13 results about "Coat Proteins" patented technology

The invention discloses a composite material, a preparation method of the composite material, a thin film and a photoelectric device, the composite material comprises tobacco mosaic virus and metal nanoparticles, and the metal nanoparticles are combined with coat protein of the tobacco mosaic virus. Part of peptide chains in the coat protein of the tobacco mosaic virus and different types of metal nanoparticles have special binding energy, so that the metal nanoparticles can be orderly assembled on the coat protein of the tobacco mosaic virus, the problem that the metal nanoparticles are easy to agglomerate is effectively improved, and the composite material can be applied to photoelectric devices and has good application prospects. The photoelectric performance and the service life of the photoelectric device can be improved.

The application discloses a kind of MS2 virus-like particles based on capsid protein site-directed mutation and its preparation method and application, belong to molecular biological technique field.The amino acid of the beta sheet interface of capsid protein sequence, the amino acid of the interface when two capsid proteins molecules assemble, the carboxyl end site of capsid protein is subjected to site-directed mutation, obtain the mutant that can significantly improve the assembly efficiency and stability of capsid protein dimer.Utilize the mutant to construct MS2 virus-like particles, further by the design of mature enzyme and capsid protein expression element, control mature enzyme weak expression, capsid protein strong expression, to improve assembly efficiency.The application improves the assembly efficiency of MS2 virus by the above strategy, improves the proportion of wrapped RNA, enhances the stability of virus-like particle, greatly prolongs the stable storage time of RNA, improves the purity of wrapped RNA.

The invention discloses a protein nano material as well as a preparation method and application thereof. The protein nano material comprises a disc-shaped self-assembly body formed by tobacco mosaic virus capsid protein and a peptide fragment containing RGD motif and presented on the surface of the disc-shaped self-assembly body, the peptide fragment containing the RGD motif is presented on the surface of the discoid self-assembly body in a multivalent form, and the peptide fragment containing the RGD motif contains at least two cysteine residues. According to the protein nanomaterial, the tobacco mosaic virus capsid protein disc-shaped self-assembly body is used as a support, stable multivalent presentation of the RGD motif peptide fragment is achieved on the surface of the support, and therefore stable and consistent adhesion sites can be provided on the culture surface; meanwhile, the material composition is clear, the preparation process is controllable, the batch difference is reduced, and the repeatability and reliability of cell culture and related application are improved.

The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of capable of systemic and phloem-limited movement and replication within the host plant.

The application discloses a New Delhi tomato leaf curl virus coat protein polypeptide, antigen, antibody and application thereof, and belongs to the technical field of organism immunity detection. The application is based on ToLCNDV CP to design a polypeptide sequence, and provides a New Delhi tomato leaf curl virus coat protein polypeptide. The antigen prepared by coupling the polypeptide with keyhole limpet hemocyanin (KLH) can obtain a New Delhi tomato leaf curl virus antibody with high sensitivity and specificity, and can be used for screening the virus, and realizes instant detection and early warning of the ToLCNDV virus. Meanwhile, the application provides a theoretical basis and technical support for establishment of a virus serological diagnosis method and related detection product research and development, and is helpful to development of more accurate, efficient and portable detection products, so as to better serve the virus monitoring, quarantine and prevention and control work.

The invention provides a nano-microsphere immunochromatography test strip for specifically detecting potato virus M. The test strip comprises a PVC (polyvinyl chloride) bottom plate, and a sample pad, a conjugate pad, an NC (nitrocellulose) membrane and a water absorption pad which are sequentially overlapped on the PVC bottom plate along a sample chromatography direction, a polystyrene nano-microsphere labeled antibody is fixed on the combination pad, a detection line T line and a quality control line C line are arranged on the NC membrane, a coated antibody is marked on the T line, and a goat anti-chicken antibody is marked on the C line; the labeled antibody and the coating antibody are paired monoclonal antibodies specifically combined with potato M virus coat protein PVM-CP; the labeled antibody is PVM-1G5B2F2 and is an IgG type antibody, the amino acid sequence of a heavy chain variable region is as shown in SEQ ID No.1, a light chain is a K type light chain, and the amino acid sequence of a light chain variable region is as shown in SEQ ID No.2. The test strip disclosed by the invention has the advantages of high sensitivity, high specificity, good stability, simplicity and rapidness in operation, high detection accuracy, wide application range and the like, and shows a good application prospect in rapid field detection of the potato virus M.

The present invention relates to a modified virus-like particle (VLP) of Cucumber Mosaic Virus (CMV) comprising at least one fusion protein, wherein the at least one fusion protein comprises or preferably consists of b) a chimeric CMV polypeptide, wherein the chimeric CMV polypeptide comprises or preferably consists of (iii) a CMV polypeptide, wherein the CMV polypeptide comprises a coat protein of CMV; and (iv) an antigen polypeptide, wherein the antigen polypeptide is inserted into the CMV polypeptide, wherein the insertion of the antigen polypeptide is between amino acid residues corresponding to position 84 and position 85 of SEQ ID NO: 62 of the CMV polypeptide; and (iii) a T helper cell epitope, wherein the T helper cell epitope replaces an N-terminal region of the CMV polypeptide, and wherein preferably the N-terminal region of the CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO: 62.

