278 results about "Streptococcus lactis" patented technology

The invention discloses a shrimp preservative which comprises the following basic components: 0.5-50g/L of pulullan polysaccharide, 0-1g/L of natamycin, 0-5g/L of nisin from streptococcus lactis, 0-20g/L of ascorbic acid, 0-10g/L of polyglutamic acid, 0-20g/L of other antioxidants, 0-30g/L of other antibacterial components, 0-20g/L of other gum components and water as a solvent. When shrimps are soaked, sprayed and/or rubbed with the preservative, the refreshing time of the treated shrimps can be effectively prolonged for 3-4 days. The preservative has the advantages of good filming and preservation property, high security, high antibacterial and anti-oxidation efficiency, long action time and the like.

The invention relates to a biosynthesized gamma-aminobutyric acid preparation. By weight percentage, the product comprises 5 percent to 60 percent of gamma-aminobutyric acid. The preparation method of the biosynthesized gamma-aminobutyric acid preparation includes the following steps: first, streptococcus lactis strains are inoculated to a seed medium for fermentation composed of glucose, corn steep powder, defatted soybean flour and monosodium glutamate, thus forming fermentation broth; the fermentation broth is drained into a high speed centrifuge to form clear liquid centrifugally; the clear liquid is added with 250 mg/L chitosan at the temperature of 40 DEG C, and stirred for flocculation; the flocculated fermentation broth to be filtered enters a plate-and-frame filter, thus obtaining filtered clear liquid; the filtered clear liquid passes a cationic resin exchange bed for ion exchange; when ion exchange resins are saturated, the liquid is eluted by deionized water to elute all the glutamate; ammonia is used for eluting the liquid and extracting the gamma-aminobutyric acid. The method realizes large scale production of functional food factor GABA, so the method adapts to the production and processing of food with various forms and specifications as well as GABA products of nutritional foods.

The invention discloses a microbial inoculum for improving water body and structure of biological intestinal colony, and a preparation method and application thereof. In the method, bdellovibrio bacteriovorus., photosynthetic bacteria, streptococcus lactis, Lactobacillus bulgaricus and aquaculture biological intestinal beneficial strains are mixed to obtain the microbial inoculum for improving water body and structure of biological intestinal colony. The modified microbial inoculum is applied in fresh water aquaculture process, thereby effectively improving aquaculture environment and the structure of biological intestinal colony. The tropism and splitting capability of bdellovibrio bacteriovorus are utilized in the invention, and simultaneously the vacant niche left by pathogenic bacterium can be filled by the bacteria and other good bacteria which can be colonized in the intestinal canals of cultured organisms, thereby realizing the purpose of improving aquaculture environment and the structure of biological intestinal colony. The microbial inoculum of the invention has effectiveness, environment protection and no generation of pathogenic bacteria resistance, thereby having good application prospect.

The invention relates to a method for preparing the microbial fermentation feed for improving pork quality, which is characterized by comprising the following steps: preparing compound strain culture medium; and inoculating the compound strain into the compound strain culture medium, wherein the compound strain is composed of bacillus licheniformis, bacillus subtillis, lactobacillus plantarum, streptococcus lactis and saccharomyces cerevisiae; culturing to obtain compound bacteria liquid; mixing the compound bacteria liquid, bean pulp, wheat bran, corn, molasses and water; packaging; and carrying out anaerobic fermentation to obtain the microbial fermentation feed. The method of the invention has the advantages that the feed is easy to be absorbed and utilized by compounding the fermentation of the beneficial microorganisms, the commercial pig growth speed is higher, the slaughter rate is high; the muscle drip loss is lowered, and the total contents of the lean meat percentage, the cooked meat percentage, the muscle crude protein and the amino acid are enhanced.

The invention provides a method for producing nisin by continuous fermentation, which is characterized by comprising the following steps: transferring streptococcus lactis which is subjected to primary seed cultivation and secondary seed cultivation to a sterile fermentation medium, and controlling the temperature and pH value in the fermentation process; and when the bacterial concentration reaches a specified level, starting biosynthesis of the nisin, releasing fermentation broth from a fermentation tank continuously while replenishing a well-prepared sterile feed supplement medium in the fermentation tank continuously, and lasting for 7 to 15 days for the continuous production technology. In the invention, Lactococcuslactis of the streptococcus lactis is adopted to be subjected to two-stage seed cultivation, a specified fermentation medium formula is used, the feed supplement speed, the pH value and the bacterial concentration in a bioreactor are controlled, and the fermentation broth containing the nisin is released continuously or intermittently, which is used for producing the nisin. The method of the invention can effectively lessen non-optimal economic stages in the fermentation process, thereby realizing the purposes of improvement of fermentation yield, enhancement of device availability, and reduction of production cost for products.

