76 results about "Coat Proteins" patented technology

Aspects of the invention provide modified virus-like particles that are designed for therapeutic applications. In particular, aspects of the invention provide CCMV coat proteins that are modified to generate virus-like particles, including mosaic virus-like particles, that can package and/or deliver one or more diagnostic and/or therapeutic agents. The invention also provides methods for treating subjects with one or more modified virus-like particles.

The invention relates to a polypeptide, in particular to a human papilloma virus coat protein L1 short peptide and relative application. The invention is characterized in that the sequence of the human papilloma virus coat protein L1 short peptide is N-EVNLKEKFSADLDQFPLGRKFLLQAGLKAK-C. The invention can simultaneously induce human papilloma virus coat protein L1 short peptide with immunity reaction on high risk type and low risk type, which can induce and form the antibody for various human papilloma virus (HPV) and coat protein (HPV L1), to check various HPV or HPV L1, while the antibody can be used in biological pharmaceutical engineering, to purify and prepare various HPV or HPV L1.

The invention provides a herpes virus EBV (Epstein-Barr Virus) detection kit. The kit comprises a nucleic acid releasing agent and PCR (polymerase chain reaction) reaction solution, wherein the nucleic acid releasing agent comprises 0.01-0.5 mM/L of surfactin, 20-300 mM/L of potassium chloride, 0.01-2% of sodium dodecyl sulphate and 0.05-1% of ethanol; and the PCR reaction solution comprises an upstream primer and a downstream primer used for target polynucleotide amplification, and a probe used for target polynucleotide detection. The detection result of the method for releasing nucleic acid by the nucleic acid releasing agent in the kit disclosed by the invention has no obvious difference with the detection result of a boiling method, a strong protein denaturing agent is used during nucleic acid extraction in the kit disclosed by the invention for rapidly breaking the coat protein structure of a pathogen and releasing the nucleic acid of the pathogen, and release and extraction for DNA (deoxyribonucleic acid) can be rapidly finished without heating; the sensitivity of the EBV detection of the kit disclosed by the invention can achieve 400 copies/ml, and the quantitative linear range is 400-4.00E+09 copies/ml; by applying the kit, rapid and accurate detection can be performed on EBV-DNA in the unknown samples of blood plasma, throat swab, peripheral blood and the like, and reliable experimental basis is provided for diagnosing EBV infection.

The invention provides a bran coat antitumor active protein, a preparation method of the protein, and applications of the protein in preparing antitumor drugs and health foods. The preparation method of the protein comprises the steps of pulverizing all natural bran coat, adding protein extracting solution, extracting at low temperature after one night, then adding precooled acetone to precipitate protein at low temperature so as to obtain bran coat protein, adding ammonium sulfate powders with different saturation levels into coarse protein extracting solution for fractional precipitation of the protein, enabling the protein precipitated by the ammonium sulfate to permeate through a Q anion exchange chromatographic column, collecting penetrating fluid, enabling the penetrating fluid to permeate through the Q anion exchange chromatographic column again, performing elution with Tris-NaCl eluant with the concentration of 0.1mol/L, and collecting eluting peaks to obtain the bran coat antitumor active protein (FMB). The protein can obviously restrain the proliferation and the metastasis of colorectal cancer cells and can be applied to preparation of the antitumor drugs and the health foods.

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.

The invention discloses preparation, detection and application of a polyclonal antibody of a Yam mild mosaic virus (YMMV). Based on a YMMV genome, the inventor purifies a nuclear inclusion protein/coat protein (NIb/CP) of the YMMV through prokaryotic expression and uses the nuclear inclusion protein/coat protein to prepare the polyclonal antibody for an immune rabbit. The polyclonal antibody can specifically identify the Nib/CP protein of YMMV through prokaryotic expression and the Nib/CP protein of the YMMV virus which infects common yam rhizome, and generates specific immunity response. On the basis, the inventor further builds a set of efficient, high sensitive and accurate IC-RT-PCR (Immunocapture-Reverse Transcription-Polymerase Chain Reaction, ELISA (Enzyme-Linked Immuno Sorbent Assay) and Western blot methods which can specifically detect the YMMV from a common yam rhizome plant which is infected by YMMV. By applying the invention, a material basis and a technical support can be provided for research on interaction of the YMMV and the host, quick detection of the virus and somatotype and molecular biology study, thereby laying a solid foundation for epidemiologically monitoring and preventing treating the virus.

