Three-in-one seven-fold PCR detection primer set, kit and detection method for virulence genes of Streptococcus agalactiae

A technology of virulence gene and Streptococcus lactis, which is applied in the field of triplex seven-fold PCR detection primer set, can solve the problems of indetermining the source and typing of the host of Streptococcus agalactiae, as well as the variation and deletion of virulence gene spectrum, and achieve saving Cost and time, rapid pathogen detection, and the effect of reducing labor costs
CN108707680BActive Publication Date: 2021-12-24SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2021-12-24

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Abstract

The invention discloses a triplex seven-fold PCR detection primer set for virulence genes of Streptococcus agalactiae, including virulence genes sip, fbsA, hylB, cfb, sodA, DltR, cspA, ponA, bibA, srr-1, bca, PCR amplification primer pairs corresponding to iagA, scpB, fbsB, pavA, psaA, spb1, bac, cppA, lmb and cylE. The sequences of each primer are shown in the sequence table. The invention also discloses a reagent kit comprising the above primer set and a three-in-one seven-fold PCR detection method of the Streptococcus agalactiae virulence gene using the above primer set. The detection method uses sip, fbsA, hylB, cfb, sodA, DltR and cspA as the first group, ponA, bibA, srr-1, bca, iagA, scpB and fbsB as the second group, pavA, psaA, spb1, bac , cppA, lmb and cylE are the third group, and PCR reaction is carried out under the same reaction conditions at the same time, and the target sequences of 21 virulence gene target fragments are amplified at the same time. Source and typing of Streptococcus lactis host, and evaluate the variation of strain virulence gene profile.
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Description

technical field

[0001] The invention relates to the detection of Streptococcus agalactiae, in particular to a primer set, a kit and a detection method for three-link seven-fold PCR detection of virulence genes of Streptococcus agalactiae. Background technique

[0002] Streptococcus agalactiae (Streptococcus agalactiae) belongs to Bacillus class, Lactobacillus order, Streptococcus family, Streptococcus genus, also known as group B streptococci (GBS), is a Gram-positive coccus that causes neonatal It is an important pathogenic bacterium of infantile meningitis, septicemia, pneumonia and cow mastitis, which can infect a variety of aquatic animals. At present, more than 80 kinds of farmed fishes are found to be susceptible to Streptococcus agalactiae infection, and the loss caused by Streptococcus agalactiae in the world's aquaculture industry is as high as 10 billion US dollars every year.

[0003] The molecular serotypes and ST types of Streptococcus agalactiae derived from h...

Claims

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