89 results about "Coat Proteins" patented technology

Aspects of the invention provide modified virus-like particles that are designed for therapeutic applications. In particular, aspects of the invention provide CCMV coat proteins that are modified to generate virus-like particles, including mosaic virus-like particles, that can package and / or deliver one or more diagnostic and / or therapeutic agents. The invention also provides methods for treating subjects with one or more modified virus-like particles.

The invention relates to chimeric molecules comprising a virus coat sequence and a receptor sequence that can interact with each other to form a complex that is capable of binding a co-receptor. Such chimeric molecules therefore exhibit functional properties characteristic of a receptor-coat protein complex and are useful as agents that inhibit virus infection of cells due to occupancy of co-receptor present on the cell, for example. In particular aspects, the chimeric polypeptide includes an immunodeficiency virus envelope polypeptide, such as that of HIV, SIV, FIV, FeLV, FPV and herpes virus. Receptor sequences suitable for use in a chimeric polypeptide include, for example, CCR5 and CXCR4 sequences.

The invention relates to a polypeptide, in particular to a human papilloma virus coat protein L1 short peptide and relative application. The invention is characterized in that the sequence of the human papilloma virus coat protein L1 short peptide is N-EVNLKEKFSADLDQFPLGRKFLLQAGLKAK-C. The invention can simultaneously induce human papilloma virus coat protein L1 short peptide with immunity reaction on high risk type and low risk type, which can induce and form the antibody for various human papilloma virus (HPV) and coat protein (HPV L1), to check various HPV or HPV L1, while the antibody can be used in biological pharmaceutical engineering, to purify and prepare various HPV or HPV L1.

The invention relates to chimeric molecules comprising a virus coat sequence and a receptor sequence that can inter-act with each other to form a complex that is capable of binding a co-receptor. Such chimeric molecules therefore exhibit functional properties characteristic of a receptor-coat protein complex and are useful as agents that inhibit virus infection of cells due to occupancy of a co-receptor present on the cell. In particular aspects, the chimeric polypeptide includes an immunodeficiency virus envelope polypeptide, such as that of HIV, SIV, FIV, FeLV, FPV and herpes virus. Receptor sequences suitable for use in a chimeric polypeptide include, for example, CD4 D1D2 and CD4M9 sequences.

The present invention relates to one kind of biodegradable medicine-carrying polyglycolide-lactide microsphere in star structure and its preparation process. The biodegradable medicine-carrying polyglycolide-lactide microsphere has coating material of star polyglycolide-lactide, coated protein polypeptide medicine of bovine serum albumin or leuteinizing hormone-releasing homone (LH-RH) analog, medicine carrying amount of 17.56-67.51 mcg / mg microsphere and embedding rate of 28.68-78.39 wt%. It is prepared through a W / O / W composite emulsifying process to coat protein polypeptide medicine into star polyglycolide-lactide to form microsphere. Compared with linear medicine-carrying polyglycolide-lactide microsphere, the present invention has obviously increased medicine carrying amount and embedding rate and obvious long-acting and slowly releasing effect.

The present invention encompasses replication deficient or a replication competent adenoviral vectors which may comprise moieties covering and shielding the vector from the effects of humoral immune responses, as well as a method of constructing and using such vectors. The preferred viral constructs may incorporate the shielding moieties into the pIX coat protein of the adenovirus vectors. The invention also provides recombinant viral vectors with both shielding and specific targeting abilities. Preferably, the viral vector may comprise a nucleic acid sequence, which codes for therapeutically important genes. Methods for treating of a host with an effective amount of adenovirus vector of the present invention are also provided.

The invention provides a herpes virus EBV (Epstein-Barr Virus) detection kit. The kit comprises a nucleic acid releasing agent and PCR (polymerase chain reaction) reaction solution, wherein the nucleic acid releasing agent comprises 0.01-0.5 mM / L of surfactin, 20-300 mM / L of potassium chloride, 0.01-2% of sodium dodecyl sulphate and 0.05-1% of ethanol; and the PCR reaction solution comprises an upstream primer and a downstream primer used for target polynucleotide amplification, and a probe used for target polynucleotide detection. The detection result of the method for releasing nucleic acid by the nucleic acid releasing agent in the kit disclosed by the invention has no obvious difference with the detection result of a boiling method, a strong protein denaturing agent is used during nucleic acid extraction in the kit disclosed by the invention for rapidly breaking the coat protein structure of a pathogen and releasing the nucleic acid of the pathogen, and release and extraction for DNA (deoxyribonucleic acid) can be rapidly finished without heating; the sensitivity of the EBV detection of the kit disclosed by the invention can achieve 400 copies / ml, and the quantitative linear range is 400-4.00E+09 copies / ml; by applying the kit, rapid and accurate detection can be performed on EBV-DNA in the unknown samples of blood plasma, throat swab, peripheral blood and the like, and reliable experimental basis is provided for diagnosing EBV infection.

