Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rockfish viral nerve necrosis virogene diagnostic kit and detecting method thereof

A nerve necrosis and gene diagnosis technology, which is applied in the field of grouper virus nerve necrosis virus gene diagnosis kit and detection, can solve problems such as difficulty in popularization, troublesome material preparation, and low sensitivity, so as to improve scientific management efficiency and avoid virus Spread the popularity and ensure the effect of rapidity

Inactive Publication Date: 2004-12-15
SUN YAT SEN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some of these detection methods have high technical requirements, long detection time, troublesome preparation of materials required by some, high cost, and low sensitivity and low specificity of some methods. Therefore, it is very difficult for the majority of aquaculture farmers to master key technologies Promotion, limiting the application and development of these technologies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rockfish viral nerve necrosis virogene diagnostic kit and detecting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: Gene diagnostic kit of grouper viral neuronecrosis virus

[0041] 1. RNA extraction solution (solution A), 2 tubes, 5ml / tube, filled with Trizol solution.

[0042] 2. Solution B, self-prepared, mainly chloroform, 200μl / part x 10 parts, 2ml in total.

[0043] 3. Solution C, self-prepared, mainly isoamyl alcohol, 500μl / part x 10 parts, 5ml in total.

[0044] 4. Liquid D, self-prepared, mainly 70% ethanol, 1ml / part x 10 parts, 10ml in total.

[0045] 5. Liquid E, 1 tube, 0.5ml / tube, filled with DEPC water.

[0046] 6. RT reaction solution (solution F), 1 tube, 0.1ml / tube, containing RT reaction solution (10μl system), including 5×Buffer, dNTP, DEPC-H 2 O.

[0047] Random primers, RNase inhibitor RNasin (RI), reverse transcriptase M-MLV (RT).

[0048] 7. RT-PCR reaction solution (solution G), 1 tube, 0.5ml / tube, containing RT-PCR reaction solution (50μl system), including 10×Buffer (containing Mg 2+ ), dNTP, forward primer, reverse primer, ddH 2 O and T...

Embodiment 2

[0069] Embodiment 2: detection method of grouper viral neuronecrosis virus

[0070] Use the test kit of embodiment 1, carry out according to the following steps:

[0071] 1. Take 0.1 g of the sample and add 1 ml of liquid A to dilute, homogenize in an ice bath in a homogenizer, and let stand at room temperature for 3 to 5 minutes.

[0072] 2. Add 200 μl of solution B to the above homogenate, mix by inverting up and down, and let stand for 5 minutes.

[0073] Centrifuge at 12000r / min for 15min at 3.4°C.

[0074] 4. Take 400 μl supernatant, add it to 500 μl solution C, shake gently, and let it stand for 10 minutes.

[0075] 5.4°C, centrifuge at 12000r / min for 15min.

[0076] 6. Discard the supernatant, wash twice with 1ml pre-cooled solution D, and centrifuge at 7500r / min at 4°C for 5min.

[0077] 7. Air-dry or blow-dry on an ultra-clean workbench, add 50 μl E liquid water to dissolve (if it cannot be completely dissolved, it can be placed at 55-60°C for 10 minutes). The re...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention is rockfish viral nervous virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of rockfish viral nervous virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of tiger frog virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of rockfish cultivation and environment monitoring.

Description

technical field [0001] The invention relates to a diagnostic kit and a detection method for diseases of aquatic economic animals, and mainly relates to a kit and a detection method for gene diagnosis of grouper viral nerve necrosis virus (VNNV, Viral Nervous Necrosis Virus). Background technique [0002] Grouper is one of the famous and valuable marine fishes in the world. Because of its fast growth and high and stable market price, it is very popular among farmers. It is a traditional marine fish in southern my country. In recent years, with the rapid development of my country's marine aquaculture industry, the scale of grouper culture in cages has also continued to expand. Due to the increase in the number of cages in the breeding sea area and the increase in the breeding density, the environment of the breeding waters is gradually deteriorating, and the occurrence of diseases is becoming more and more frequent. Common diseases include parasitic diseases, bacterial diseas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 何建国黄剑南黄志坚邓敏吕玲陈晓艳翁少萍
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com