The invention discloses eco-nutritional compound feed for laying hens at an egg-laying peak and a preparation method of the eco-nutritional compound feed. The eco-nutritional compound feed for the laying hens at the egg-laying peak comprises components in percentage by weight as follows: corn, bean pulp, rice bran, calcium hydrophosphate, rock flour, baking soda, sodium chloride, soybean oil, compound trace elements for the laying hens, compound vitamins for the laying hens, 50% choline chloride, a bacillus coagulans preparation, a fructo-oligosaccharide preparation, an DL-methionine, compound enzyme for the laying hens, phytase, montmorillonite and zeolite powder. The nutritional requirement of the laying hens can be met, the immunity and the anti-stress capacity of the laying hens can be effectively improved, the disease rate is reduced, the egg laying rate is increased, the lasting time of the peak is prolonged, the quality of eggs is improved, the environment of henhouses can be improved, and the eco-nutritional compound feed is a novel laying egg feed product integrating nutrition, the anti-disease property, promotion of a high yield and pollution-free property.