4 results about "Coat Proteins" patented technology

The application discloses a kind of MS2 virus-like particles based on capsid protein site-directed mutation and its preparation method and application, belong to molecular biological technique field.The amino acid of the beta sheet interface of capsid protein sequence, the amino acid of the interface when two capsid proteins molecules assemble, the carboxyl end site of capsid protein is subjected to site-directed mutation, obtain the mutant that can significantly improve the assembly efficiency and stability of capsid protein dimer.Utilize the mutant to construct MS2 virus-like particles, further by the design of mature enzyme and capsid protein expression element, control mature enzyme weak expression, capsid protein strong expression, to improve assembly efficiency.The application improves the assembly efficiency of MS2 virus by the above strategy, improves the proportion of wrapped RNA, enhances the stability of virus-like particle, greatly prolongs the stable storage time of RNA, improves the purity of wrapped RNA.

The application discloses a New Delhi tomato leaf curl virus coat protein polypeptide, antigen, antibody and application thereof, and belongs to the technical field of organism immunity detection. The application is based on ToLCNDV CP to design a polypeptide sequence, and provides a New Delhi tomato leaf curl virus coat protein polypeptide. The antigen prepared by coupling the polypeptide with keyhole limpet hemocyanin (KLH) can obtain a New Delhi tomato leaf curl virus antibody with high sensitivity and specificity, and can be used for screening the virus, and realizes instant detection and early warning of the ToLCNDV virus. Meanwhile, the application provides a theoretical basis and technical support for establishment of a virus serological diagnosis method and related detection product research and development, and is helpful to development of more accurate, efficient and portable detection products, so as to better serve the virus monitoring, quarantine and prevention and control work.

The present invention relates to a modified virus-like particle (VLP) of Cucumber Mosaic Virus (CMV) comprising at least one fusion protein, wherein the at least one fusion protein comprises or preferably consists of b) a chimeric CMV polypeptide, wherein the chimeric CMV polypeptide comprises or preferably consists of (iii) a CMV polypeptide, wherein the CMV polypeptide comprises a coat protein of CMV; and (iv) an antigen polypeptide, wherein the antigen polypeptide is inserted into the CMV polypeptide, wherein the insertion of the antigen polypeptide is between amino acid residues corresponding to position 84 and position 85 of SEQ ID NO: 62 of the CMV polypeptide; and (iii) a T helper cell epitope, wherein the T helper cell epitope replaces an N-terminal region of the CMV polypeptide, and wherein preferably the N-terminal region of the CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO: 62.