18 results about "Duck embryo" patented technology

The invention discloses a duck-derived flavor peptide enrichment and identification method, and belongs to the technical field of food flavor and biology. The method comprises the following steps: performing after-ripening treatment on pickled duck embryos to enrich small peptides; performing extraction and ultrafiltration to obtain components less than 3kDa; carrying out LC-MS / MS identification on the sequence; using multiple machine learning models to crossly predict the palatable taste potential; carrying out molecular docking simulation on the candidate peptide and an umami receptor T1R1 / T1R3; and synthesizing and carrying out sensory verification. According to the method, six brand-new flavor peptides (SEQ ID NO: 1-6) are identified from after-ripe duck meat for the first time, the flavor threshold value of the six brand-new flavor peptides is 0.09-0.30 mmol / L, and the strength of the six brand-new flavor peptides is superior to that of monosodium glutamate. The method provided by the invention is efficient and accurate, and the obtained umami peptide is natural and safe and has important application value in the fields of food seasoning and flavor enhancement.

The utility model discloses a punching device for duck embryo inoculation, belongs to the technical field of punching devices, and solves the problems that the conventional device directly punches duck embryos on a duck embryo plate in a unified manner, and the normal development and experimental conclusion of the duck embryos are influenced by different punching depths due to different sizes of the duck embryos. A rotating device is arranged on the left side of the top face of the bottom plate, a punching assembly is arranged on the right side of the top face of the bottom plate, the rotating device comprises a first motor and a rotating disc, the first motor is fixedly installed on the left side of the bottom face of the bottom plate, and the output end of the first motor penetrates through the bottom face of the bottom plate and is fixedly installed on the bottom face of the rotating disc. Through cooperative use of the rotating device and the punching assembly, the distance between each punched duck embryo and the infrared distance meter can be measured, then the punching depth of the puncher is controlled, different punching depths can be controlled according to the duck embryos of different sizes, normal growth and development of the duck embryos are facilitated, and the accuracy of experimental results can be improved.

The invention belongs to the technical field of veterinary diagnosis, and particularly relates to a novel duck reovirus agar diffusion antigen as well as a preparation method and application thereof. The preparation method comprises the following steps: inoculating a novel duck reovirus GX2022 strain to an SPF (specific pathogen free) duck embryo at the age of 6-10 days, harvesting and inoculating a diseased duck embryo body within 24-144 hours, adding sterile normal saline, fully grinding, freezing and thawing (freezing at the temperature of-20 DEG C and thawing at the temperature of 25 DEG C), carrying out solid-liquid separation, collecting supernate, adding formaldehyde for inactivation, uniformly mixing the inactivated virus liquid with a freeze-drying protective additive, and freeze-drying to obtain the agar diffusion antigen. Compared with the prior art, the method has the advantages that the agar diffusion titer is as high as 1: 24, the specificity is strong, the stability is good, the titer is high, the yield is high, the reaction precipitation line is clear, the detection is convenient, the method can be used for evaluating the immune effect of the NDRV and diagnosing the infection of the NDRV, can also be used for screening NDRV antibody negative ducks, detecting exogenous virus antibodies of waterfowl live vaccines or virus seeds, performing epidemiological investigation and the like, and has a wide application prospect.

The application discloses a chimeric Kunjin virus of tamabi virus prM-E and a preparation method and application thereof. On the basis of a reverse genetic manipulation system of the Kunjin virus KUNV, the KUNV prM / E gene is replaced by the DTMUV prM / E gene to construct a chimeric DTMUV virus infectious clone plasmid with the KUNV as a skeleton, which is named as pUC19-KUNV-DTMUV prM / E. Then, the chimeric tamabi virus prM-E Kunjin virus is obtained through virus rescue, and is named as rKUNV-CHv-prM / E (CHv). Analysis on the virus characteristics shows that the CHv can effectively proliferate in BHK-21 and DEF cells, and the duck embryo virulence is weaker than that of the KUNV and the DTMUV. The animal experiment results show that the CHv only detects obvious virus levels in the brain, and does not produce viremia; the CHv after immunization can produce similar IgG antibody levels to those after immunization of the KUNV and the DTMUV. Therefore, the recombinant virus of the chimeric DTMUV prM / E with the KUNV as a skeleton prepared by the application can induce the duckling to produce sufficient immune protective neutralizing antibody levels, and can be applied to preparation of a duck tamabi virus vaccine.

