Primer pairs, method and fast diagnostic kit for detecting red-spotted grouper nervous necrosis viruses
A technique for red-spotted grouper and nerve necrosis, which is applied in the field of biological identification of aquatic biological diseases, and achieves the effects of mild reaction conditions, reduced background influence and improved specificity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0064] Example 1 Primer set for detection of Epinephelus Nervous Necrosis Virus and effectiveness detection
[0065] In this example, based on the published genome sequence of the Epinephelus Nervous Necrosis Virus, the specific sequence of the Epinephelus Nervous Necrosis Virus gene is selected, and then analyzed and designed to be used in the LAMP detection technology, which can specifically identify red The specific primer set of Epinephelus nervous necrosis virus, the primer set consists of the following four primers:
[0066] The nucleotide sequence of the inner primer FIP is shown in SEQ ID NO:1;
[0067] The nucleotide sequence of the inner primer BIP is shown in SEQ ID NO: 2;
[0068] The nucleotide sequence of the outer primer F3 is shown in SEQ ID NO: 3;
[0069] The nucleotide sequence of outer primer B3 is shown in SEQ ID NO:4.
[0070] In this example, 8 positive fishes and 2 healthy fishes (such as figure 1 As shown), and then separately extract the RNA of the brain...
Example Embodiment
[0072] Example 2 Preparation of a rapid diagnostic kit
[0073] The rapid diagnosis kit of this embodiment can be used for ring-mediated isothermal amplification technology to quickly diagnose whether the sample contains Epinephelus Nervous Necrosis Virus. The kit consists of a primer set for detecting Epinephelus Nervous Necrosis Virus, LAMP The reaction solution, Bst DNA polymerase, positive control substance, color developing solution and foam board are composed of:
[0074] (1) Primer set for the detection of Epinephelus Nervous Necrosis Virus: DNA synthesizer, 1 tube, inner primer F3, outer primer B3, inner primer FIP and inner primer BIP, inner primer FIP, its nucleotide sequence As shown in SEQ ID NO: 1; inner primer BIP, its nucleotide sequence is shown in SEQ ID NO: 2; outer primer F3, its nucleotide sequence is shown in SEQ ID NO: 3; outer primer B3, its The nucleotide sequence is shown in SEQ ID NO: 4, and the concentration of the above 4 primers is 10uM;
[0075] (2) C...
Example Embodiment
[0089] Example 3 Preparation of a rapid diagnostic kit
[0090]The rapid diagnosis kit of this embodiment can be used for ring-mediated isothermal amplification technology to quickly diagnose whether the sample contains Epinephelus Nervous Necrosis Virus. The kit consists of a primer set for detecting Epinephelus Nervous Necrosis Virus, LAMP The reaction solution, Bst DNA polymerase, positive control substance, color developing solution, Trizol, chloroform, isopropanol, 70% ethanol, RNase-free water, RT reaction solution, reverse transcriptase and foam plate composition, among them:
[0091] (1) Primer set for the detection of Epinephelus Nervous Necrosis Virus: DNA synthesizer, 1 tube, inner primer F3, outer primer B3, inner primer FIP and inner primer BIP, inner primer FIP, its nucleotide sequence As shown in SEQ ID NO: 1; inner primer BIP, its nucleotide sequence is shown in SEQ ID NO: 2; outer primer F3, its nucleotide sequence is shown in SEQ ID NO: 3; outer primer B3, its Th...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap