Cow mastitis concatenate inactivated vaccine

A technology for dairy cow mastitis and inactivation of bacteria, applied in the directions of antibacterial drugs, bacterial antigen components, sexual diseases, etc. The effect of high added value, broad market application prospect and low production cost

Inactive Publication Date: 2007-01-17
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When mastitis occurs, although there are many cases of mammary gland swelling and pain, most cases of recessive cow mastitis are difficult to find abnormalities when palpated by hand, and pathological changes are also difficult to observe with naked eyes
[0003] At present, the prevention and treatment of mastitis in dairy cows is still mainly based on antibiotic treatment and environmental sanitation control, but antibiotic treatment is often ineffective due to the emergence of drug resistance, resulting in serious antibiotic residues in milk and endangering human health.
In response to this problem, domestic and foreign in recent years have begun to study and use bacterin immunization methods to prevent and control this disease, and have received certain results. For example, Ze'

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] Staphylococcus aureus, Streptococcus agalactiae, Streptococcus lactis and Streptococcus uberis were streaked and inoculated on agar medium containing 5% sheep blood, and Escherichia coli was streaked and inoculated on Eosin Methylene Blue and MacConkey medium, 37℃ Cultivate for 24h, observe the growth of the colony and use conventional biochemical methods to identify its characteristics.

[0025] The isolated strains were respectively inoculated on 10% sheep blood agar medium and cultured at 37°C for 24 hours. The pure cultures that passed the biological characteristics verification were Staphylococcus aureus, Streptococcus agalactiae, Streptococcus lactis and Streptococcus uberis They were respectively inoculated into appropriate amount of BHI liquid medium, and E. coli was inoculated into TSB liquid medium, cultured with shaking at 37°C for 24h, and stored at 4°C as seed solution. The seed solution was respectively inoculated into BHI and TSB liquid med...

Example Embodiment

[0028] Example 2

[0029] (1) Safety test

[0030] Ten standard mice of 21-25 days old were inoculated intraperitoneally with 1 mL vaccine, and 5 healthy rabbits were inoculated with 1 mL vaccine subcutaneously on the back and observed for 14 days. The results were normal and no abnormal symptoms were seen.

[0031] Choose healthy bred cows, normal lactating cows and pregnant cows, respectively, to inoculate the multi-linked inactivated vaccine obtained in Example 1, and each cowhide will be injected with 10 mL at points. Observe for 7 days and check the local and systemic responses of the inoculation. Results Only one bred cattle was swollen at the injection site and returned to normal in about 1 week; other vaccinated cattle had no obvious local and systemic reactions. The spirit, body temperature, appetite, breathing and pulse are normal, the milk production of the lactating cow is normal, and the fetus of the pregnant cow is normal without abortion.

[0032] (2) Immune efficac...

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PUM

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Abstract

An deactivated multi-bacterium vaccine for preventing the mastitis of milk cow is composed of Staphylococcus aureus, agalactic streptococcus, milk-stopping streptococcus, breast streptococcus, colibacillus and aluminum hydroxide gel. Its preparing process includes such steps as choosing the pathogenic bacterial strain of said mastitis, preparing seed liquid and reproduction liquid, purity checking, deactivating, preparing vaccine, and discriminating.

Description

technical field [0001] The invention relates to a veterinary biological product and a preparation method thereof, more specifically, to a multi-link inactivated bacterium vaccine for preventing cow mastitis and a preparation method thereof. Background technique [0002] Bovine mastitis (Bovine Mastitis) is the inflammation of the mammary gland tissue of dairy cows, which is mainly caused by the infection of pathogenic microorganisms in the mammary gland tissue. It is a common and frequently-occurring disease of dairy cows. According to clinical manifestations, it can be divided into recessive and clinical mastitis. There are about 150 kinds of pathogenic bacteria causing mastitis in cows, mainly Staphylococcus aureus, Streptococcus and Escherichia coli, accounting for more than 90% of the whole cow mastitis cases. Its main features are physical and chemical properties of milk and bacteriological changes, and pathological changes of mammary gland tissue. The most important ...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61P15/14A61P31/04
Inventor 崔玉东朱战波朴范泽
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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