According to the technical scheme, the auxiliary device for duck embryo injection is characterized by comprising a workbench, a fixing base is fixedly connected to one side of the workbench, a mechanical arm is fixedly connected to the top face of the fixing base, an injection part is arranged at one end of the mechanical arm, and a fixing part is arranged on the top face of the workbench; the duck embryo breathing state is accurately monitored, breathing vibration and gas metabolism characteristic indexes are obtained through analysis of the first monitoring unit and the second monitoring unit, then the breathing state is judged through the judgment unit, the injection instruction is sent out only when the duck embryo breathes stably, the mechanical arm drives the injector to complete vaccine injection, injection can be avoided when the duck embryo breathes rapidly in the process, and the vaccine injection efficiency is improved. The influence on the health of the patient is reduced, the injection effect is guaranteed, and the injection assisting effect is achieved.

The application discloses a recombinant novel goose parvovirus infectious clone plasmid and application, the infectious clone plasmid includes the full-length genome of novel goose parvovirus, and a synonymous mutation marker is introduced at the cysteine codon position in the non-structural protein coding region, under the condition that the amino acid sequence is not changed and the offspring virus replication and infection ability are not influenced, a stable single nucleotide genetic marker is provided for the novel goose parvovirus.The infectious clone plasmid can be used for rescuing the recombinant virus carrying a specific synonymous mutation marker, the obtained recombinant virus has good duck embryo adaptability and cell infection ability, and is suitable for application scenes such as cell infection and immunofluorescence detection.The application provides an important technical tool for novel goose parvovirus genetic marker tracking, pathogenic mechanism research, genetic variation analysis and vaccine research and development.

The invention relates to the technical field of microorganisms, in particular to a highly pathogenic duck virus hepatitis III virus and an egg yolk antibody, preparation and application thereof. The virus strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2024, the preservation number is CGMCC No.46271, and the virus strain is named as RD240820B. The egg yolk antibody is prepared by using the highly pathogenic duck virus hepatitis III type virus strain as an antigen. The egg yolk antibody is applied to preparation of products for preventing or treating duck virus hepatitis III. In a virulence experiment on a duck embryo, the death rate of the virus strain reaches 80% after the duck embryo is inoculated for 36 hours, and the virus strain has strong pathogenicity. And the death rate within 60 hours after inoculation is 100%, and the strain has relatively strong pathogenicity. The death rate is still 60% when the diluent is 10-8, and the toxicity is strong. The ELD50 value of the strain is relatively low, which indicates that the strain has relatively strong pathogenicity to duck embryos. After the egg yolk antibody is injected, the protection rate reaches up to 97.6% after the diseased duck is treated, and the egg yolk antibody has a remarkable prevention and control effect.

The invention belongs to the field of cell biology, and relates to a shelduck fibroblast cell line which is finally obtained by performing primary culture, screening and optimized subculture on shelduck embryos serving as materials. The fibroblasts cultured by the method are slender, fusiform, triangular and irregular, and can be continuously subcultured for at least 82 generations; the shelduck fibroblast cell line is used for culturing waterfowl viruses such as duck plague virus, novel duck reovirus, duck tembusu virus, duck parvovirus and the like for the first time; compared with the existing duck-derived fibroblast culture, the duck-derived fibroblast culture medium has the advantages of high virus content and wide application range. The shelduck fibroblast cell line provided by the invention can provide a large amount of high-quality materials for scientific researches of cell engineering, molecular biology, immunology and the like; the method is used for culturing, separating and identifying poultry viruses, provides raw materials for development and production of poultry attenuated vaccines and inactivated vaccines, can shorten the production period and reduce the production cost, and has certain practical significance for large-scale production of vaccines.

This invention discloses a highly active duck embryo liver primary cell and its low-cost preparation method and applications. The preparation method includes: obtaining and pretreatment of duck embryo liver tissue, separation by complex enzyme digestion, preparation of cell suspension, primary cell culture, and maintenance of cell viability. The core of this method lies in the gentle digestion using a complex digestion solution composed of collagenase I, trypsin, and DNase I in specific weight ratios, followed by culture in a well-defined, non-commercial growth factor-free complete culture medium. This complete culture medium contains synergistic components such as DMEM / F12, fetal bovine serum, epidermal growth factor, hepatocyte growth factor, and an insulin-transferrin-selenium mixture. The duck embryo liver primary cells prepared by this invention exhibit high yield, high viability, and excellent liver-specific function, and can maintain their activity and function well after passage and cryopreservation.