The invention discloses a hybridoma cell strain capable of secreting a tomato yellow leaf curl virus (TYLCV) monoclonal antibody and application of the monoclonal antibody. A coat protein gene of a tomato yellow leaf curl virus separator (TYLCV-SH2) is cloned; the coat protein of the virus is expressed by a prokaryotic expression system; BALB/c mice are immunized by using expression and purification protein as antigen; a hybridoma cell strain D10 capable of performing passage stably and secreting the TYLCV monoclonal antibody is obtained through cell fusion, screening and cloning; and the collection number is CGMCC No.5538. The D10 monoclonal antibody ascites indirect ELISA valence reaches more than 10<-6>; and the antibody type and subclass are IgG1 and kappa chains. A dot-ELISA detection method for detecting the TYLCV of the tomatoes is established by the D10 monoclonal antibody; and when the leaf suffering from the disease is diluted according to the ratio of 1:320 (w/v and g/mL), the virus can still be detected. By the dot-ELISA and Tissue-blot ELISA method, the TYLCV of tomato samples in the field can be detected accurately, specifically and sensitively. Due to establishment of a preparation method for the TYLCV monoclonal antibody and a detection method, technological and material support is provided for diagnosis, prediction and scientific prevention and control of the tomato virus disease.

The invention provides for a composition comprising a genetically engineered bacteriophage capable of guiding cell growth and polarization via signaling peptides and directionally aligned structures. The invention provides for modified bacteriophage and its uses thereof. The present invention also provides for genetically engineered phage capable of guiding cell growth, migration and/or alignment, providing essential biological effects including proliferation and/or differentiation, which can be performed by expressing specific biological motifs, such as the amino acid sequences RGD, IKVAV, DGEA and HPQ, on their coat proteins, on which functional DNA, proteins and cells can be conjugated and/or fixed thereon.

The present invention provides a method of enhancing resistance of plants to one or multiple viruses, comprising introducing to a plant cell, and preferably expressing therein, a nucleotide sequence encoding a virus-encoded polypeptide. The present invention provides a method of enhancing the proportion of virus-resistant or virus-immune lines obtained from a single transformation experiment comprising introducing to a plant cell, a nucleotide sequence encoding a virus-encoded polypeptide operably in connection with a strong promoter sequence. The present invention provides novel gene sequences encoding the coat proteins of a virus and novel dysfunctional replicase sequences as well as gene constructs comprising same, in particular binary vector constructs suitable for introducing into plants and expressing the genes therein. The present invention provides and methods using same to enhance viral resistance in plants. The present invention provides novel methods of testing transgenic plants for the presence of a transgene.

The invention relates to the technical field of biology, in particular to a COVID-19 pseudovirus as well as a preparation method and application thereof. The COVID-19 pseudovirus is formed by virus packaging of coat protein particles and helper plasmids, wherein the coat protein particles comprise plasmids for expressing a COVID-19 S protein, plasmids for expressing a COVID-19 M protein and plasmids for expressing a COVID-19 E protein. The COVID-19 pseudovirus is packaged by adopting a three-plasmid system, and the S/M/E protein is used for replacing expression a VSV-G protein, so that the COVID-19 pseudovirus is stronger in infection capability and higher in sensitivity compared with the pseudovirus only containing the S protein. Moreover, the COVID-19 pseudovirus carries two fluorescentreporter groups, and different fluorescent reporter groups can be applied to different scenes, so that the COVID-19 pseudovirus is simpler and more convenient to apply.

The invention relates to a detection method for sugarcane mosaic virus in corn. According to the detection method, a high-quality virus RNA sample is extracted, primers are designed according to the coat protein (CP) genes of the sugarcane mosaic virus, then detection is carried out by utilizing RT-PCR and Real-time RCR technologies, and finally a set of rapid and efficient virus detection method is established. According to the detection method, by effectively detecting the sugarcane mosaic virus with low content in the corn, the potential hazard of the sugarcane mosaic virus is reduced, and the loss of the crop production is reduced.

