44 results about "Parvovirus infection" patented technology

An exemplary embodiment providing one or more improvements includes feeding animals with probiotic microbes encapsulated in a mixture of xanthan gum and chitosan, or in gelatin, specifically Pediococcus acidilactici and Saccharomyces boulardii. Such encapsulation protects the viability of the probiotic microbes against unfavorable temperatures. An exemplary embodiment providing one or more improvements includes methods of using viable probiotics in therapy of birds and mammals infected with infectious diseases. Probiotics acted as adjuvants in stimulating antibody reaction and stimulated a cellular immunity response. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

An exemplary embodiment providing one or more improvements includes feeding animals with probiotic microbes encapsulated in a mixture of xanthan gum and chitosan, or in gelatin, specifically Pediococcus acidilactici and Saccharomyces boulardii. Such encapsulation protects the viability of the probiotic microbes against unfavorable temperatures. An exemplary embodiment providing one or more improvements includes methods of using viable probiotics in therapy of birds and mammals infected with infectious diseases. Probiotics acted as adjuvants in stimulating antibody reaction and stimulated a cellular immunity response. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

A novel representational system that is a method for improving feeding probiotics is provided. The probiotics, especially the Pediococcus acidilactici and the Saccharomyces boulardii are mixed in different colloid net including xanthan, chitosan or gelatin. By using said mixed colloid the activity of the probiotics can be protected, and multiple execrable surroundings and disadvantageous temperature for growth can be prevented. The novel representational method also provides many kinds of method for treating infectious disease for birds and mammals by using activated probiotics. Furthermore, the probiotics is usually acted as adjuvants for improving the generation of antibody and the cellularity immunity effect. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

The invention provides a hybridoma prepared from canine parvovirus antigens. An anti-canine-parvovirus monoclonal antibody secreted from the hybridoma has quite high relative affinity constant and good neutralizing activity, and a vaccine composition prepared from the monoclonal antibody can effectively prevent and treat canine parvovirus infections. A canine parvovirus detection kit prepared with two kinds of monoclonal antibodies has the advantages of quickness, convenience, accuracy, good sensitivity, specificity and repeatability and has higher sensitivity than colloidal gold-labeled commercial test strips.

The invention aims to provide a Muscovy duck parvovirus. The Muscovy duck parvovirus is a YBMDV strain, and is preserved in the China General Microbiological Culture Collection Center of the China Committee Of Culture Collection For Microorganisms in the Institute of Microbiology Chinese Academy of Science at the address of No. 3, No. 1 yard, Beichen west road, Chaoyang District, Beijing, on November 11th, 2013; the preservation number is CGMCC No. 8504. The Muscovy duck parvovirus screened by the invention has the characteristics of low toxicity and high immunogenicity; dead embryo and embryo liquid can be obtained by vaccinating the Muscovy duck parvovirus to a Muscovy duck embryo; virus liquid can be collected after grinding and freeze thawing; a vaccine is prepared by ultrafiltration concentration, formalin inactivation, the addition of an adjuvant, and mixing and emulsification; the Muscovy duck parvovirus can improve antibody of the Muscovy duck, ensure parent source antibody level of an offspring, and prevent green duck parvovirus infection caused by Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention relates to the technical field of biological products, in particular to an egg yolk antibody for preventing and controlling a duck parvovirus and a preparation method thereof. The egg yolk antibody is derived from primiparity eggs of healthy laying hens which are processed through high-intensity combined immunization by using duck parvovirus live vaccines and duck parvovirus inactivated vaccines. According to the egg yolk antibody, the preparation method is easy to operate and suitable for mass production, and the prepared duck parvovirus egg yolk antibody is stable in property, high in purity, potency and specificity, free of drug residues, mild and environmentally friendly, extremely has the application value on prevention and treatment of duck parvovirus infection and supplies a new way to preparation of the duck parvovirus egg yolk antibody.

The invention discloses a preparing method of anti-dog parvoviral vitelline antibody biological preparation, which comprises the following steps: collecting disease material of dead dog with parvoviral infection; centrifuging; removing depositing slag; centrifuging the supernatant fluid; adding into chloroform; vibrating severely; centrifuging; getting the supernatant fluid; aldehyde-washing pig red cell; adding the supernatant into the pig red cell; vibrating evenly; stewing; centrifuging; getting depositing liquid; adding into salt water; stewing; centrifuging; getting virus liberating liquid; testing HA potency with liberating liquid; reaching 1: 4096 effective; deactivating the virus liberating liquid with methyl aldehyde; adding into oil emulsion dog parvoviral vaccine prepared by brown algae polysaccharide; passing the vaccine to hen after delivery; testing HI potency of immune ovum with hemagglutination inhibition test; harvesting vitelline after reaching standard; diluting with sterile distilled water; proceeding acid treatment with lactic acid liquid; removing mixed protein; degreasing with freeze-thaw; salting-out suction liquid; adjusting IgY density; loading; putting into bathing box; treating; getting the oral biological preparation.

