Manganese dioxide/glucose oxidase @ hyaluronic acid composite anticancer material, and preparation and application of composite anticancer material

A technology of glucose oxidase and manganese dioxide, which is applied in the field of nanotechnology and cancer treatment, can solve the problems of insufficient selectivity and low anticancer activity, and achieve the effect of good selectivity, low toxicity and side effects, and efficient and precise cancer treatment

Active Publication Date: 2020-02-07
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical deficiencies of low anticancer activity and insufficient selectivity in existing anticancer materials, the present invention provides a manganese dioxide / glucose oxidase@hyaluronic acid composite anticancer material, aiming to provide a solution New material with good stability, excellent selectivity and anticancer activity

Method used

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  • Manganese dioxide/glucose oxidase @ hyaluronic acid composite anticancer material, and preparation and application of composite anticancer material
  • Manganese dioxide/glucose oxidase @ hyaluronic acid composite anticancer material, and preparation and application of composite anticancer material
  • Manganese dioxide/glucose oxidase @ hyaluronic acid composite anticancer material, and preparation and application of composite anticancer material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Synthesis and characterization of TSEN nanomedicine in Example 1

[0046] (1) Take four sets of 500μL manganese dioxide nanosheet original solution (concentration of 0.5mg·mL -1 ; Prepared by the above preparation examples; the following experimental cases, unless otherwise stated, are all solutions of this concentration) and 10μL glucose oxidase solution (5mg·mL) -1 , The following experimental cases, unless otherwise stated, are all solutions of this concentration) mix;

[0047] (2) Then add the above mixture to 1.5, 2, 2.5, 3mL hyaluronic acid solution (2mg·mL -1 ), respectively marked as group 1, group 2, group 3, group 4;

[0048] (3) After magnetic stirring overnight (about 12h) at room temperature, the suspensions were obtained;

[0049] (4) Put the above suspension at 6000r·min -1 Centrifuge at speed for 20 minutes, wash with ultrapure water for 2 to 3 times;

[0050] (5) Measure the hydrated particle size of the four groups of solutions processed by the above steps by Na...

Embodiment 2

[0055] Study on Solution Stability of TSEN Nanomedicine in Example 2

[0056] (1) Add the mixed system of manganese dioxide nanosheets and glucose oxidase (the ratio is the same as in Example 1 step (1)) and the TSEN nano system (prepared in Example 1 step (6)) into DI water. ), physiological saline (Saline), phosphate buffered saline (PBS), cell culture medium containing 10% fetal bovine serum (10% FBS Medium) these four solution systems;

[0057] (2) Among them, the mixed system of manganese dioxide nanosheets and glucose oxidase is set as the Control group;

[0058] (3) Let stand for 30 days at room temperature;

[0059] (4) Observe and record the changes of TSEN in each solution.

[0060] Result analysis: from figure 2 It can be seen from the solution stability test of TSEN that the mixed system of manganese dioxide and glucose oxidase appeared in the first day of standing in each solution, while TSEN can still be stably dispersed in the deionized after standing for 30 days. In w...

Embodiment 3

[0061] Study on the performance of TSEN nanomedicine synergistic production ·OH in Example 3

[0062] Prepare 25mM NaHCO 3 5% CO 2 The buffer solution is ready for use.

[0063] (1) Experimental group A: TSEN nano system (prepared in step (6) of Example 1, 2 mg·mL -1 )Add to with or without 120~150U·mL -1 Hyaluronidase 25mM NaHCO 3 5% CO 2 Buffer solution (containing 10mM GSH, 10mM glucose);

[0064] (2) Experimental group B: Control probe system (composed of manganese dioxide nanosheets and hyaluronic acid), TSEN nano system (2mg·mL -1 ) Were added to 25mM NaHCO 3 5% CO 2 Buffer solution (containing 120~150U·mL -1 Hyaluronidase, 10mM GSH, 10mM glucose);

[0065] (3) Put the above two groups of experimental solution systems A and B to shake at 37°C;

[0066] (4) Centrifuge after 4h, and take the supernatant respectively;

[0067] (5) Add another 10μg·mL -1 Methylene blue (MB) and 50μM H 2 O 2 Were added to the supernatants obtained in the above two experiments;

[0068] (6) Incubate each ...

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Abstract

The invention belongs to the technical field of anticancer drugs, and particularly discloses a manganese dioxide/glucose oxidase @ hyaluronic acid composite anticancer material. The composite anticancer material comprises a core and a shell covering the core, wherein the core comprises a manganese dioxide nanosheet and glucose oxidase modified on the surface of the manganese dioxide nanosheet; andthe shell is hyaluronic acid. The invention further provides preparation and an application of the anticancer material. Studies find that the composite material can effectively and synergistically improve selectivity of cancer cells and normal cells, efficiently and specifically kill the cancer cells, and induce apoptosis of many cancer cells at a low dose through synergy of innovative componentsand composite morphology of the components; and in addition, the material does not influence metabolism of the normal cells basically and has small toxic and side effects.

Description

Technical field [0001] The invention belongs to the field of nanotechnology and cancer treatment, and relates to nano composite drugs based on manganese dioxide nanosheets, glucose oxidase, and hyaluronic acid. Background technique [0002] According to statistics from the World Health Organization in 2015, global cancer deaths reached 8.8 million, and cancer has become the second leading cause of death in the world. To this day, cancer remains a major challenge to public health. Conventional molecular therapies and chemotherapy based on tight ligand-receptor interactions or nucleic acid modification have the limitations of poor stability, poor specificity, and serious side effects. To a large extent, they cannot meet the physical barriers of cancer cells and tumor heterogeneity. The challenges posed by complexity, drug resistance and metastasis. Since the early 1950s, researchers in related fields have successively developed many effective cancer treatment methods, such as che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K33/32A61K38/44A61K31/728A61P35/00
CPCA61K9/5161A61K33/32A61K38/443A61K31/728A61P35/00A61K2300/00
Inventor 卿志和杨荣华柏爱玲
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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