Method for preparing phase granule for displaying polypeptide

Abstract

This invention involves the phagemid in molecule biology field and medicine field. It is a preparation method of display peptide's phagemid. This method adopts the gene group plasmid of assistant phage fusion protein of coding foreign peptide and pIII coat protein, to co-transformation or successive-transformation F factor positive Isolates together with phagemid, so to obtain phagemid that display foreign peptide. The phagemid prepared by this method has high foreign peptide display level, and it is not necessarily prepare assistant phage so to simplified the process of preparation of assistant phage.

Description

Technical field: [0001] The invention relates to the preparation of phagemid particles in the fields of molecular biology and medicine, in particular to a method for preparing phagemid particles that display polypeptides. Background technique: [0002] Wild-type filamentous phage (filamentous phage) contains a single-stranded closed-loop DNA molecule of approximately 6.4 kb. The virus particle has a diameter of about 6.5 nm and a length of about 900 nm. The outer shell is mainly composed of the main coat protein (pVIII, 2700 copies). There are only 3 to 5 copies of the minor coat protein (pIII). Virus particles can only infect bacterial strains with sexual cilia. When the virus passes through the sexual cilia, the main outer coat protein (pVIII) falls off, and the viral DNA enters the cell and is transformed into double-stranded DNA under the action of intracellular enzymes. [0003] So far, pIII, pVIII, and pVI have all been used to display peptide ligands, protein ligands, anti...

AI Technical Summary

Problems solved by technology

However, we found in our research that if you want to directly use helper phages that express some relatively large proteins such as EGF in the fusion of protein III, the yield of helper phages is very low and the preparation cost is high.

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