The invention aims to provide an egg yolk antibody for preventing and curing muscovy duck parvovirus diseases, and the egg yolk antibody is prepared by using muscovy duck parvoviruses the preserving number of which is CGMCC (China General Microbiological Culture Collection Center) No.8504 as an antigen. The egg yolk antibody is characterized in that a muscovy duck parvoviruse YBMDP plant is inoculated with a muscovy duck embryo, a die embryo body and embryo liquid are respectively obtained and are grinded and are subjected to freeze thawing so as to obtain a virus solution, and the oil adjuvant mixing emulsification is added in the virus solution after ultrafiltration concentration and formaldehyde solution inactivation so as to obtain a vaccine. The vaccine is utilized to inoculate a laying hen, eggs are obtained, yolks are separated, and the antibody is obtained after inactivation and extraction and rectification. The prepared antibody can prevent ducklings from the parvovirus infection because of muscovy duck parvoviruses, and the egg yolk antibody has the advantages of high efficiency, good safety, high protection rate and the like.

The invention provides a Muscovy duck parvovirus inactivation vaccine which comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated Muscovy duck parvovirus, and the collection number of the Muscovy duck parvovirus is CGMCC No.8504. The Muscovy duck parvovirus inactivation vaccine provided by the invention is prepared by the steps of screening a Muscovy duck parvovirus YBMDP strain with low toxicity and high immunogenicity; inoculating and collecting the virus liquid; performing ultrafiltration concentration and formaldehyde solution inactivation; adding an oil adjuvant and mixing and emulsifying to obtain the vaccine. The vaccine provided by the invention can improve the antibody level of Muscovy duck, guarantee the maternal antibody level of the filial generation and prevent the duckling parvovirus infection caused by the Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention provides a codon-optimized parvovirus polynucleotide composition and methods of expressing this polynucleotide in a variety of mammalian cells, including non-erythroid progenitor cells, to produce immunogenic compositions.

The invention relates to the field of animal virology and provides a duck parvovirus strain, live vaccine thereof and a preparing method. The strain is a duck parvovirus and specifically named GT2015v, and the biology preservation serial number of the strain is CCTCC No:V201528; the vaccine prepared by utilizing the strain effectively solves the problem that the disease can not be prevented at present in China, by means of continuous passage of duck embryos, a medium virulence isolate strain is attenuated and made to lose pathogenicity to ducks, good immunogenicity is kept, duck parvovirus infection can be prevented and controlled safely and effectively, cultivation risks are reduced, and sound development of duck breeding industry is guaranteed.

The present invention provides a method for diagnosing a subject suffering a pathological condition characterized by parvovirus infection by detecting the presence of IgE anti-Parvovirus B 19 antibodies.

The invention relates to a traditional Chinese medicine composition for treatment for canine parvovirus infection, which is characterized in that the traditional Chinese medicine composition is prepared by the components with the following parts by weight by mixing: 12 to 18 parts of garden burnet root, 8 to 12 parts of golden thread, 8 to 12 parts of peach seed, 8 to 12 parts of whiteflower hogfennel root and 8 to 12 parts of membranous milkvetch root. The preferential mix ratio of the all components is as follows: 15 parts of garden burnet root, 10 parts of golden thread, 10 parts of peach seed, 10 parts of whiteflower hogfennel root and 10 parts of membranous milkvetch root. The preparation method is carried out according to the following steps: (1) the all ingredients in the formula are respectively smashed and screened by a sieve with 20 to 80 meshes; (2) 15 parts of garden burnet root, 10 parts of golden thread, 10 parts of peach seed, 10 parts of whiteflower hogfennel root and 10 parts of membranous milkvetch root are weighed according to the mix ratio by the parts by weight for even mixing. The traditional Chinese medicine composition has reasonable formula and simple preparation; the pure traditional Chinese medicine composition has no pollution, no medicine residue, low cost, broad material selection and very significant efficacy. The traditional Chinese medicine composition selects a plurality of pure traditional Chinese medicine preparations, thus having unique advantages compared with western medicines. The clinical application proves that the traditional Chinese medicine composition has special effects on canine parvovirus infection.

The present invention relates to the discovery of a new human parvovirus, methods of detecting the parvovirus and diagnosing parvovirus infection, methods of treating or preventing parvovirus infection, and methods for identifying anti-parvoviral compounds.

The invention discloses a pig parvovirus immune composition which contains the following a) and b), or is basically prepared from the following a) and b), or is prepared from the following a) and b):a) viroid particles formed by self-assembly of pig parvovirus VP2 protein expressed by Kluyveromyces marxianus recombinant bacterium; b) pharmaceutically or veterinarily acceptable accessories used for injection preparations. The invention also discloses a preparation method and application of the pig parvovirus immune composition. Pig parvovirus viroid particle vaccine injection immunization canobtain high-level serum IgG antibodies, has good immunogenicity and can reduce and prevent related diseases caused by pig parvovirus infection.

