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298results about How to "Raise the threshold" patented technology

Method and device for detecting copy number variation based on amplicon next generation sequencing

The invention discloses a method and device for detecting copy number variation based on amplicon next generation sequencing. The method comprises the following steps: S1, extracting DNAs (Deoxyribonucleic Acid) of a sample to be detected and a negative comparison sample respectively and amplifying a target region with relatively mutated target characters through a plurality of amplicons, wherein the negative comparison sample is a sample with target character related genes which are not mutated; S2, carrying out next generation sequencing to obtain a sequence of the target region; S3, comparing the sequence of the target region and a reference genome, and carrying out data information processing to obtain a coverage degree of each amplicon; and S4, establishing a normal distribution model of the negative comparison sample on the coverage degree of each amplicon respectively and taking the normal distribution model as background noises, carrying out bilateral checking on the coverage degree of each amplicon of the sample to be detected and the normal distribution model, and taking amplicons which do not belong to normal distribution as copy number variation and positively detecting. By adopting the technical scheme provided by the invention, the accuracy of copy number variation detection based on the amplicon next generation sequencing is improved.
Owner:天津诺禾致源生物信息科技有限公司

Glycerol monolaurate microcapsule as well as preparation method and application of glycerol monolaurate microcapsule

The invention discloses a glycerol monolaurate microcapsule as well as a preparation method and an application of the glycerol monolaurate microcapsule. The preparation method comprises the following steps that: the glycerol monolaurate and emulsifying agents are dissolved into water being 60 to 70 DEG C to obtain solution A; wall materials are dissolved into the water being 60 to 70 DEG C to obtain solution B; the solution A and the solution B are mixed when the solution A and the solution B are still hot, stirring is carried out, and emulsion is obtained; and the emulsion is subjected to homogenizing and spray drying to obtain the glycerol monolaurate microcapsule, wherein the emulsifying agents are at least one kind of materials from sucrose ester, granulesten and fatty acid monoglyceride; and the wall materials are a mixture of maltodextrin and high-molecular compounds. The method provided by the invention is simple and practical, the controllability is strong, and the microencapsulation efficiency on core materials is higher; and the prepared glycerol monolaurate microcapsule has good flowability, dissolution dispersibility and mouth feeling, in addition, the anti-bacterium effect is not obviously reduced, and the glycerol monolaurate microcapsule can be applied to infant formula powder and realizes the effects of enhancing the immunity and resisting the infection.
Owner:ZHEJIANG UNIV +1

Square-wave pulse laser device and square-wave pulse laser generating method

The invention provides a square-wave pulse laser device, which comprises a nonlinear amplification environment NALM and a pulse laser main oscillation cavity, wherein the nonlinear amplification environment NALM is used for performing intra-cavity pulse shaping on laser pulse; and the pulse laser main oscillation cavity is used for circularly oscillating a square-wave laser pulse sequence in the nonlinear amplification environment NALM and the pulse laser main oscillation cavity and generating and outputting square-wave laser pulse. The invention also provides a square-wave pulse laser generating method, which comprises the following steps of: inputting continuous laser to excite a working substance to generate primary laser pulse; and performing intra-cavity pulse shaping on the primary laser pulse through the nonlinear amplification environment NALM, and circularly oscillating the primary laser pulse in the nonlinear amplification environment NALM and the pulse laser main oscillation cavity to generate the square-wave laser pulse. According to the pulse laser device and the method provided by the embodiment of the invention, the pulse laser device can directly output the square-wave laser pulse and has the characteristics of self start and simple adjustment.
Owner:UNIV OF SCI & TECH OF CHINA

Method for dynamic calculation of noise levels

The invention relates to a method for the dynamic measurement of noise level, in the method, the threshold value (12) is adapted to a signal output of a sensor by means of an echo procedure, and particularly the distance measurement is carried out by means of an ultrasonic procedure, wherein, a transmission signal (2) of the radiated object is sent back to the sensor in form of an echo signal (5), wherein, the time distance between sending of the transmission signal and the reception of the echo signal is measured, wherein, between the time of the decay of the transmission signal and the time of the arrival of the first echo signal (5), a time interval (6) is measured, and a threshold value (13) in the time interval (6) is gradually changed, wherein, the input noise signal is monitored according to compare of current threshold value (13), wherein, the current threshold value (13) becomes lowered, so long the input noise signal is below the current threshold value, and wherein, the current threshold value (13) becomes increased, so long the input noise signal is above the current threshold value (13), wherein, the current threshold value (13) becomes registered within the time interval (6) and wherein from the registered threshold values (13) a threshold value line (12) is determined.
Owner:ROBERT BOSCH GMBH

Manufacturing method of crystal grapefruit

The invention relates to a manufacturing method of a crystal grapefruit, which aims at solving the problem that the grapefruit peel has strong bitterness and is inedible. The manufacturing method comprises the following steps: using microwaves to debitterize under the alkaline condition, processing with sugary ingredients of sucrose and fructose dextrose syrup, embedding with beta-cyclodextrin molecules to further debitterize, and finally adding gelata to prepare into a spongy transparent food which contains the grapefruit peel and is soft in texture. The invention has the following advantages: instead of the method of completely removing the bitter substances in the grapefruit, the reasonable recipe design and process conditions are adopted, especially the microwave debitterizing, the processing with sugary ingredients and the beta-cyclodextrin embedding processes, decreases the bitter substances of the grapefruit peel, effectively raises the threshold value of the bitterness, and enables the product to have favorable flavor and texture and better preserve the nutritious substances and the biological active substances of the grapefruit. The gellan gum added to the gelata can enhance the elasticity of the product and improve the transparency of the product; and what's important, the gelata is very stable under the acidic condition.
Owner:HUBEI UNIV OF TECH
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