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Oil emulsion vaccine for broilers and preparation method thereof

An oil emulsion and vaccine technology, which is applied in the field of Newcastle disease and H9 subtype avian influenza vaccines and their preparation, can solve problems such as unsatisfactory immune effect, and achieve the effects of short cycle, easy absorption and improved immune effect.

Inactive Publication Date: 2012-07-18
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the vaccines currently used for broiler chickens are similar to those used for laying hens, and the immune effect is not ideal

Method used

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  • Oil emulsion vaccine for broilers and preparation method thereof
  • Oil emulsion vaccine for broilers and preparation method thereof
  • Oil emulsion vaccine for broilers and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Newcastle disease-H for broilers 9 Preparation of subtype avian influenza dual inactivated vaccine

[0032] Preparation of oil phase: select high-quality white oil with a kinematic viscosity (40°C) of 7.64mm 2 / s. According to parts by weight, 94 parts of white oil and 806 parts of Span-806 are mixed, and 2 parts of aluminum stearate are added to prepare a transparent oily liquid, which is sterilized and cooled to room temperature.

[0033] Prepare the aqueous phase:

[0034] (1) Preparation of chicken Newcastle disease venom: Dilute 1,000 times the La Sota strain of virulent breeding chicken Newcastle disease virus used for production, inoculate susceptible chicken embryos, inoculate 0.1ml into the allantoic cavity of each embryo, incubate at 37°C, observe chicken embryos every day, for 60 hours The dead chick embryos were discarded. After that, observe once every 4-6 hours, and take out the dead chicken embryos at any time until 96 hours, regardless of whether the...

Embodiment 2

[0039] Newcastle disease-H for broilers 9 Preparation of subtype avian influenza dual inactivated vaccine

[0040] Preparation of oil phase: choose white oil, kinematic viscosity (40°C) is 4.29mm 2 / s, according to parts by weight, 94 parts of white oil, 806 parts of Span-806 parts, after mixing, add 2 parts of aluminum stearate to prepare a transparent oily liquid, sterilize, and cool to room temperature.

[0041] Prepare the aqueous phase:

[0042] (1) Preparation of chicken Newcastle disease venom: Dilute 1,000 times the La Sota strain of virulent breeding chicken Newcastle disease virus used for production, inoculate susceptible chicken embryos, inoculate 0.1ml into the allantoic cavity of each embryo, incubate at 37°C, observe chicken embryos every day, for 60 hours The dead chick embryos were discarded. After that, observe once every 4-6 hours, and take out the dead chicken embryos at any time until 96 hours, regardless of whether they are dead or not, take them all o...

Embodiment 3

[0047] Preparation of dual inactivated Newcastle disease-H9 subtype avian influenza vaccine for broiler chickens

[0048] Preparation of oil phase: choose white oil, kinematic viscosity (40°C) is 4.29mm 2 / s, according to parts by weight, 94 parts of white oil, 806 parts of Span-806 parts, after mixing, add 2 parts of aluminum stearate to prepare a transparent oily liquid, sterilize, and cool to room temperature.

[0049] Prepare the aqueous phase:

[0050] (1) Preparation of chicken Newcastle disease venom: Dilute 1,000 times the La Sota strain of virulent breeding chicken Newcastle disease virus used for production, inoculate susceptible chicken embryos, inoculate 0.1ml into the allantoic cavity of each embryo, incubate at 37°C, observe chicken embryos every day, for 60 hours The dead chick embryos were discarded. After that, observe once every 4-6 hours, take out the dead chicken embryos at any time until 96 hours, regardless of whether they are dead or not, take them all...

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Abstract

The invention discloses an oil emulsion vaccine for broilers and a preparation method thereof. The oil emulsion vaccine comprises a water phase and an oil phase, wherein the water phase contains concentrated and inactivated ND (newcastle disease) and H9 AI (avian influenza) venom and Tween-80; the oil phase contains an oil adjuvant formed by white oil, Span-80 and aluminium stearate; the volume ratio of the water phase to the oil phase is (1:1.6) to (1:2); and the kinematic viscosity (40 DEG C) of the selected white oil is 4-8mm<2> / s. The oil emulsion vaccine has the characteristics of easiness in absorption, quick effect and high level of the generated antibodies, is suitable for growth of the broilers, can effectively increase the immune levels of the broilers, can effectively protect the broilers from being invaded by ND and H9 AI viruses and avoids ND and H9 AI in the immune phase.

Description

technical field [0001] The invention relates to an oil emulsion vaccine suitable for broilers and a preparation method thereof, especially a kind of chicken Newcastle disease and H 9 Subtype bird flu vaccine and preparation method thereof. Background technique [0002] With the rapid development of the poultry farming industry, the occurrence of diseases also tends to be complicated. In order to pursue good production performance in modern broiler farming, there are more and more vaccines for chicken disease prevention, and more and more veterinary drugs to control chicken diseases, but there are still more and more chicken diseases. The characteristics of the disease of broiler chickens are that the mortality rate of broiler chickens is high. Once the disease occurs, the chickens will continue to die every day, and the course of the disease will last for a long time. Due to the unevenness of the breeding facilities and management levels, most diseases show obvious regional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K39/17A61K39/145A61K39/39A61P31/14A61P31/16
Inventor 刘建尤永君王永建陈利红杨旭
Owner TIANJIN RINGPU BIO TECH
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