The invention relates to a vaccine composition comprising an immunizing dose of a canine parvovirus antigen and an immunizing dose of another antigen, and relates to a kit comprising an effective amount of the canine parvovirus antigen and an effective amount of another antigen. The composition and the kit can be effectively used for preparation of medicines preventing and / or treating canine parvovirus infection related diseases. The composition and the kit can effectively prevent present epidemic canine parvovirus wild strains, and synergistic effects with some antigens in other antigens aregenerated, thus further improving immunization effects of a combined vaccine.

The invention discloses a preparation method of porcine parvovirus genetic engineering vaccine, which comprises the following steps: constructing and recombining a yeast expression carrier p GAPZ alpha-VP2; then carrying out electric shock to convert pichia pastoris SMD1168, so that VP2 protein is efficiently secreted and expressed in a YPD culture medium to form viruslike particles without virusnucleic acid; purifying protein, carrying out SDS-PAGE and Western Blot and electric microscope detection; and adding a corresponding immunologic adjuvant to prepare the porcine parvovirus genetic engineering vaccine. The porcine parvovirus genetic engineering vaccine prepared by the invention solves the defects in the current porcine parvovirus genetic engineering vaccine, can be used for preventing and treating porcine parvovirus genetic infection and relative diseases caused by the porcine parvovirus genetic infection, and is also suitable for preparing and detecting the envelope antigen of a porcine parvovirus antibody ELISA kit.

The invention discloses a pure traditional Chinese medicine formula for canine parvovirus infection. The raw materials of the formula include the following components in parts by weight: 30-40 parts of Pulsatilla chinensis, 15-20 parts of reed rhizome carbon, and 12-16 parts of Cortex Phellodendri . 5-15 parts of ginger; widely used materials, low cost, safe and effective, no drug resistance, easy to use, has the functions of clearing heat and detoxifying, clearing liver and improving eyesight, moistening lung and reducing phlegm, cooling blood and stopping dysentery, quenching wind and relieving spasm, promoting clearness and reducing turbidity 1. The effect of enhancing the immune function of the body. The composition was used for experimental treatment of dogs, foxes and raccoon dogs suffering from canine parvovirus, and more than a thousand dogs were cured, with a cure rate of more than 85%. The curative effect is remarkable.

The present invention is directed to mutant parvovirus VP1 unique region polypeptides, compositions comprising such polypeptides, methods of making such compositions, as well as methods for identifying the likely presence of parvovirus-neutralizing antibodies, and methods for assessing the functional immunogenicity of parvovirus vaccines and measuring a correlate of efficacy to assess a treatment for parvovirus infection.

The present invention relates to compounds for use in a method for the treatment or prevention of parvovirus infections in humans and warm-blooded animals, including feline panleukopenia virus (FPV) infections in felids, canine parvovirus type 2 (CPV-2) infections in canines, minute virus of mice (MVM) infections in mice and B19 parvovirus infections in humans.

The present invention relates to the discovery of a new human parvovirus, methods of detecting the parvovirus and diagnosing parvovirus infection, methods of treating or preventing parvovirus infection, and methods for identifying anti-parvoviral compounds.

The invention discloses a traditional Chinese medicinal composition for treating mink diarrhea and a preparation method of the traditional Chinese medicinal composition, and belongs to the field of traditional Chinese medicine treatment of mink diarrhea. The traditional Chinese medicinal composition for treating mink diarrhea disclosed by the invention is prepared from the following various medicinal raw materials: loquat leaf, blackberrylily rhizome, swamp mahogany leaf, radix sophorae flavescentis, caulis akebiae, coix seed, mirabilite, ligusticum wallichii and fresh ginger. The invention further discloses a method for preparing the traditional Chinese medicinal composition, wherein the method comprises the following steps: (1) weighing the various medicinal raw materials according to the proportions; (2) adding water and soaking the raw materials, decocting, filtering, removing dregs and reserving filtrate so as to obtain the traditional Chinese medicinal composition. The traditional Chinese medicinal composition disclosed by the invention has functions of clearing away heat and toxic materials, cooling blood and stopping diarrhea, relieving diarrhea with astringents and invigorating qi and blood; the traditional Chinese medicinal composition is capable of significantly enhancing the resistance and immunity of minks and effectively resisting mink diarrhea caused by parvovirus infection or mixed infection of various bacteria; and the traditional Chinese medicinal composition is high in cure rate, free from reoccurrence after curing, free from influence on the quality of mink fur and free from any toxic or side effect.

