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A kind of goose parvovirus strain and application thereof

A technology for goose parvovirus and goose parvovirus disease, which is applied in the directions of antiviral agents, viruses/phages, viral antigen components, etc., can solve problems such as difficult to protect diseases, and achieve good commercial development prospects, good safety, and effectiveness. The effect of immune protection

Active Publication Date: 2020-02-11
SHANDONG SINDER TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the fact that old strains are difficult to protect against current diseases, new mutated virus strains need to be screened to prepare vaccines

Method used

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  • A kind of goose parvovirus strain and application thereof
  • A kind of goose parvovirus strain and application thereof
  • A kind of goose parvovirus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Isolation and identification of embodiment 1GPV-FJ strain

[0017] 1.1 Epidemiological investigation In May 2017, a group of 8-day-old Muscovy ducks in a Muscovy duck farm in Fujian developed respiratory symptoms, which was the initial stage of the disease. Muscovy duck farms were immunized with commercial duck hepatitis bivalent antibody and goose parvovirus inactivated vaccine at 1 day of age. Feet were soft at 8 days of age, and some ducks died. Antibiotics were used, but the improvement did not improve, and the mortality rate increased day by day. Exudative enteritis and sausage-like emboli formed in the intestinal tract were found to be the main pathological changes in the dead ducks. Systemic dehydration myocardial congestion, liver and spleen enlargement, etc. The laboratory diagnosis was a mutant strain of goose parvovirus.

[0018] 1.2 Virus Isolation Take 50-100 g of diseased tissues such as the brain, viscera, and intestinal tract of the sick white muscovy ...

Embodiment 2

[0039] Embodiment 2 Vaccine manufacture and semi-finished product inspection

[0040] 2.1 Virus seed preparation of GPV-FJ strain The allantoic fluid (the 3rd generation of the original virus seed of GPV-FJ strain) is made 500-fold dilution with sterilized PBS, and inoculates 10 Muscovy duck embryos of 12 days old through the allantoic cavity, each Embryo 0.2ml, place 36~38 ℃ to continue to incubate, select the duck embryo that dies within 48~144 hours after inoculation. The allantoic fluid was collected and centrifuged, and the supernatant was taken for passage to the next generation. According to this method, 10 generations were passed continuously, which were respectively marked as C3-C10 generations. The virus content of each passage was determined. The same generation will be tested for sterility and virus content ≥ 10 5.0 ELD 50 / 0.2ml virus solution, quantitatively aliquoted, freeze-dried and stored.

[0041] 2.2 Preparation of GPV-FJ strain antigen Dilute the viru...

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Abstract

The invention provides a goose parvovirus strain with preservation number of CCTCC NO:V201811. The goose parvovirus strain can be used for preparing a vaccine for preventing goose parvovirus derived from muscovy duck. A prepared inactivated vaccine for the goose parvovirus is good in safety, and local or general adverse reactions caused by the vaccine are avoided. Analysis of characters, safety testing and potency testing data in storage life tests proves that various indexes are stable and effective; the immune effect of the vaccine is evaluated with a serological method and an immune challenge method, and results show that the prepared inactivated vaccine can provide effective immune protection for muscovy ducks and has good commercial development prospect.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a goose parvovirus strain and application thereof. Background technique [0002] Goose parvovirus infection, also known as gosling plague, is an acute infectious disease caused by goose parvovirus, which mainly affects goslings and young Muscovy ducks aged 1 to 3 weeks, with exudative enteritis as the main pathological change , acute onset, high mortality, and rapid spread are the characteristics. The morbidity and mortality rate decrease with the increase of the age. It mainly affects goslings and muscovy ducks less than 7 days old, and the mortality rate can reach 100%. The mortality rate of those over 10 days old generally does not exceed 60%. The incidence rate in the older age group is low. According to the incidence, it is divided into 3 types, the most acute type, the acute type and the subacute type. In 1974, the World Poultry Society...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/23A61P31/20A61K41/00A61L2/18C12N7/00C12R1/93
CPCA61K39/12A61K41/10A61K2039/5252A61L2/0088A61L2202/21A61P31/20
Inventor 王相芹王丹娜谭鹏王学波林文超李艳丽李朝阳
Owner SHANDONG SINDER TECH
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