The invention belongs to the technical field of quadruple vaccines, and particularly relates to a quadruple vaccine and a preparation method thereof, the quadruple vaccine comprises virus seeds: a chicken Newcastle disease virus (NDV) Lasota strain, a chicken Newcastle disease virulent F48E8 strain, a chicken infectious bronchitis virus (IBV) M41 strain, a chicken egg drop syndrome virus (EDSV) EDS76-AV127 strain, and serum: ND, IB positive serum and negative serum. Test animals and chicken / duck embryos are non-immune Roman chicks at the age of 1-2 months and non-immune chick embryos at the age of 9-11 days; the 10-11-day-old susceptible duck embryos are prepared; according to a 9-11-day-old SPF (specific pathogen free) chicken embryo, the materials are used for preparing antigens and concentrating the antigens, and a concentrated vaccine immunized chicken prepared by applying an ND, IB and AI virus antigen concentration technology is used, so that the HI antibody levels of ND (8.0 Log2) to 10.1 Log2, IB (6.5 Log2) to 8.5 Log2 and AI (7.5 Log2) to 8.9 Log2 can be obviously improved, the immune efficacy of the vaccine is improved, the HA titers of ND, IB and AI virus antigens are improved by 4 times after concentration, and the HA titers of the ND, IB and AI virus antigens are improved by 4 times after concentration. It is ensured that the concentration of antigens contained in the quadruple vaccine is equal to the concentration of antigens contained in ND, IB and AI single vaccines.

The application belongs to the technical field of food processing equipment, and particularly relates to a low-temperature circulating pickling equipment for preparing roasted duck, which comprises a rack, a roller installed on the rack, a vacuum mechanism and a cooling mechanism installed in the rack, an outer shaft on which the roller is installed, an inner shaft concentrically installed in the outer shaft, a transmission mechanism for driving the outer shaft and the inner shaft to rotate reversely, stirring blades installed on the inner wall of the roller, an extrusion layer installed on the surface of the inner shaft, a cavity formed in the extrusion layer, and a gap for passing duck embryos between the extrusion layer and the stirring blades. The low-temperature circulating pickling equipment for preparing roasted duck has a simple structure, and the duck embryos can be extruded, kneaded and turned over by the extrusion layer expanded under vacuum and the stirring blades, so that the contact between the duck embryos and the pickling materials is improved, the absorption of the pickling materials by the duck embryos is promoted, the pickling effect of the duck embryos is improved, and the stirring blades in the roller are not fixed, so that the cleaning effect in the roller is improved.

The application discloses a long-chain non-coding RNA molecule lnc46198 related to development of sex glands of semi-muscovy ducks in an embryonic period and application thereof. The sequence of lnc46198 is shown as SEQ ID NO. 1. It is found by the application that lnc46198 is specifically expressed in sex glands of semi-muscovy ducks in an embryonic period, and inhibits proliferation of duck embryo fibroblasts and promotes apoptosis of duck embryo fibroblasts. It is shown that lnc46198 may affect development of sex glands of semi-muscovy ducks in an embryonic period by inhibiting cell proliferation activity, lays a foundation for functions of key lncRNAs in differentiation and development of germ cells of semi-muscovy ducks and the like in the process of differentiation and development of sex glands in an embryonic period, and provides a scientific reference basis for an occurrence mechanism of a sterile trait of semi-muscovy ducks.

The invention discloses a method for separating and amplifying duck circovirus by using duck embryo primary hepatocytes, and relates to the technical field of animal virology and cell culture. According to the method, a trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) and collagenase IV double-enzyme digestive system is creatively combined and the HGF is added, so that the yield and the activity of primary hepatocytes are remarkably improved, and the duck circovirus is quickly separated on the basis of the yield and the activity of the primary hepatocytes. According to the technical scheme, the duck embryo liver is adopted as a cell source, the cell survival rate is increased by 16.5% by shortening the digestion time and optimizing the enzyme ratio, an HGF stable cell membrane structure is innovatively introduced, a complete DuCV in-vitro proliferation system is established, and the virus titer reaches a peak value 60 hours after inoculation. According to the method, the duck circovirus is rapidly separated and amplified by using the duck embryo primary hepatocytes, and the living cell percentage of the primary cells is improved, so that the problems of low duck circovirus separation efficiency, low primary cell utilization rate, high cost and the like in the existing method are solved.

