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Duck SBDS (Short Beak and Dwarfism Syndrome) vaccine and preparation method thereof

A syndrome and dwarf technology, applied in the fields of microorganisms and veterinary biopharmaceuticals, can solve the problems of IL-2 research lag, achieve good immune and preventive effects, good safety, and facilitate large-scale production

Inactive Publication Date: 2018-07-27
CHANGCHUN SR BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study of IL-2 in mammals was earlier, but due to species specificity, the study of IL-2 in poultry lags behind

Method used

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  • Duck SBDS (Short Beak and Dwarfism Syndrome) vaccine and preparation method thereof
  • Duck SBDS (Short Beak and Dwarfism Syndrome) vaccine and preparation method thereof
  • Duck SBDS (Short Beak and Dwarfism Syndrome) vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation and identification of goose parvovirus virus-like particles with duck short beak dwarf syndrome

[0024] The VP2 sequence was obtained by sequencing the goose parvovirus M15 strain isolated from the Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, and the VP2 sequence was changed to the classic ATG start. a. Then optimize it according to the preference of Sf9 cells, and send it to the company for gene synthesis. The optimized sequence of duck short beak dwarf syndrome goose parvovirus VP2 is shown in SEQ ID NO.3.

[0025] For the optimized sequence, use primers (shown in SEQ ID NO.4-5) to amplify the VP2 gene, connect it into the pH MCS of the pFastBac Dual vector through PstI-HindIII, and then introduce IL- 2 gene sequence, its nucleotide sequence is shown in SEQ ID NO.6. The IL-VP2 gene was amplified by PCR (primers are as shown in SEQ ID NO.7-8), ligated into p10MCS using SmaI-XhoI, and finally o...

Embodiment 2

[0027] Embodiment 2 Duckling immunity test

[0028] The duck short beak dwarf syndrome goose parvovirus virus-like particles prepared in Example 1 were diluted with PBS so that the latex agglutination titer was 1:2 4 , adding thimerosal at a final concentration of 0.005%, mixing the antigen and oil adjuvant at a ratio of 1:1.5, emulsifying to make a virus-like particle vaccine, and storing at 4°C. Antigen (latex agglutination titer is 1:2) prepared after inactivation of duck embryo allantoic fluid 4 ) and oil adjuvant at a ratio of 1:1.5, emulsified to make a virus-like particle vaccine, and stored at 4°C.

[0029] 18 8-day-old ducklings were randomly divided into 3 groups, with 6 ducks in each group. Group A was immunized with virus-like particle vaccine 0.3ml / feather, group B was immunized with whole virus inactivated vaccine group 0.3ml / feather, and group C was the control Group is not immune. Blood was collected before immunization, 14 days, 21 days, and 28 days after i...

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Abstract

The invention provides a duck SBDS (Short Beak and Dwarfism Syndrome) vaccine and a preparation method thereof. The preparation method is characterized by carrying out fusion expression on an optimized duck SBDS-GPV (Goose Parvovirus) VP2 gene and an interleukin-2 gene, and producing duck SBDS-GPV-like particles by utilizing a baculovirus / insect cell expression system; mixing the duck SBDS-GPV-like particles with a preservative and an adjuvant, thus preparing the duck SBDS vaccine. The duck SBDS vaccine provided by the invention has the advantages of high yield, low production cost, high immunogenicity, good safety, convenience in large-scale production and the like, and has a good immunoprophylaxis effect on duck SBDS.

Description

technical field [0001] The invention relates to the field of microorganisms, genetic engineering and veterinary biopharmaceuticals, in particular to goose parvovirus-like particles of duck short beak dwarf syndrome, its preparation method and its application in the preparation of vaccines. Background technique [0002] Since 2015, there have been outbreaks of Muscovy ducks and Cherry Valley ducks in Fujian, Anhui, Shandong, Henan, Jiangsu, and Zhejiang provinces. A species characterized by soft feet, short bills, exposed tongues, easy fractures, growth disorders, and poor feather growth New disease with clinical characteristics (commonly known as "short-billed duck", "long-tongued duck", "glass duck", "big-tongued duck", etc., tentatively named "duck short beak dwarf syndrome"), the incidence rate is 10% to 100%, The mortality rate is 2% to 10%, and the elimination rate of stiff ducks is 20% to 80%, which causes great economic losses to the duck industry. After epidemiologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/866C07K14/015A61K39/12A61P31/20
CPCA61K39/12C12N15/86C07K14/005C07K14/55A61K2039/55511A61K2039/552A61K2039/5258C07K2319/00C12N2750/14334C12N2750/14323C12N2750/14322C12N2710/14043C12N2800/22C12N2800/105
Inventor 夏振强金宏丽石晶殷玉和刘迪
Owner CHANGCHUN SR BIOLOGICAL TECH
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