Primer group for testing Roundup Ready transgenic soy bean EPSPS gene, rapid diagnosis kit and testing method thereof

A technology for rapid diagnosis of transgenic soybeans, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of unseen detection of EPSPS gene rapid diagnostic kits for transgenic crops, and achieve significant color difference , high yield and low detection cost
CN101838685BActive Publication Date: 2012-10-03GUANGZHOU HUAFENG BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU HUAFENG BIOTECH
Publication Date
2012-10-03

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Abstract

The invention discloses a primer group for testing Roundup Ready transgenic soy bean EPSPS gene based on the loop-mediated isothermal amplification of DNA (LAMP), a rapid diagnosis kit and a testing method thereof. The kit comprises the primer group, Bst DNA polymerase, stabilizing solution, reacting solution, developing solution and positive control solution. The kit applies six sections and four primers, can judge whether the target substance is existent or not according to the amplification, so that the kit has a high specificity. The rapid diagnosis kit of the invention is rapid, efficient and sensitive; the amplification reaction can be realized only needing constant temperature without the special agent or equipment; the testing cost is low and the test process is simple. Pyrophosphate ions separated from the dNTP are combined with Mg<2> in the reaction solution to generate the byproduct, i.e. magnesium pyrophosphate deposition, which can be observed and appraised by viewing; and the developing differences of the negative and positive results are obviously by adding the developing solution, and the results are more obvious and reliable.
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Description

technical field

[0001] The invention relates to a biological detection reagent, in particular to a roundup ready transgenic soybean EPSPS gene detection primer set, a rapid diagnostic kit and a detection method. Background technique

[0002] The glyphosate-resistant soybean variety developed by Monsanto in 1994 has transformed the gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (CP4EPSPS) from the microorganism Agrobacterium tumefaciens, which can resist the herbicide glyphosate. Inhibition of the shikimic acid synthesis pathway allowed transgenic soybeans to grow normally after spraying herbicides. The recipient of this variety is soybean variety A5403, and the exogenous CP4EPSPS gene, E35S promoter and NOS terminator are integrated in the transgenic soybean genome. This variety is currently the transgenic soybean variety with the largest planting area, and is widely used in the processing and production of edible oil and food.

[0003] In order to strengthen th...

Claims

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