Primer group for detecting Yersinia pestis, rapid diagnosis kit and detection method

A rapid diagnosis and kit technology, applied in the field of biological identification of Yersinia pestis, to achieve the effects of mild reaction conditions, improved specificity and high detection sensitivity
CN101824468BActive Publication Date: 2012-10-10GUANGZHOU HUAFENG BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU HUAFENG BIOTECH
Publication Date
2012-10-10

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Abstract

The invention discloses a primer group for detecting Yersinia pestis, a rapid diagnosis kit and a detection method, wherein the primer group consists of the following four primers: an external primer F3, an external primer B3, an inner primer FIP and an inner primer BIP. The kit consists of the primer group, Bst DNA polymerase, sample pretreatment solution, stabilizing solution, reaction solution, colored solution and positive control solution, and the seven kinds of solutions are placed in a vessel. Both the primer group and the kit can detect the Yersinia pestis with high efficiency and high specificity, as well as are based on loop-mediated isothermal amplification technology, apply six segments, four primers and one constant temperature, complete an amplification reaction within 1 hour, and are low in detection cost, short in detection time, high in yield and specificity, obvious in negative and positive result color developing difference, high in verification rate, obvious and reliable in verification.
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Description

Technical field

[0001] The invention relates to the biological identification of Yersinia pestis, in particular to a primer set for detection of Yersinia pestis, a rapid diagnostic kit and a detection method. Background technique

[0002] Yersinia (Yersinia) is now classified as Enterobacteriaceae. It is the pathogen of animal infectious diseases. Humans get sick through contact with infected animals or contaminated food. At present, the genus Yersinia is roughly divided into Yersinia pestis, Y. pseudotuberculosis (Y. pseudotuberculosis), Y. enterocolitica (Y. entererocolitica), and Yersinia intermedia (Yersinia pestis). Y.intermedia), the first three species are highly pathogenic to humans. Among them, Yersinia pestis causes a natural foci disease in rodents, which is highly infectious and has a high mortality rate, which is easy to cause a pandemic.

[0003] At present, there are a variety of detection methods for Yersinia pestis, from the national standard (GB / T 18936-2003), w...

Claims

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