Strain of Streptococcus lactis and applications in preparation of feed additives through microbial fermentation

A technology of fermentation of Streptococcus lactis and microorganisms, applied in the direction of microorganism-based methods, microorganisms, animal feed, etc., can solve the problems of not being able to exert potential functions, achieve the effects of improving intestinal health, reducing large-scale use, and improving nutritional quality

Active Publication Date: 2014-03-19
HUBEI XIPU BIOTECH
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Problems solved by technology

These protein-containing wastes are often simply disposed

Method used

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  • Strain of Streptococcus lactis and applications in preparation of feed additives through microbial fermentation
  • Strain of Streptococcus lactis and applications in preparation of feed additives through microbial fermentation
  • Strain of Streptococcus lactis and applications in preparation of feed additives through microbial fermentation

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Embodiment 1

[0021](1) Isolation, culture and screening method of Streptococcus lactis: spread Streptococcus lactis on a solid detection medium plate containing indicator bacteria after a certain dilution, after culturing at 30°C for 24 hours, measure the diameter of the inhibition zone, and pick out the antibacterial bacteria. The titer of producing Nisin was measured after storing the strain with a large circle diameter to the colony diameter. Nisin has a strong antibacterial effect on Gram-positive bacteria. Staphylococcus aureus was selected as the indicator bacterium, and the Nisin produced by Streptococcus lactis had an antibacterial effect on it, making it produce an inhibition zone. The diameter of the bacterial circle was used to screen the better strains and evaluate their potency.

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Abstract

The invention provides a microorganism strain- Streptococcus lactis 2013-DP1 which can ferment and utilize soybean meal and generate Nisin, and applications in preparation of feed additives through microbial fermentation. The strain is preserved in China Center for Type Culture Collection. The address is Wuhan University, Wuhan, China. The postcode is 430072. The preservation date is June 21th, 2013. The preservation number is CCTCC No: M2013275. By utilization of the strain, fermentation treatment of feed raw materials of soybean meal and the like is carried out. Contents of proteins and soluble small peptides (acid-soluble proteins) in feed raw materials can be raised, and Nisin with high activity is generated. The strain is used for the feed industry, and has great market potential.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain obtained through screening and mutagenesis——Streptococcus lactis (Streptococcus lactis) 2013-DP1, and its application in the preparation of feed additives by microbial fermentation. (2) Background technology [0002] In the feed industry and related products, antibiotics are often added to the feed for the prevention and treatment of livestock and poultry diseases. With the widespread use of antibiotics in feed and people's higher and higher requirements for food quality, the side effects, potential hazards and risks of antibiotics have been increasingly exposed. The long-term additive use of antibiotics can cause many pathogenic bacteria to develop drug resistance. At the same time, serious drug residues are produced in animal products, which has a serious adverse effect on human health. The application of antibiotics in the feed industry is facing elimination. or disabled situation. It has become ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/02A23K1/17A23K1/14C12R1/46A23K20/195
Inventor 朱廷恒汪琨崔志峰吴斌王渭霞
Owner HUBEI XIPU BIOTECH
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