The utility model relates to the technical field of roast duck processing equipment, in particular to a hook for roast duck processing, which comprises a main hook and an auxiliary hook, the main hook is hooked between the neck root and the rib of a duck embryo and used for being hung on the roast equipment, and the auxiliary hook is arranged on the main hook and hooked on the inner side of the wing root of the duck embryo. One end of the main hook hooks the neck root and the middle of ribs of a duck embryo, the other end of the main hook is connected with roasting equipment, one end of the auxiliary hook is connected with the main hook, the other end of the auxiliary hook is nailed into the duck wing root position, and if the main hook is hooked into the duck neck position, the auxiliary hook still hooks the duck wing root when the main hook is separated from the duck embryo due to ripening and tearing in the roasting process, so that the roast duck is prevented from falling off; therefore, the problem that the neck is torn and unhooked due to product curing in the traditional hook baking process is solved, the unhooking phenomenon of roast ducks in the equipment in the baking process is reduced, and the roast duck finished product rate is improved.

This invention discloses a method for producing fermented duck with low nitrite residue, characterized by the following steps: marinating duck meat in 1100-1200 mL of marinade per kilogram of duck meat, ensuring the duck embryo is completely submerged in the liquid, for 20-28 hours at a temperature of 0-4°C; then ultrasonically cleaning the duck meat at 150-400W for 1-4 minutes, followed by air drying; and then uniformly spraying a fermentation agent composed of *Lactobacillus fermentum* and *Hansophilus d'Barry* onto the surface of the duck embryo at an inoculation amount of 10... 6 ~10 8 CFU / g, constant temperature and humidity chamber program temperature 14~18℃, relative humidity 65~70%, air drying for 6~8 days, to obtain fermented duck with low nitrite residue. The advantages are that it can better maintain the color of duck meat, efficiently degrade nitrite, inhibit fat oxidation and rancidity, delay the increase of TVB-N value, and extend the product shelf life.

The invention relates to the technical field of food processing, and discloses a roast duck production process which comprises the following steps: firstly, performing inflation, skin scalding, sugar coating and low-temperature large-air-volume drying on a duck embryo to reduce the moisture of a skin layer to a set range; secondly, pouring a sol-state gel soup base into the abdominal cavity, cooling and gelatinizing the sol-state gel soup base, and meanwhile, spraying a weakly-alkaline oil-water micro-emulsified penetrating fluid onto the skin; then carrying out instantaneous high-temperature impact at 228-232 DEG C for about 8 minutes, so that a porous bulking layer is formed on the epidermis; and finally, quickly passing through the maximum ice crystal generation zone through the ultralow-temperature quick-freezing tunnel. According to the method disclosed by the invention, by utilizing a gel phase change support and enzymolysis tenderization technology and cooperating with efficient heat transfer and interface catalytic action of a micro-emulsification medium, epidermis coloring and texture recombination within a short time are realized. According to the process, the baking time is shortened, the water loss of meat is reduced, the problems that the skin of the quick-frozen roast duck is softened and the meat is dry and hard after the quick-frozen roast duck is reheated are effectively solved, and the production efficiency and sensory quality of the product are improved.

The utility model discloses a duck airing cabinet, which belongs to the technical field of food processing instruments and comprises a bottom box and a cabinet body, the cabinet body is arranged above the bottom box and connected with the bottom box through a plurality of screws, two display racks are further arranged inside the cabinet body, and a first light-emitting plate and a second light-emitting plate which are sequentially arranged from top to bottom are mounted in the display racks. The bottom end of the first light-emitting plate and the bottom end of the second light-emitting plate are light-emitting faces, a plurality of LED lamps and two duck hanging rods are arranged below the first light-emitting plate and the second light-emitting plate, the LED lamps and the duck hanging rods are all fixed to the display rack, and a first hole plate is further installed on the bottom face in the cabinet body. By means of the duck airing cabinet, ducks can be aired, the air drying process of fresh duck embryos can be displayed to customers, and the practicability of the duck airing cabinet is improved.

The invention relates to the technical field of food processing, in particular to a roast duck process method which comprises the following steps: roasting raw duck embryos, cooling roast ducks discharged from a furnace, and putting the cooled roast ducks into a refrigeration workshop for cooling until duck meat is compact; the roast duck is sliced, duck meat is cut open in a scaly shape, and the root of the duck meat is kept to be connected to the duck body; and integrally loading the roast duck into a food-grade vacuum bag, vacuumizing, and then integrally loading the roast duck into a commodity bag for plastic packaging, so as to complete the production of the roast duck product. The method is used for overcoming the defects that the roast ducks are difficult to slice when entering families, the pre-sliced roast ducks are easy to oxidize and adhere during storage and the taste is poor after secondary heating, the roast duck products are convenient to slice, good in preservation property and high in eating experience, and household consumption of the roast ducks is promoted.