40 results about "Goose parvovirus" patented technology

The invention provides a goose parvovirus strain with preservation number of CCTCC NO:V201811. The goose parvovirus strain can be used for preparing a vaccine for preventing goose parvovirus derived from muscovy duck. A prepared inactivated vaccine for the goose parvovirus is good in safety, and local or general adverse reactions caused by the vaccine are avoided. Analysis of characters, safety testing and potency testing data in storage life tests proves that various indexes are stable and effective; the immune effect of the vaccine is evaluated with a serological method and an immune challenge method, and results show that the prepared inactivated vaccine can provide effective immune protection for muscovy ducks and has good commercial development prospect.

The invention provides application of a perylenequinone compound in the preparation of feed for preventing and treating poultry viruses, and belongs to the technical field of biological engineering and agriculture. The invention relates to the preparation of the feed for preventing and treating the poultry viruses such as Marek's disease virus, avian leukosis virus subgroup J, chicken infectious anemia virus, avian reovirus, newcastle disease virus, infectious bursal disease virus, goose parvovirus, duck plague virus, avian influenza virus and the like by adding the perylenequinone compound into poultry feed. The perylenequinone compound comprises hypocrellin, elsinochrome, polyanticin, cladosporin, calphostin, cercosporin and the like, and has a series advantages of being convenient to extract, having good stability, having no obvious side effect for oral administration, realizing quick metabolism and the like. The dosage of the perylenequinone compound in the preparation of the feedfor preventing and treating the poultry viruses is 1-50umol of perylenequinone compound per kg by weight every day; the dosage of the perylenequinone compound in the preparation of the feed for preventing and treating the poultry viruses is 50umol-200umol of perylenequinone compound per kg by weight; moreover, the perylenequinone compound has very obvious effect of preventing and treating the poultry viruses, meanwhile can be produced through modern biotechnology, and can bring good economic and social benefits.

The invention discloses a recombinant Newcastle disease virus expressing goose parvovirus VP3 gene and a construction method thereof, belonging to the field of recombinant virus vaccines. The recombinant Newcastle disease virus is an isolated strain of goose origin, the cleavage site amino acid of its F protein is GRQGRL, and the P3 gene is located in the non-coding region between the P gene and the M gene of the Newcastle disease virus. The transcription plasmid pCI-NA-VP3 (SEQ ID NO.1) and the transcription helper plasmids pcDNA-N, pcDNA-P, pcDNA-L (SEQ ID NO.2-4) are co-transfected into host cells with Newcastle disease virus replication permission , culturing the transfected host cells, and the recombinant Newcastle disease virus can be rescued from the cell suspension of the transfected host cells. The recombinant Newcastle disease virus expressing the goose parvovirus VP3 gene of the invention can be used as a dual live carrier vaccine for preventing Newcastle disease and goose parvovirus.

The invention discloses a method for rapidly constructing a differentially expressed gene library, comprising the following steps: first extracting total mRNA of goose bursa infected with goose parvovirus and non-infected goose parvovirus; using the obtained mRNA to synthesize double-stranded cDNA for enzyme digestion Digestion, and then divided into 2 tubes, adding different adapters for connection; first perform forward subtractive hybridization on the double-stranded cDNA with adapters, and then perform reverse subtractive hybridization on the forward subtractive hybridization products, and then reverse subtractive hybridization Perform the first PCR amplification, dilute the amplified product, and use the diluted solution as a template to perform the second PCR amplification; finally, the product of the second PCR amplification is subjected to denaturing gradient gel electrophoresis, and stained after electrophoresis. The differentially expressed gene library of goose parvovirus stressing the bursa of Fabricius is obtained; the gene library constructed by the method of the invention has no false positive, and has the characteristics of fast speed, high sensitivity, strong specificity, low cost, convenient operation and the like.