3 results about "Peptide display" patented technology

This invention provides a method for screening peptides that specifically target T cells, comprising providing T cells having a subset selected from CD1, CD2, CD3, CD4, CD5, CD7, CD8, CD16, CD25, CD26, CD27, CD28, CD30, CD38, CD39, CD40L, CD44, CD45, CD62L, CD69, CD73, CD80, CD83, CD86, CD95, CD103, CD119, CD126, CD150, CD152 (CTLA-4), CD153, CD154 (CD40L) The T cells are labeled with at least one of the following surface antigen markers: CD161, CD183, CD223, CD254, CD275, CD45RA, CXCR3, CXCR5, FasL, IL18R1, CTLA-4, OX40, GITR, LAG3, ICOS, PD-1, leu-12, TCR, TLR1, TLR2, TLR3, TLR4, TLR6, NKG2D, CCR, CCR1, CCR2, CCR4, CCR6, and CCR7. The T cells are then contacted with a peptide display library, and target peptides that specifically bind to the T cells are screened therefrom.

The application provides a mycobacterium tuberculosis small peptide display library and a construction method and application thereof, and the construction method comprises the following steps: S1, small peptide sequence information is obtained by intercepting according to mycobacterium tuberculosis proteome sequence information; S2, the amino acid sequence of the small peptide sequence information is converted into a codon sequence, sequence optimization is carried out, and after a consensus sequence is added, oligonucleotide sequence information is obtained; S3, an oligonucleotide physical object is synthesized in a large scale and in parallel by using a chemical method; S4, PCR amplification is carried out by taking the oligonucleotide physical object as a template, and after being digested by a restriction endonuclease, an inserted nucleic acid fragment is obtained; and S5, the inserted nucleic acid fragment is used to construct the small peptide display library by using a display system. The mycobacterium tuberculosis small peptide display library constructed by the application provides important tools and resources for systematically understanding mycobacterium tuberculosis immunological epitopes, vaccine development, new drug research and development and the like.

To provide a novel cyclization method for peptide compounds to construct a peptide display library, and to provide methods for the synthesis of branched peptides.SOLUTION: Provided is a method for producing a peptide compound having a cyclic portion, the method comprising: 1) translating and synthesizing an acyclic peptide compound from a nucleic acid encoding the peptide compound composed of an amino acid residue and / or amino acid analog residue, or an amino acid residue and / or amino acid analog residue and N-terminal carboxylic acid analog, the acyclic peptide compound containing an amino acid residue or amino acid analog residue having a reactive site in one of its side chains, and an amino acid, amino acid analog residue or N-terminal carboxylic acid analog with another reactive site at the N-terminus; and 2) forming a carbon-carbon bond between the reactive site of the N-terminal amino acid residue, N-terminal amino acid analog residue, or N-terminal carboxylic acid analog and the reactive site of the amino acid residue or amino acid analog having a reactive site in one of its side chains.SELECTED DRAWING: None