The invention relates to immunogenic polysaccharide-protein conjugates comprising a capsular polysaccharide (CP) from Streptococcus agalactiae, commonly referred to as group B streptococcus (GBS), and a carrier protein, wherein the CP is selected from the group consisting of serotypes Ia, Ib, II, III, IV, V, VI, VII, VIII, and IX, and wherein the CP has a sialic acid level of greater than about 60%. The invention also relates to methods of making the conjugates and immunogenic compositions comprising the conjugates. The invention also relates to immunogenic compositions comprising polysaccharide-protein conjugates, wherein the conjugates comprise a CP from GBS serotype IV and at least one additional serotype. The invention further relates to methods for inducing an immune response in subjects against GBS and/or for reducing or preventing invasive GBS disease in subjects using the compositions disclosed herein. The resulting antibodies can be used to treat or prevent GBS infection via passive immunotherapy.

The invention discloses the purification technology of streptococcus lactis. The method comprises the ion exchange and backward high efficiency liquid chromatography. The recovery ratio is above 50%, and the purity displayed by HPLC is 98%.

An deactivated multi-bacterium vaccine for preventing the mastitis of milk cow is composed of Staphylococcus aureus, agalactic streptococcus, milk-stopping streptococcus, breast streptococcus, colibacillus and aluminum hydroxide gel. Its preparing process includes such steps as choosing the pathogenic bacterial strain of said mastitis, preparing seed liquid and reproduction liquid, purity checking, deactivating, preparing vaccine, and discriminating.

A biological preservation method for iced chicken meat comprises the steps that fresh chicken carcasses obtained after slaughter and cleaning are soaked in a preservative for 1-3 minutes and are taken out to undergo draining-off for 3-5 minutes, then the chicken carcasses are packed by using packing pieces, vacuum pumping is performed, and finally the chicken carcasses are preserved at low temperature. The preservative is an aqueous solution prepared by blending nisin from streptococcus lactis, lysozyme and chitosan, wherein the nisin from streptococcus lactis accounts for 0.03-0.05% by mass, the lysozyme accounts for 0.01-0.03% by mass and the chitosan accounts for 0.2-0.5% by mass in the preservative. The biological preservation method can remarkably prolong the preservation period of iced fresh chickens and can well keep the nutrition and flavor of iced chicken meat. In addition, after the iced chicken meat are taken out and unfrozen within the preservation period, it can be guaranteed that the meat is not slightly stiff and does not have sticky feel, and the preservation effect is greatly improved.

The invention discloses a microbial additive for alfalfa haylage, a preparation method and an application thereof. The additive is prepared from a bacterial suspension of aspergillusoryzae and an MRS liquid nutrient medium for streptococcus lactis according to the ratio of 50-70%: 50-30% in weight percent, wherein per milliliter of the bacterial suspension of the aspergillusoryzae at least contains 1.0*108 aspergillusoryzae spores, and per milliliter of the MRS liquid nutrient medium at least contains 4.0*108 cfu streptococcus lactis. The microbial additive quickens the initial fermentation rate of lactobacillus, enhances acid production capability, effectively inhibits the activity of saccharomycetes and other undesirable microorganism, strengths haylage effect and effectively promotes the production performance of cows, beef cattle, mutton sheep and swine. The strain adopted by the invention can be obtained easily, can be produced massively on application and has practicability.

The invention discloses a method for raising phasianus colchicas by using napier grass and relates to the field of chicken culture. The raising is divided into three stages, i.e., chickling raising, growing chicken raising and grown chicken raising, a feed formula adopted during the raising of each stage comprises a concentrated feed, fermented napier grass and a premix, proportioning ratios of the concentrated feed to the fermented napier grass to the premix in the three stages are all different, and the proportion of the fermented napier grass gradually increases; the fermented napier grass is prepared through fermenting 4 kinds of microbes, i.e., streptomyces, white-rot fungi, Lactobacillus acidophilus and Streptococcus lactis with peptone, molasses, calcium chloride, potassium sulfate, magnesium sulfate and citric acid jointly. According to the method for raising the phasianus colchicas, disclosed by the invention, the quality of fermentation of the napier grass is improved, and the quality of chicken is further improved; the raising ratio of the concentrated feed to forage is reasonably prepared according to growth characteristics of the phasianus colchicas, so that the aims of saving the concentrated feed, reducing the cost and improving the economic benefit are achieved.