The invention discloses canine parvovirus vaccine composition injection preparation and oral preparation. The canine parvovirus vaccine composition injection preparation and oral preparation contain the following (a) and (b), and basically comprise the following (a) and (b), or comprise the following (a) and (b): (a) virus-like particle formed by self-assembling canine parvovirus VP2 protein expressed kluyveromyces marxianus recombinant bacterium; (b) an auxiliary material which is acceptable in terms of medicine or veterinary medicine and used for an injection / oral preparation. The inventionfurther discloses a preparation method and application of the vaccine composition. The canine parvovirus virus particle vaccine can obtain high-level serum IgG antibody after immunization, has excellent immunogenicity, can reduce and prevent relevant diseases caused by canine parvovirus infection, has the advantages of high safety, low production cost, capability of enlarging scale production andhigh convenience in operation, and can be applied to the prevention and reducing of relevant clinical symptoms caused by CPV infection.

The invention relates to a vaccine composition containing an immune amount of canine parvovirus antigen and an immune amount of other antigens, and also relates to a kit containing an effective amount of canine parvovirus antigen and an effective amount of other antigens, and the prepared vaccine composition Both the method and the kit can be effectively used for preparing medicines for preventing and / or treating diseases related to canine parvovirus infection. The vaccine composition and kit of the invention can effectively prevent the existing wild strains of the circulating canine parvovirus, and have a synergistic effect with some antigens in other antigens to further improve the immune effect of the combined vaccine.

The invention provides a Muscovy duck parvovirus inactivation vaccine which comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated Muscovy duck parvovirus, and the collection number of the Muscovy duck parvovirus is CGMCC No.8504. The Muscovy duck parvovirus inactivation vaccine provided by the invention is prepared by the steps of screening a Muscovy duck parvovirus YBMDP strain with low toxicity and high immunogenicity; inoculating and collecting the virus liquid; performing ultrafiltration concentration and formaldehyde solution inactivation; adding an oil adjuvant and mixing and emulsifying to obtain the vaccine. The vaccine provided by the invention can improve the antibody level of Muscovy duck, guarantee the maternal antibody level of the filial generation and prevent the duckling parvovirus infection caused by the Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention relates to the technical field of biological products, in particular to an egg yolk antibody for preventing and controlling a duck parvovirus and a preparation method thereof. The egg yolk antibody is derived from primiparity eggs of healthy laying hens which are processed through high-intensity combined immunization by using duck parvovirus live vaccines and duck parvovirus inactivated vaccines. According to the egg yolk antibody, the preparation method is easy to operate and suitable for mass production, and the prepared duck parvovirus egg yolk antibody is stable in property, high in purity, potency and specificity, free of drug residues, mild and environmentally friendly, extremely has the application value on prevention and treatment of duck parvovirus infection and supplies a new way to preparation of the duck parvovirus egg yolk antibody.

The present disclosure relates to a novel murine parvovirus, sequences encoded thereby, and applications therefor. In one embodiment the disclosure provides a method for detecting the presence of a parvovirus in a sample, comprising detecting one or more nucleic acids or polypeptides derived from the parvovirus, or antibodies against the parvovirus, in the sample. Also provided are vectors and host cells comprising sequences encoded by the parvovirus and related sequences. Also provided are animal models of kidney disease associated with infection by the parvovirus.

The invention aims to provide a Muscovy duck parvovirus. The Muscovy duck parvovirus is a YBMDV strain, and is preserved in the China General Microbiological Culture Collection Center of the China Committee Of Culture Collection For Microorganisms in the Institute of Microbiology Chinese Academy of Science at the address of No. 3, No. 1 yard, Beichen west road, Chaoyang District, Beijing, on November 11th, 2013; the preservation number is CGMCC No. 8504. The Muscovy duck parvovirus screened by the invention has the characteristics of low toxicity and high immunogenicity; dead embryo and embryo liquid can be obtained by vaccinating the Muscovy duck parvovirus to a Muscovy duck embryo; virus liquid can be collected after grinding and freeze thawing; a vaccine is prepared by ultrafiltration concentration, formalin inactivation, the addition of an adjuvant, and mixing and emulsification; the Muscovy duck parvovirus can improve antibody of the Muscovy duck, ensure parent source antibody level of an offspring, and prevent green duck parvovirus infection caused by Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention relates to the field of animal virology, and provides a duck parvovirus strain and a live vaccine and a preparation method thereof. The strain is duck parvovirus, specifically named GT2015v strain, and its biological preservation number is CCTCC No: V201528; The vaccine prepared by this strain effectively solves the current situation that the disease cannot be prevented in our country. The medium-virulence isolates are weakened through continuous passage of duck embryos, so that they lose their pathogenicity to ducks and maintain good immunity. It can safely and effectively prevent and control duck parvovirus infection, reduce the risk of breeding, and ensure the healthy development of the duck industry.