Peptide and preparation method and application thereof
A technology for inhibiting melanin and sequence, which is applied in the field of genetic engineering, can solve the problems of limited chemical synthesis, limited wide use, and high cost of preparation methods, and achieves the effects of broad market application prospects, correct molecular weight, and low production cost
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Embodiment 1
[0069] Example 1: Construction and expression of pET-32a-M11-2 gene expression vector
[0070] (1) Construction of Escherichia coli Genetic Engineering Bacteria
[0071] 1.1. Since the expression efficiency of the polypeptide can be significantly improved by expressing the M11 polypeptide gene in tandem, this embodiment selects n=2, that is, M11-2 with 2 repeats, and its amino acid sequence is GMPFRWFKPVNGMPFRWFKPVN (SEQ ID No.2).
[0072] 1.2. According to the amino acid sequence of M11-2, the preferred codon gene of Escherichia coli was optimized and selected, that is, ggcatgccgtttcgctggttcaaaccggtgaacggcatgccgtttcgctggtttaaaccggtgaac (SEQ ID No. 6). In order to meet the requirements of subsequent polypeptide digestion, the 5' end of this gene was added with AAT (hydroxyaminase cutting site), and TAA (stop codon) is added to the 3' end, and the complete gene is aat ggcatgccgtttcgctggttcaaaccggtgaacggcatgccgtttcgctggtttaaaccggtgaac taa (SEQ ID No.7) (synthesized by GENEWI...
Embodiment 2
[0084] Example 2: Human Trial of Whitening Short Peptide M11
[0085] The whitening short peptide M11 was sterilized by filtration with a 0.22 μm membrane, diluted with purified water to 40 μg / mL, and stored in the refrigerator for later use.
[0086] 40 volunteers (20 males, 20 females, aged 23-45 years old) were randomly divided into two groups, A and B, 20 in each group (10 males, 10 females), the left face of volunteers in group A was tested 7ml of 40μg / mL whitening short peptide M11, 7ml of pure water was used on the left face of volunteers in group B, and the right face was used as a blank control group. During the experiment period, other whitening cosmetics were stopped, and the melanin content was measured after continuous use for 7 days.
[0087] Method reference: "Cui Huanlian, Cao Rui, Yin Jiazhen, etc. Efficacy Evaluation of Whitening Cosmetics [J]. Fragrance and Fragrance Cosmetics, 2012, 4(2):37-40" (which is incorporated herein by reference), by MX 18 skin p...
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