The invention relates to an enzyme for conditioning a spleen and stomach damp-heat constitution and a preparation method of the enzyme. The enzyme comprises following raw material components: A1, 10% of almonds, 10% of black sesames and 15% of semen coicis; A2, 15% of peas, 15% of kidney beans, 3% of capillary artemisia, 3% of chrysanthemums, 10% of wax gourds, 10% of sechium edule, 3% of alisma plantago-aquatica, 3% of medlar and 3% of glycyrrhiza uralensis; probiotics is composed of C1: monascus, rhodotorula gracitis and torulopsis candida, C2: brettanomyces, streptomyces globisporus and streptomyces melanochromogenes, and C: kluyveromyces lactis and streptococcus lactis. The preparation method comprises the following steps: 1, immersing the A1 in the raw materials and cooking; inoculating the C1 to ferment for 7 days; inoculating the C2 to ferment for 7 days; 2, crushing the A2 materials and inoculating the C1 and the C2 to ferment for 14 days; and 3, fermenting the 1 and the 2 and mixing in a ratio of 1 to 1; and inoculating the C3 to ferment for 90 days to obtain a suspension solution, namely the enzyme for conditioning the spleen and stomach damp-heat constitution. The enzyme for conditioning the spleen and stomach damp-heat constitution has the effects of removing heat and draining dampness, and tonifying spleen and stomach.

The invention discloses lactococcus lactis KUST48. The preservation number of the lactococcus lactis KUST48 in China General Microbiological Culture Collection Center is CGMCC NO. 20699. The strain isapplied to treatment of zebra fish infected by streptococcus agalactiae, and experimental results show that the strain can significantly inhibit propagation of streptococcus agalactiae in intestinaltracts of zebra fish and reduce the death rate of zebra fish infected by streptococcus agalactiae. The lactococcus lactis aims to replace antibiotics, overcome a series of defects of excessive use ofantibiotics, drug residue, drug resistance, food safety, environmental pollution and the like, improve the immune function of aquatic animals, enhance the disease resistance of the aquatic animals, reduce the use amount of drugs and promote green and ecological development of the aquaculture industry.

The invention discloses a phage flocculant and application of the phage flocculant in a fermentation post-treatment technology and belongs to the field of a microbial post-treatment technology. The phage flocculant is characterized in that the phage flocculant is a cationic flocculant, and a working concentration of the phage flocculant is 0.1-5% generally. The flocculant is applied in the fermentation post-treatment technology of a vibrio parahaemolyticus phage, a coliphage, a salmonella phage, a xanthomonas axonopodis phage, a ralstonia solanacearum phage, a staphylococcus aureus phage, a streptococcus agalactiae phage, a streptococcus iniae phage or a pseudomonas aeruginosa phage. The phage flocculant can play a role of rapid subsidence of bacteria, be convenient and quick to operate, reduce centrifugal equipment investment and manpower and material resource loss and meet requirements of industrial mass production of the phage, and is safe, nontoxic and harmless to animals and plants.

The invention provides a microorganism strain- Streptococcus lactis 2013-DP1 which can ferment and utilize soybean meal and generate Nisin, and applications in preparation of feed additives through microbial fermentation. The strain is preserved in China Center for Type Culture Collection. The address is Wuhan University, Wuhan, China. The postcode is 430072. The preservation date is June 21th, 2013. The preservation number is CCTCC No: M2013275. By utilization of the strain, fermentation treatment of feed raw materials of soybean meal and the like is carried out. Contents of proteins and soluble small peptides (acid-soluble proteins) in feed raw materials can be raised, and Nisin with high activity is generated. The strain is used for the feed industry, and has great market potential.

The invention discloses an LAMP primer combination for detecting 8 environmental pathogens of dairy cow mastitis and application thereof. The primer combination provided by the invention is composed of 48 primers shown in sequence 1 to sequence 48. The primer combination provided by the invention can be used for detecting whether a to-be-detected bacterium is Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus dysgalactiae, Streptococcus uberis, Salmonella typhimurium, Proteus mirabilis or Candida albicans, and can be used for detecting whether a to-be-detected sample contains Escherichia coli and/or Klebsiella pneumonia and/or Pseudomonas aeruginosa and/or Streptococcus dysgalactiae and/or Streptococcus uberis and/or Salmonella typhimurium and/or Proteus mirabilis and/or Candida albicans. The primer combination provided by the invention is used for identifying and detecting the 8 environmental pathogens of dairy cow mastitis, has high specificity and high sensitivity, and can realize simple, rapid and accurate detection, thus having great popularization value.