The invention provides a bran coat antitumor active protein, a preparation method of the protein, and applications of the protein in preparing antitumor drugs and health foods. The preparation method of the protein comprises the steps of pulverizing all natural bran coat, adding protein extracting solution, extracting at low temperature after one night, then adding precooled acetone to precipitate protein at low temperature so as to obtain bran coat protein, adding ammonium sulfate powders with different saturation levels into coarse protein extracting solution for fractional precipitation of the protein, enabling the protein precipitated by the ammonium sulfate to permeate through a Q anion exchange chromatographic column, collecting penetrating fluid, enabling the penetrating fluid to permeate through the Q anion exchange chromatographic column again, performing elution with Tris-NaCl eluant with the concentration of 0.1mol / L, and collecting eluting peaks to obtain the bran coat antitumor active protein (FMB). The protein can obviously restrain the proliferation and the metastasis of colorectal cancer cells and can be applied to preparation of the antitumor drugs and the health foods.

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.

The invention is directed to an adenovirus-antigen conjugate comprising (a) a disrupted adenovirus with a coat protein and (b) an antigen conjugated to the coat protein of the disrupted adenovirus, as well as a conjugate comprising (a) a disrupted adenovirus with a coat protein and (b) an antigen conjugated to the coat protein of the disrupted adenovirus. The invention also provides a method of inducing an immune response against an antigen in a human using the aforementioned conjugates. The invention further provides an adeno-associated viral vector comprising a nucleic acid sequence which encodes an antibody directed against cocaine.

An affinity-conjugated antigen system (ACAS) comprising one or more antigens conjugated via a plurality of affinity moieties to a papaya mosaic virus (PapMV) or virus-like particle (VLP) derived from the coat protein of PapMV is provided. The affinity moieties are molecules or compounds that are capable of specifically binding to the antigen(s) of interest and which can be attached, for example by chemical or genetic means, to the coat protein of the PapMV or PapMV VLP. The ACAS can optionally further comprise one or more additional antigens, which may be the same as, or different to, the conjugated antigen(s) comprised by the ACAS. Also provided are immunogenic compositions, including vaccines, comprising an ACAS. The immunogenic compositions are useful in the treatment, including prevention, of various diseases and disorders for which a humoral and / or cellular response in the animal is required.

The invention discloses preparation, detection and application of a polyclonal antibody of a Yam mild mosaic virus (YMMV). Based on a YMMV genome, the inventor purifies a nuclear inclusion protein / coat protein (NIb / CP) of the YMMV through prokaryotic expression and uses the nuclear inclusion protein / coat protein to prepare the polyclonal antibody for an immune rabbit. The polyclonal antibody can specifically identify the Nib / CP protein of YMMV through prokaryotic expression and the Nib / CP protein of the YMMV virus which infects common yam rhizome, and generates specific immunity response. On the basis, the inventor further builds a set of efficient, high sensitive and accurate IC-RT-PCR (Immunocapture-Reverse Transcription-Polymerase Chain Reaction, ELISA (Enzyme-Linked Immuno Sorbent Assay) and Western blot methods which can specifically detect the YMMV from a common yam rhizome plant which is infected by YMMV. By applying the invention, a material basis and a technical support can be provided for research on interaction of the YMMV and the host, quick detection of the virus and somatotype and molecular biology study, thereby laying a solid foundation for epidemiologically monitoring and preventing treating the virus.

