Microbial ensiling strain and composite fungus, method for producing silage

A technology of silage and microorganisms, applied in the field of microbial feed additives, to achieve the effect of rich nutrition and waste reduction

Inactive Publication Date: 2004-09-22
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI +1
View PDF7 Cites 41 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the problems currently existing, the object of the present invention is to provide a kind of microbial silage bacterial strain and composite bacteria and its method for producing silage, through efficient selection of Streptococcus lactis and Lactobacillus plantarum, Bacillus subtilis and utilin Three strains of Candida constitute a highly active composite microbial additive. The invention effectively solves the technical problems in the fermentation of silage, and at the same time is convenient for farmers, simple and practical

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial ensiling strain and composite fungus, method for producing silage
  • Microbial ensiling strain and composite fungus, method for producing silage
  • Microbial ensiling strain and composite fungus, method for producing silage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment one: Streptococcus lactis and its function in silage strains

[0051] Lactococcus, Streptococcus lactis, facultative anaerobic lactic acid producing bacteria. In the initial stage of silage, it can multiply rapidly, produce a large amount of lactic acid, quickly reduce the pH, and effectively control the silage process.

[0052] 1.1 Strain culture

[0053] Plate medium: corn steep liquor 3%, soluble starch 2.3%, CaCO 3 2%, agar 2%, pH6.8-7.0, 1.6ml of 1.6% bromocresol phenol purple ethanol solution, sterilized at 121°C for 30 minutes;

[0054] Slant culture medium: do not add bromocresol phenol purple ethanol solution, and the rest of the ingredients are the same as above to make a slant (first subpackage and then sterilize, or first sterilize and then subpackage).

[0055] Activation of strains: draw a line on the plate from the slant strains or fermentation broth, culture at 37°C for 24 hours to 48 hours, and obtain a single colony (the surrounding cultu...

Embodiment 2

[0098] Microbial silage complex is a mixture of: 2 grams of Candida utilis, 1 gram of Streptococcus lactis, 1 gram of Lactobacillus plantarum, and 1 gram of Bacillus subtilis composition.

[0099] The method for producing livestock feed by fermenting silage stalks with the above-mentioned microbial silage compound bacteria is as follows:

[0100] 1. Raw material preparation:

[0101] (1) 2.5 tons of whole corn (cut at wax ripening stage), chopped to 2cm-3cm;

[0102] (2) 150 kg of water, add 1.2 kg of salt to prepare 0.8% saline solution;

[0103] (3) 15 kg of table salt.

[0104] 2. Bacteria resurrection: use 5 grams of silage agent. Put the silage bacteria into 500ml of clear water, add 4g-5 white granulated sugar, revive it after about 0.5 hours at room temperature, and then pour the bacteria agent into the pre-alkaline salt water to dissolve. The revived bacterial solution should be used as it is prepared, and it is best to use it up on the same day.

[0105] 3. Cell...

Embodiment 3

[0109] The microbial silage complex consists of: 2 grams of Candida utilis, 1.5 grams of Streptococcus lactis, 1 gram of Lactobacillus plantarum, and 0.5 grams of Bacillus subtilis.

[0110] The method for producing livestock feed with the above-mentioned microbial silage compound bacterial agent is the same as in Example two.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
decomposition efficiencyaaaaaaaaaa
Login to view more

Abstract

A high-activity composite microbial product for ensilage is composed of the streptococcus lactis and other 3 bacterial strains. After it is added to the raw materials of ensilage, such as corn plant, alfalfa, straw, stalk and pasturage, their pH value can be quickly lowered and the cellulose and semi-cellulose can be decomposed to obtain excellent feed.

Description

Technical field: [0001] The invention relates to a strain and a compound strain thereof in the field of microbial feed additives, in particular to Streptococcus lactis and its compound strains with other strains used in microbial feed additives in silage. [0002] The invention also relates to a method for producing silage feed using microbial silage compound bacteria compatible with lactobacillus and other strains. Background technique: [0003] my country's crop straw resources rank first in the world, with an annual output of 600 million tons of straw and about 570 million tons of green roughage. Among them, 42% are returned to the field directly or through the abdomen, 30% are used as agricultural fuel, 8% are used for industrial or other purposes, and 20% of about 120 million tons are not used, resulting in a great waste of potential feed resources. [0004] In order to prevent the destruction and waste of various resources in the process of agricultural production, re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A23K30/18C12N1/00C12N1/20
Inventor 王炜崔卫东魏东马彩霞张慧涛侯新强龙涛常玮阎论
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap