Goose parvovirus protein capable of inducing goose embryo fibroblast apoptosis and preparation method thereof

A technology for fibroblasts and goose parvovirus, which is applied in the field of goose parvovirus protein and its preparation, can solve problems such as no literature clarification, no GPV-induced cell apoptosis, etc.

Inactive Publication Date: 2020-09-25
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on GPV is focused on the diagnosis and prevention of diseases. The pathogenic mechanism of GPV has not been clarified in the literature, and there is no relevant report on GPV-induced apoptosis.

Method used

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  • Goose parvovirus protein capable of inducing goose embryo fibroblast apoptosis and preparation method thereof
  • Goose parvovirus protein capable of inducing goose embryo fibroblast apoptosis and preparation method thereof
  • Goose parvovirus protein capable of inducing goose embryo fibroblast apoptosis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A goose parvovirus protein capable of inducing apoptosis of goose embryo fibroblasts is characterized in that the protein is a non-structural protein, and the amino acid sequence of the protein is shown in SEQ ID NO.1.

[0057] A goose parvovirus protein capable of inducing apoptosis of goose embryo fibroblasts can be prepared through the following steps:

[0058] (1) PCR method to amplify the target gene fragment:

[0059] (1.1) NS1 primer design

[0060] Forward and reverse primers with cleavage sites BamHI, XhoI and protective bases were designed based on the NS1 gene of GPV, and the sequences are shown in the table below;

[0061] Table 1 Primer Sequence

[0062]

[0063] (1.2) PCR amplification

[0064] Extract the genome of GPV according to E.Z.N.A.@Viral DNA Kit (OMEGA), and use the genomic DNA as a template to amplify the NS1 fragment. After the reaction, take the PCR product for 1% agarose gel electrophoresis, and it can be seen clearly at 1884bp The tar...

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PUM

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Abstract

The invention provides a goose parvovirus protein capable of inducing goose embryo fibroblast apoptosis. The goose parvovirus protein is a non-structural protein, the amino acid sequence of the protein is shown as SEQ ID NO.1, and the protein has a function of inducing goose embryo fibroblast apoptosis. The goose parvovirus protein has the beneficial effects that: the goose parvovirus protein capable of inducing goose embryo fibroblast apoptosis provided by the invention provides a theoretical basis for exploring a pathogenic mechanism of GPV infection on GEF cells, also provides a new thoughtfor preventing and treating GPV, and provides a direction for deep discussion of development of antiviral drugs and vaccines.

Description

technical field [0001] The invention belongs to the technical field of preventive veterinary medicine, relates to a protein capable of inducing apoptosis of goose embryo fibroblasts, in particular to a goose parvovirus protein capable of inducing apoptosis of goose embryo fibroblasts and a preparation method thereof. Background technique [0002] Goose Parvovirus (GPV) can cause a serious disease called Derzsy disease, which affects goslings and Muscovy ducks. Mortality may reach 100% in susceptible birds less than 1 week old. High mortality rates (10-60%) also occur in susceptible birds of 1-3 weeks of age. [0003] Virus replication not only requires the continuous multiplication and transmission of progeny, but also limits the replication of host cells by killing recipient cells. In addition to inducing immune and inflammatory responses, infection with most viruses also triggers apoptosis or other forms of death of infected cells, a cellular response that is often a man...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/015C12N15/85C12N15/66
CPCC07K14/005C12N15/66C12N15/85C12N2750/14322C12N2750/14351C12N2800/106
Inventor 董浩胡桂学王开鄢雨晴张双刘军亭
Owner JILIN AGRICULTURAL UNIV
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