The invention discloses a hybridoma cell strain capable of secreting a tomato yellow leaf curl virus (TYLCV) monoclonal antibody and application of the monoclonal antibody. A coat protein gene of a tomato yellow leaf curl virus separator (TYLCV-SH2) is cloned; the coat protein of the virus is expressed by a prokaryotic expression system; BALB / c mice are immunized by using expression and purification protein as antigen; a hybridoma cell strain D10 capable of performing passage stably and secreting the TYLCV monoclonal antibody is obtained through cell fusion, screening and cloning; and the collection number is CGMCC No.5538. The D10 monoclonal antibody ascites indirect ELISA valence reaches more than 10<-6>; and the antibody type and subclass are IgG1 and kappa chains. A dot-ELISA detection method for detecting the TYLCV of the tomatoes is established by the D10 monoclonal antibody; and when the leaf suffering from the disease is diluted according to the ratio of 1:320 (w / v and g / mL), the virus can still be detected. By the dot-ELISA and Tissue-blot ELISA method, the TYLCV of tomato samples in the field can be detected accurately, specifically and sensitively. Due to establishment of a preparation method for the TYLCV monoclonal antibody and a detection method, technological and material support is provided for diagnosis, prediction and scientific prevention and control of the tomato virus disease.

Herein described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into coat proteins or encapsidation intermediate populations. Each population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different coat proteins or encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different coat proteins such that the reassembled VLP displays more than one peptide or other molecule. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

The invention provides for a composition comprising a genetically engineered bacteriophage capable of guiding cell growth and polarization via signaling peptides and directionally aligned structures. The invention provides for modified bacteriophage and its uses thereof. The present invention also provides for genetically engineered phage capable of guiding cell growth, migration and / or alignment, providing essential biological effects including proliferation and / or differentiation, which can be performed by expressing specific biological motifs, such as the amino acid sequences RGD, IKVAV, DGEA and HPQ, on their coat proteins, on which functional DNA, proteins and cells can be conjugated and / or fixed thereon.

The present invention provides a method of enhancing resistance of plants to one or multiple viruses, comprising introducing to a plant cell, and preferably expressing therein, a nucleotide sequence encoding a virus-encoded polypeptide. The present invention provides a method of enhancing the proportion of virus-resistant or virus-immune lines obtained from a single transformation experiment comprising introducing to a plant cell, a nucleotide sequence encoding a virus-encoded polypeptide operably in connection with a strong promoter sequence. The present invention provides novel gene sequences encoding the coat proteins of a virus and novel dysfunctional replicase sequences as well as gene constructs comprising same, in particular binary vector constructs suitable for introducing into plants and expressing the genes therein. The present invention provides and methods using same to enhance viral resistance in plants. The present invention provides novel methods of testing transgenic plants for the presence of a transgene.

The present invention provides novel technologies for producing and screening fusion proteins on the surface of filamentous phage. In particular, a single vector can be used for generating cell and phage libraries containing a given series of protein sequences fused to either one or other of two phage coat proteins. This approach simplifies and improves the efficiency of the subsequent phage display-based selection of protein-binding molecules having therapeutic or diagnostic utility, such as antibodies, peptides, or epitope-binding regions.

The invention relates to the technical field of biology, in particular to a COVID-19 pseudovirus as well as a preparation method and application thereof. The COVID-19 pseudovirus is formed by virus packaging of coat protein particles and helper plasmids, wherein the coat protein particles comprise plasmids for expressing a COVID-19 S protein, plasmids for expressing a COVID-19 M protein and plasmids for expressing a COVID-19 E protein. The COVID-19 pseudovirus is packaged by adopting a three-plasmid system, and the S / M / E protein is used for replacing expression a VSV-G protein, so that the COVID-19 pseudovirus is stronger in infection capability and higher in sensitivity compared with the pseudovirus only containing the S protein. Moreover, the COVID-19 pseudovirus carries two fluorescentreporter groups, and different fluorescent reporter groups can be applied to different scenes, so that the COVID-19 pseudovirus is simpler and more convenient to apply.

The present invention relates to novel fusion proteins comprising a bacteriophage coat protein and a single-chain T cell receptor and uses of such complexes. In one aspect, the invention relates to soluble fusion protein comprising a bacteriophage coat protein covalently linked to a single-chain T cell receptor which comprises a V-alpha chain covalently linked to a V-beta chain by a peptide linker sequence. The soluble fusion proteins of the invention are useful for a variety of applications including: 1) making a bacteriophage library for displaying single-chain T cell receptors for use in screens for identification and isolation of ligands that bind single-chain T cell receptors, and 2) methods for isolating soluble and fully functional single-chain T cell receptors from the fusion proteins.

The present invention is directed to a DNA construct comprising a first DNA molecule having a length insufficient to independently impart resistance to a virus to plants transformed with said first DNA molecule, wherein the first DNA molecule is from a viral coat protein gene and is at least 110 nucleotides in length. The construct also comprises a second DNA of at least 400 nucleotides in length, which is coupled to the first DNA molecule so that the first and second DNA molecules collectively achieve post-transcriptional silencing and impart resistance to the virus. Alternately, the DNA construct can comprise a plurality of DNA molecules each of which is at least 110 nucleotides in length and from a viral gene, wherein the plurality of DNA molecules are at least 510 nucleotides in length.