The invention discloses a New Delhi tomato leaf curl virus coat protein polypeptide, an antigen, an antibody and application thereof, belongs to the technical field of organism immunodetection, and provides the New Delhi tomato leaf curl virus coat protein polypeptide based on design of a polypeptide sequence of ToLCNDV CP. The New Delhi tomato leaf curl virus antibody with relatively high sensitivity and specificity can be obtained through an antigen immune experiment body prepared by coupling the new Delhi tomato leaf curl virus antibody with KLH, and can be used for screening the virus, so that instant detection and early warning of the ToLCNDV virus are realized. Meanwhile, a theoretical basis and a technical support are provided for establishment of a virus serological diagnosis method and research and development of related detection products, and development of more accurate, efficient and portable detection products is facilitated, so that monitoring, quarantine and prevention and control work of viruses can be better served.

The application discloses application of glycoside compounds in prevention and treatment of plant pathogenic viruses and belongs to the technical field of pesticide antiviral agents.Taking a tobacco mosaic virus coat protein as an example, small molecules including sugar rings, such as Swertiamarin I (A0) and ZINC85593196 (B0), are screened through virtual screening, molecular dynamics simulation, molecular mechanics and MM / GBSA free energy calculation. Based on this, further optimization is carried out, and six compounds similar to the structure of A0, such as 4-methylumbelliferone-beta-D-xyloside (A1), gardenoside (A2), 4-methylumbelliferone-beta-D-pyranoside (A3), 4-methylumbelliferone-beta-D-pyranoglucoside (A4), catalpol (A5) and esculin (hemihydrate) (A6), are obtained, and in-vivo and in-vitro biological activity evaluation is carried out. The active ingredients screened by the above method have good inhibitory effect on plant viruses.

The application provides an adeno-associated virus mutant and application thereof, and the coat protein of the adeno-associated virus mutant comprises an amino acid sequence shown in SEQ ID NO: 1-5, or an amino acid sequence with more than 98% identity with SEQ ID NO: 1-5 and having the same or similar biological function. The application constructs an adeno-associated virus library by splicing and recombining a coat protein coding gene of a wild type adeno-associated virus, and obtains the adeno-associated virus mutant by screening with neutralizing antibodies against AAV coat proteins, the adeno-associated virus mutant has strong infection ability on human liver cells and can avoid the neutralization of neutralizing antibodies, and thus the technical obstacles existing in the field of hemophilia treatment are solved in a targeted manner.

The present invention relates to a modified virus-like particle (VLP) of Cucumber Mosaic Virus (CMV) comprising at least one fusion protein, wherein the at least one fusion protein comprises or preferably consists of b) a chimeric CMV polypeptide, wherein the chimeric CMV polypeptide comprises or preferably consists of (iii) a CMV polypeptide, wherein the CMV polypeptide comprises or preferably consists of a coat protein of CMV, wherein preferably the coat protein of CMV comprises or preferably consists of SEQ ID NO: 62; or an amino acid sequence having a sequence identity of at least 75%, preferably at least 80%, more preferably at least 85%, further preferably at least 90%, yet further preferably at least 95%, still further preferably at least 98% and yet still further more preferably at least 99% to SEQ ID NO: 62; and (iv) an antigen polypeptide, wherein the antigen polypeptide is inserted into the CMV polypeptide, wherein the insertion of the antigen polypeptide is between amino acid residues corresponding to amino acid residue of position 84 and position 85 of SEQ ID NO: 62 of the CMV polypeptide; and (iii) a T-helper cell epitope, wherein the T-helper cell epitope replaces an N-terminal region of the CMV polypeptide, and wherein preferably the N-terminal region of the CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO: 62.

This invention discloses a hybridoma cell line secreting monoclonal antibody PVY-2, its monoclonal antibody, and its applications. The hybridoma cell line 4F1B2G11 secretes a monoclonal antibody against potato virus Y. The preservation number of the hybridoma cell line is CCTCC NO: C2022281. The monoclonal antibody PVY-2 has a specific immunoreactivity with the coat protein of potato virus Y. The nucleotide sequence of the heavy chain variable region of monoclonal antibody PVY-2 is shown in SEQ ID NO.4, and the nucleotide sequence of the light chain variable region is shown in SEQ ID NO.5. The sensitivity of the direct ELISA method for detecting potato virus Y-infected leaves reaches a 1:5120 dilution. The monoclonal antibody PVY-2 can also be used to prepare ELISA kits, colloidal gold kits, or nano-enzyme kits, enabling rapid, sensitive, economical, and accurate detection of potato virus Y disease.

The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of systemic and phloem-limited movement and replication within the host plant.