichloromethane (CH 2 Cl 2 ) rinse the filter paper twice, 2 minutes each time.

[0049] 3) Soak an...

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Abstract

The invention relates to a stable modification method of a cellulose membrane used for protein chip synthesis. The cellulose membrane surface modification method is characterized in that the microscopic hydroxy (OH) structure on the surface of the cellulose membrane is changed through a chemical process, and is stably connected to 11-AUN in an ether bond (-O-) form to make the obtained cellulose membrane be used as a polypeptide array synthesis basement membrane. The obtained cellulose membrane can be used for synthesizing protein chips and antibody chips. The cellulose membrane modified through the method solves problems comprising easy fracture, high reaction background and the like of traditional modification methods. Polypeptides on the modified membrane are still maintained stable and the background influence of the surface of the membrane to a polypeptide array can be well reduced even under a highly acidic condition.

Description

technical field [0001] The invention relates to a fiber membrane surface modification technology, in particular to a stable modification method for a cellulose membrane used for protein chip synthesis. Background technique [0002] In the post-genome era, scientists have shifted their research focus from genomics to functional proteomics. Proteomics mainly studies the composition and organization of cellular proteins, protein-protein interactions and their relationship with disease pathogenesis. High-throughput peptide array technology is one of the key and best technologies for studying the interaction between proteins and other biomacromolecules such as other proteins, nucleic acids and polysaccharides. Polypeptide arrays use automated instruments to synthesize hundreds of peptides on solid-phase supports (glass slides, cellulose membranes, optical discs, etc.) simultaneously, and the polypeptide spots exist in the form of arrays on the solid-phase supports At present, p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D21H17/66D21H17/07
Inventor 赵树民鲍勇刚石松传
Owner BEIJING PEPMAGIC BIOTECH CORPORATION LTD
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