109 results about "Duck embryo" patented technology

The invention relates to a method for preparing a duck virus hepatitis divalent refined egg yolk antibody. The method is that vaccines of a duck virus hepatitis virus 1 type YBH1 strain and novel YBHX strain with good immunogenicity are selected from duck virus hepatitis superior prevalent strains to prepare strains, the strains are respectively inoculated to a chicken embryo and a duck embryo, a dead embryo body and the embryo juice are respectively obtained, the virus liquid is collected after grinding and freeze thawing, ultrafiltration concentration and inactivation with formaldehyde solution are carried out, and oil adjuvant is added to mix and emulsify to produce the vaccine. The vaccine is inoculated to a laying hen, the egg with high immunity is obtained, the yolk is separated, inactivated, extracted and refined to produce the duck virus hepatitis divalent refined egg yolk antibody which can prevent the duck virus hepatitis which is caused by the 1 type and novel duck hepatitis viruses, so the egg yolk has the advantages of high efficiency, good safety, high protection ratio and the like.

The invention relates to preparation and application of an efficient cell inactivation vaccine against new duck reovirus disease. The preparation method comprises the following steps: an antigen preparation step: inoculating a virus NDRV-TH11 strain with immortalized duck embryo fibroblast to obtain cytotoxicity with 80% of cytopathic effect, and performing cryopreservation at -20 DEG C; and a vaccine preparation step: inactivating the obtained cytotoxicity and mixing with a homemade nano adjuvant and stirring uniformly to obtain the efficient cell inactivation vaccine against new duck reovirus disease. After the duck is immunized by the efficient cell inactivation vaccine against new duck reovirus disease prepared in the invention, the duck can effectively resist the attack of a virulent strain of the new duck reovirus.

The invention is concerned with a kind of preparation method of inactivated vaccine to prevent new castle disease, infectious bronchitis and egg drop syndrome vaccine. The selected genus is Newcastle Disease virus La Sota individual plant, infectious bronchitis virus M41 individual plant, HN99 kidney type of aberrance individual plant and EDS76 Jing 911 individual plant. Dilute three kinds of virus and inoculate in the allantoic cavity of SPF chick embryo and affectable duck embryo. Collect liquid of embryo to alive and dead embryos as seed. Dilute and inoculate in allantoic cavity of affectable chick embryo or duck embryo to get liquid of virus embryo. Concentrate with equipment and add with formaldehyde solution to prepare bacterin through emulsion process. This invention can prevent Newcastle Disease, chick kidney type and breathing type infectious bronchitis, and egg drop syndrome vaccine. It can reduce the times of injection and the cost of epidemic prevention for easy using and practicality.

The invention provides a bivalent egg yolk antibody against DVH (duck virus hepatitis) as well as a preparation method and an application of bivalent egg yolk antibody. The bivalent egg yolk antibody contains a DHAV (duck hepatitis A virus)-1 type egg yolk antibody against DVH and a DHAV-3 type egg yolk antibody against DVH. The preparation method comprises steps as follows: (1) a DHAV-1 type strain against DVH and a DHAV-3 type strain against DVH are inoculated with an SPF chick embryo and a susceptible duck embryo respectively, an allantoic fluid is obtained, obtained virus fluids are mixed in proportion and inactivated with formalin, and a vaccine is prepared; (2) laying hens are immunized with the vaccine, sampling is performed after immunization for measuring whether the neutralizing titer of DHAV-1 type and DHAV-3 type antigens and antibodies in hyperimmune egg yolk of chickens is larger than or equal to 1:8192, and later, hyperimmune eggs of the chickens are collected; (3) eggshells of the hyperimmune eggs are disinfected, isovolumetric distilled water is added after the egg yolk is collected, and the mixture is stirred and mixed uniformly and then is subjected to pasteurization at the low temperature; purification with an acidified distilled water method and purification with a caprylic acid method are performed; microfiltration and ultrafiltration are performed. The provided bivalent egg yolk antibody is low in cost and high in titer, DVH caused by DHAV-1 and DHAV-3 can be effectively controlled, and remarkable social benefits can be obtained.

The present invention provides a duck viral hepatitis bivalent yolk antibody and a preparation method thereof, wherein the bivalent yolk antibody comprises a duck viral hepatitis DHAV-1 type antibody and a duck viral hepatitis DHAV-3 type antibody. The preparation method comprises: (1) adopting a duck viral hepatitis DHAV-1 type strain and a duck viral hepatitis DHAV-3 type strain to respectively vaccinate SPF chicken embryo and susceptible duck embryo, harvesting allantoic fluid, mixing the harvested virus liquids according to a certain ratio, carrying out formaldehyde inactivation, and preparing a vaccine; (2) adopting the vaccine to immunize laying hens, sampling after immunization to determine whether the neutralizing titer of the anti-DHAV-1 type antigen antibody and the anti-DHAV-3 type antigen antibody in the chicken hyperimmune egg yolk is more than or equal to 1:8192, and collecting the hyperimmune egg of the chicken; and (3) disinfecting the eggshell of the hyperimmune egg, collecting the egg yolk, adding the equal volume of distilled water, uniformly stirring and mixing, carrying out low temperature pasteurization inactivation, adopting an acidification distilled water method to purify, adopting an octanoic acid method to purify, and carrying out micro-filtration and ultra-filtration. The duck viral hepatitis bivalent yolk antibody has characteristics of low cost and high titer, and can be provided for effectively controlling duck viral hepatitis caused by DHAV-1 and DHAV-3 so as to obtain significant social benefits.

The invention discloses a complex duck interferon-alpha gene, and a recombinant vector and application thereof. The nucleotide sequence of the complex duck interferon-alpha gene is disclosed as SEQ ID NO:1. The optimized complex duck interferon-alpha gene can be expressed in Pichia yeast gene engineering bacteria to produce complex duck interferon-alpha. The obtained complex duck interferon-alpha has the following advantages: high purity: the thin-layer chromatography scanning on the yeast-expressed duck interferon-alpha SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) result indicates that the target ribbons of the recombinant yeast duck interferon-alpha account for more than 80% of the total expression proteins; and high antivirus action: compared with the yeast-expressed natural duck interferon-alpha, the protein expressed by the novel genome has higher antivirus activity, and the action of resisting 100TCID50VSV virus infection on duck embryo fibroblasts is enhanced by 40 times. The complex duck interferon-alpha gene can be used for preparing drugs for treating poultry virus diseases.

The invention discloses a full suspension culture method of a duck plague virus, comprising the following steps: step 1: resuscitation and passage culture of passage cell lines derived from duck embryo cells; 2, inoculation of the duck plague virus into a passage cell line derived from a fully suspend culture duck embryo cell by adopting a second-order culture method to realize large-scale cultureof that duck plague virus. 3, after that virus is inoculated, the TCID50 of the virus is sampled and detected every 12 hours, the virus is harvested when the TCID50 of the virus reach the maximum, and the virus is stored to obtain the cultured duck plague virus. The full suspension culture method of the invention improves virus culture scale and efficiency, accords with the trend of vaccine production in the future, and has wide application prospect and good economic benefit.