Inactivated lactic acid bacteria vaccine adjuvant
A technology for inactivating lactic acid bacteria and vaccine adjuvants, which is applied in the field of inactivated lactic acid bacteria vaccine adjuvants, and can solve the problems of high safety, efficacy to be improved, and high cost
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[0021] Example 1
[0022] Enterococcus faecium (purchased from China Industrial Microbial Culture Collection and Management Center, Latin name: Enterococcusfaecium, preservation number: CICC 6049) was inoculated into MRS medium, cultured in a 37°C incubator for 24 hours, and then centrifuged at 3000 rpm for 5 minutes to remove The upper culture medium retains the precipitate, adds sterile normal saline to wash the precipitate, centrifuges for 5 minutes, repeats the cleaning 3 times, adds sterile normal saline, and mixes the precipitate. Take a certain amount of Enterococcus faecium physiological salt suspension solution, measure its OD value at 690nm spectrophotometer, when the final concentration of OD value is 0.38 diluted with sterile normal saline, the concentration of Enterococcus faecium diluted in this way Physiological saline solution is regarded as 1 times (1×) concentration. After bacteria count, it can be determined that each ml of bacterial suspension contains ~10 8 A...
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[0023] Example 2
[0024] Dilute the inactivated Enterococcus faecium suspension prepared above to a concentration of 10×, and detect the effect of inactivated Enterococcus faecium as an adjuvant on the levels of antibodies to Newcastle disease and avian influenza H9 subtypes after chickens inoculated with the new dual inactivated vaccine . Twenty white feather broilers were randomly divided into 2 groups, namely the control group and the inactivated Enterococcus faecium adjuvant group, with 10 in each group. Vaccination was performed at 7 days of age. When the control group was vaccinated, 20 mL of sterile normal saline was added to 30 mL of the new-flow dual inactivated oil vaccine to mix well, and 0.5 mL was injected subcutaneously into the neck of each chicken; inactivated feces When the coccal adjuvant group was vaccinated, 20 mL of 10× concentration of inactivated Enterococcus faecium suspension was added to 30 mL of the new-flow dual inactivated oil vaccine to mix well, a...
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[0025] Example 3
[0026] Lactobacillus plantarum subsp. Plantarum (purchased from China Industrial Microbial Culture Collection and Management Center, Latin name: Lactobacillus plantarum subsp.Plantarum, preservation number: CICC 6240) was prepared into 10× concentration inactivated plants according to the method described in Example 1. Lactobacillus plant subspecies vaccine adjuvant. The effect of inactivated Lactobacillus plantarum subsp. plant as an adjuvant on the antibody level of swine fever vaccine in mice was tested. Twenty clean-grade Kunming mice weighing 18-22 g were randomly divided into 2 groups, namely the control group and the inactivated Lactobacillus plantarum subsp. plant adjuvant group, each with 10 mice, half male and half male. When mice in the control group were vaccinated with swine fever live vaccine, an equal volume of sterile normal saline was added to the swine fever vaccine; 100 μL was injected intramuscularly into the lateral hind limbs of the mice;...
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