The invention belongs to the technical field of plant genetic engineering, and discloses a potyvirus virus-induced gene silencing (VIGS) system with a papaya leaf distortion mosaic virus (PLDMV) as avirus vector. According to the system, a target gene is inserted between Nib and coat protein (CP) of the PLDMV, and a NIa-Pro digestion site is introduced between the target gene and the CP. By in-vitro splicing methods such as Gibson splicing or In-Fusion cloning, target gene fragments are seamlessly cloned and inserted between the Nib and the CP to construct a recombinant virus silence vector containing a plant target gene. Plants are inoculated with the recombinant virus silence vector by agrobacterium injection, and phenotypic changes of the plants are observed after the target gene silencing the plants is induced. The potyvirus VIGS system provides a powerful tool to study gene functions of papaya by using the PLDMV silence vector, and has great scientific significance and application prospects.

The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.

The invention discloses a colloidal gold immunization test strip and a detection method for detecting plum pox viruses. The colloidal gold immunization test strip is formed by the steps of: A. extracting RNA (Ribonucleic Acid) of plum pox viruses from materials infected by the plum pox viruses; B. cloning coat protein genes of the plum pox viruses by an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method, connecting the coat protein genes on a prokaryotic expression vector pET29(a) to obtain pET29a-PPV (porcine parvovirus) recombinant vectors, orderly measuring and finally verifying the recombinant vectors with correct reading frames, carrying out the inducible expression with the coat protein genes of the plum pox viruses, and transforming escherichia coli BL21 (DE3) with the pET29a-PPV recombinant vectors; C. recovering specific expression protein by an affinity column method, and making the rabbits immune to obtain plum pox virus antiserum; and D. adopting the above antiserum to prepare the colloidal gold immunization test strip used for detecting the plum pox viruses by the prepared antiserum. The invention aims to provide the convenient colloidal gold immunization test strip for rapidly detecting the plum pox viruses and the method for detecting plum pox viruses by adopting the test strip.

The invention discloses a recombinant human VEGF tumor polypeptide vaccine. Human VEGF epitope nucleotide sequence is acquired by a synthesizing process and is inserted to the terminal C of coat protein gene of bacteriophage QBeta. Through a coexpression system, coat protein gene of bacteriophage QBeta and the coat protein gene of bacteriophage QBeta of which the terminal C is inserted with the VEGF epitope are expressed simultaneously in escherichia coli BL21(DE3), and QBeta virus-like particles with human VEGF epitope presented on surfaces are obtained after the expression product is purified. The recombinant human VEGF-QBeta protein produces human VEGF antibody through immunized mice; the human VEGF antibody is capable of neutralizing biological activity of human VEGF 165 when applied to the HUVEC cell proliferation test. When applied to immunization of mice, the homologous mouse VEGF polypeptide vaccine which is produced by the presenting way has a certain inhibition effect to TC-1 tumor cells in bodies of C57 BLACK6 mice.

The invention provides application of reducing expression of OsSAMS1 protein and an encoding gene thereof in improving plant resistance to rice dwarf virus. The amino acid sequence of the protein OsSAMS1 is a sequence 2 in a sequence table. The experiments prove that RDV coat protein Pns11 can interact with the rice OsSAMS1 protein, so that the OsSAMS1 enzyme activity is increased, the OsSAMS1 protein excessively-accumulated rice is more susceptible to diseases, and the transgenic rice with the OsSAMS1 eliminated by a CRISPR/Cas9 pathway has enhanced resistance to the RDV.

The invention provides a process method for coating protein with magnetic beads, and belongs to the technical field of processes for coating protein with magnetic beads. The method comprises the following steps of: firstly, carrying out antigen/antibody replacement to obtain a replaced to-be-coated object; cleaning the magnetic beads; taking a cleaned resuspended magnetic bead bottle, placing theresuspended magnetic bead bottle on a 360-degree uniform mixing instrument, and premixing the magnetic beads with the replaced to-be-coated object in a horizontal swing uniform mixing manner, the concentration of the magnetic beads being 5mg/mL; adding eDC and NHS into the premixed magnetic beads, putting the magnetic beads on the 360-degree uniform mixing instrument for activation, wherein the mass ratio of EDC to the magnetic beads is 1:10; and adding a confining liquid, collecting magnetism to remove supernatant, and performing cleaning to obtain magnetic beads coated protein. According tothe process method, the protein coating efficiency can be improved, and the test performance of the corresponding kit is improved.