The invention relates to a detection method for sugarcane mosaic virus in corn. According to the detection method, a high-quality virus RNA sample is extracted, primers are designed according to the coat protein (CP) genes of the sugarcane mosaic virus, then detection is carried out by utilizing RT-PCR and Real-time RCR technologies, and finally a set of rapid and efficient virus detection method is established. According to the detection method, by effectively detecting the sugarcane mosaic virus with low content in the corn, the potential hazard of the sugarcane mosaic virus is reduced, and the loss of the crop production is reduced.

The invention provides a kit for detecting neisseria gonorrheae (NG). The kit comprises a nucleic acid releaser and a PCR (Polymerase Chain Reaction) solution, wherein the nucleic acid releaser comprises 0.01-0.5mM / L of surfactin, 20-300mM / L of potassium chloride, 0.01-2% of sodium dodecyl sulfate and 0.05-1% of ethanol; and the PCR solution comprises a forward primer and a reverse primer which are used for amplifying targeted polynucleotide, and a probe for detecting the targeted polynucleotide. A method of releasing nucleic acid by using the nucleic acid releaser in the kit provided by the invention is not obviously different from a boiling method in the detection result, and a violent protein denaturant adopted for the nucleic acid extraction in the method provided by the invention quickly breaks a coat protein structure of a pathogen to release pathogen nucleic acid, so the release and extraction of DNA (Deoxyribonucleic Acid) can be realized without heating; besides, the sensitivity of the provided kit for detecting the NG can be 400 copies / ml, the linearity region of the detection is 400-4.00E+10 copies / ml; and moreover, NG-DNA in an unknown sample such as genital secretions can be quickly and precisely detected by using the kit, so a reliable experiment basis is provided for diagnosing NG infection.

The invention belongs to the technical field of plant genetic engineering, and discloses a potyvirus virus-induced gene silencing (VIGS) system with a papaya leaf distortion mosaic virus (PLDMV) as avirus vector. According to the system, a target gene is inserted between Nib and coat protein (CP) of the PLDMV, and a NIa-Pro digestion site is introduced between the target gene and the CP. By in-vitro splicing methods such as Gibson splicing or In-Fusion cloning, target gene fragments are seamlessly cloned and inserted between the Nib and the CP to construct a recombinant virus silence vector containing a plant target gene. Plants are inoculated with the recombinant virus silence vector by agrobacterium injection, and phenotypic changes of the plants are observed after the target gene silencing the plants is induced. The potyvirus VIGS system provides a powerful tool to study gene functions of papaya by using the PLDMV silence vector, and has great scientific significance and application prospects.

The present invention relates to macromolecular complexes comprising micron-scale networks which include binding motifs thereon which allow the covalent bonding of the micron-scale networks to particles which provide nanoscale display surfaces. In particular the present invention relates to micron-scale networks of TMV coat proteins comprising a peptide tag (e.g. SpyTag) and particles providing a nanoscale display surface comprising GFP and a corresponding binding protein (e.g. SpyCatcher) wherein the peptide tag and binding protein pair are capable of spontaneously forming a covalent bond.

The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.

The invention discloses a colloidal gold immunization test strip and a detection method for detecting plum pox viruses. The colloidal gold immunization test strip is formed by the steps of: A. extracting RNA (Ribonucleic Acid) of plum pox viruses from materials infected by the plum pox viruses; B. cloning coat protein genes of the plum pox viruses by an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method, connecting the coat protein genes on a prokaryotic expression vector pET29(a) to obtain pET29a-PPV (porcine parvovirus) recombinant vectors, orderly measuring and finally verifying the recombinant vectors with correct reading frames, carrying out the inducible expression with the coat protein genes of the plum pox viruses, and transforming escherichia coli BL21 (DE3) with the pET29a-PPV recombinant vectors; C. recovering specific expression protein by an affinity column method, and making the rabbits immune to obtain plum pox virus antiserum; and D. adopting the above antiserum to prepare the colloidal gold immunization test strip used for detecting the plum pox viruses by the prepared antiserum. The invention aims to provide the convenient colloidal gold immunization test strip for rapidly detecting the plum pox viruses and the method for detecting plum pox viruses by adopting the test strip.