Detection method for sugarcane mosaic virus in corn
A technology of sugarcane mosaic virus and corn, which is applied in the field of molecular biology detection, can solve the problems of low quality tissue RNA extraction and low plant tissue poisoning, and achieve the effect of reducing losses and reducing potential harm
Inactive Publication Date: 2016-04-20
CHINA AGRI UNIV
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The amount of virus carried by plant tissue is low, and the quality of tissue R...
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[0049] The following examples are used to illustrate the present invention, but not to limit the scope of the present invention. Unless otherwise specified, the examples are in accordance with conventional experimental conditions, such as Sambrook et al. Molecular cloning experimental manual (Sambrook J & Russell DW, Molecular cloning: a laboratory manual, 2001), or according to the conditions recommended by the manufacturer's instructions.
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The invention relates to a detection method for sugarcane mosaic virus in corn. According to the detection method, a high-quality virus RNA sample is extracted, primers are designed according to the coat protein (CP) genes of the sugarcane mosaic virus, then detection is carried out by utilizing RT-PCR and Real-time RCR technologies, and finally a set of rapid and efficient virus detection method is established. According to the detection method, by effectively detecting the sugarcane mosaic virus with low content in the corn, the potential hazard of the sugarcane mosaic virus is reduced, and the loss of the crop production is reduced.
Description
Technical field [0001] The invention relates to the field of molecular biology detection, in particular to RT-PCR and RealTimePCR detection methods of sugarcane mosaic virus in corn. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction) PCR is a molecular biology technology invented by KaryBanksMulis, a scientist at PE-Cetus, USA in 1985 that can rapidly amplify specific genes or DNA sequences in vitro. This technology is based on certain characteristics of rapid replication of DNA sequences in organisms, and realizes rapid amplification of specific DNA sequences in vitro, and can obtain millions of copies of specific DNA sequences in a test tube in a short time. PCR technology is easy to operate and reliable in results. It is widely used in genetic research and analysis in various fields such as medicine, agriculture, archaeology, etc., and has had a revolutionary impact on the development of molecular biology. [0003] The principle of PCR technolog...
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Login to view more IPC IPC(8): C12Q1/70C12Q1/68C12R1/94
Inventor 康定明赵中瑞王宁宁
Owner CHINA AGRI UNIV
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