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Polypeptide specifically bound to surface of SGC-7901 cell

A technology of SGC-7901, 1. SGC-7901 is applied in the field of peptides specifically bound to the surface of SGC-7901 cells, which can solve the problems of high degree of malignancy, low early diagnosis rate, and impact on the prognosis of gastric cancer.

Inactive Publication Date: 2013-09-11
SHAANXI NORMAL UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0002] Gastric cancer is one of the common malignant tumors of the digestive tract. It has high malignancy, low early diagnosis rate, and poor prognosis. Postoperative recurrence and metastasis are the main reasons affecting the prognosis of gastric cancer.

Method used

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  • Polypeptide specifically bound to surface of SGC-7901 cell
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  • Polypeptide specifically bound to surface of SGC-7901 cell

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Embodiment Construction

[0026] 1. Technical route

[0027] The present invention adopts phage polypeptide display technology, takes the human gastric cancer SGC-7901 cell line as the target cell, and uses the human embryonic kidney HEK293 cell as the negative adsorption cell to carry out four rounds of subtractive screening, and screens from the phage 12 peptide library that can specifically bind to gastric cancer SGC- The peptide motifs of 7901 cells are searched for cell surface receptors that may be recognized, which will provide a good experimental basis for further research on early diagnosis and targeted therapy of gastric cancer. The specific technical routes are as follows: figure 1 shown.

[0028] 2. Materials and methods

[0029] 2.1 Experimental materials

[0030] 2.1.1 Cell lines, phage display random peptide library, host bacteria

[0031] (1) Cell lines: human embryonic kidney cell line HEK293, human gastric cancer cell line SGC-7901, provided by our laboratory.

[0032] (2) Phage d...

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Abstract

The invention discloses polypeptide specifically bound to surface of an SGC-7901 cell. A random dodecapeptide library is displayed by bacteriophage to obtain five polypeptide segments in a screening manner; the amino acid sequences respectively are YTHNESSPKDTH, YTVPDYKHNSAH, THPWQITSVNFK, DVFPHSRPADEL and IHKDPANKSLVP. A polypeptide sequence bound to a gastric cancer SGC-7901 cell is screened by utilizing a phage peptide display technology; the affinity of phage clones and the gastric cancer is identified by enzyme-linked immuno sorbent assay (ELISA); 14 phage clones are obtained; five polypeptide sequences are obtained by sequencing. The homology analysis shows that the polypeptide sequence motif can be amino acid determinants bound to a tumor cell surface receptor on ligandin; the targeted results of positive phage clones are further identified by cell immunofluorescence to prompt the positive phage clones to be specifically bound to the SGC-7901 cell; an experimental evidence is provided for early diagnosis of the gastric cancer, targeted transportation of an antitumor medicament, and research and development of targeted short-peptide medicaments by the screened gastric cancer SGC-7901 cell specificity polypeptide.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to screening tumor tissue-specific binding peptides, in particular to a polypeptide specifically binding to the surface of SGC-7901 cells. Background technique [0002] Gastric cancer (gastric cancer) is one of the common malignant tumors of the digestive tract. It has high malignancy, low early diagnosis rate, and poor prognosis. Postoperative recurrence and metastasis are the main reasons affecting the prognosis of gastric cancer. Therefore, exploring the cellular and molecular biological mechanisms of the occurrence and development of gastric cancer is crucial to the current treatment of gastric cancer. At the same time, finding early diagnosis methods with high sensitivity and specificity is also a preferred strategy to reduce the mortality of gastric cancer. In recent years, the rapid development of molecular imaging and targeted therapy has found new hope for the treatment of gastri...

Claims

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Application Information

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IPC IPC(8): C07K7/08
Inventor 侯颖春马彩霞何慧敏高宁
Owner SHAANXI NORMAL UNIV
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