Muscovy duck parvovirus inactivation vaccine and application thereof

A technology of Muscovy duck parvovirus and inactivated vaccine, which is applied in the directions of antiviral agents, medical preparations containing active ingredients, and antibody medical ingredients, etc. Effects of parvovirus infection

Active Publication Date: 2014-06-04
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, due to the reduction of the potency of the vaccine used due to virus mutation, it is also necessary to screen out the mutated virus strain to prepare a vaccine with higher efficacy

Method used

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  • Muscovy duck parvovirus inactivation vaccine and application thereof
  • Muscovy duck parvovirus inactivation vaccine and application thereof
  • Muscovy duck parvovirus inactivation vaccine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the screening of YBMDV strain

[0015] In 2011, acute muscovy duck parvovirus disease broke out from a duck farm in Zhejiang Province. The pancreas and intestines of the dead ducks were taken, and the pancreas and intestinal tissues were homogenized with non-normal saline to make a 20% suspension, 3000r / Centrifuge for 15 minutes, take the cleared bacteria, and inoculate 13-day-old muscovy duck embryos through the allantoic cavity, hatch for 120 hours, collect the allantoic fluid and embryo body tissue, homogenize, freeze and thaw three times repeatedly, and take the supernatant Freeze. After the harvested virus liquid was purified, the virus characteristics of the virus content, immunogenicity, specificity and purity were analyzed and tested, and the results showed that the virus content of the strain was 10 5.7 ELD 50 / 0.2ml, the minimum immune dose is 10 2.0 ELD 50 / 0.2ml, the virus only reacts specifically with Muscovy duck parvovirus, without bact...

Embodiment 2

[0019] Embodiment 2 prepares vaccine

[0020] 1. Preparation of virus liquid for seedling production Inoculate 12-14-day-old susceptible muscovy duck embryos into the allantoic cavity of the virus species YBMDV strain for production, 0.2ml per embryo, incubate at 36-37°C, and inspect the embryos twice a day . Select embryos that died within 48 to 120 hours after inoculation, and embryo bodies with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and mild edema of choriourinium, and put the allantoic fluid and embryo bodies into a tissue masher Crush, take the crushed tissue and mix it with saline at a ratio of 1:2; place it at 2-8°C for 4-12 hours, collect allantoic fluid, amniotic fluid and embryo body (remove the head and limbs). Homogenize with embryo fluid, freeze-thaw 3 times, centrifuge at 4000r / min for 30min, take supernatant and mix it in a sterile container, store at 2-8°C.

[0021] 2. Inactivation Put the YBMDV strain virus liquid in...

Embodiment 3

[0044] 1. Preparation of antigens for seedling production

[0045] 1. Preparation of virus liquid for seedling production Inoculate 12-14-day-old susceptible muscovy duck embryos into the allantoic cavity of the virus species YBMDV strain for production, 0.2ml per embryo, incubate at 36-37°C, and inspect the embryos twice a day . Muscovy duck embryos that died within 48 to 120 hours after inoculation, and embryo bodies with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantoic membrane with mild edema were selected, and the allantoic fluid and Muscovy duck embryo bodies were put into Crush the tissue in a masher, take the crushed tissue and mix it with normal saline at a ratio of 1:2; place it at 2-8°C for 4-12 hours, and collect the allantoic fluid, amniotic fluid, and embryo body (remove the head and limbs). Homogenize with embryo fluid, freeze-thaw 3 times, centrifuge at 4000r / min for 30min, take supernatant and mix it in a st...

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Abstract

The invention provides a Muscovy duck parvovirus inactivation vaccine which comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated Muscovy duck parvovirus, and the collection number of the Muscovy duck parvovirus is CGMCC No.8504. The Muscovy duck parvovirus inactivation vaccine provided by the invention is prepared by the steps of screening a Muscovy duck parvovirus YBMDP strain with low toxicity and high immunogenicity; inoculating and collecting the virus liquid; performing ultrafiltration concentration and formaldehyde solution inactivation; adding an oil adjuvant and mixing and emulsifying to obtain the vaccine. The vaccine provided by the invention can improve the antibody level of Muscovy duck, guarantee the maternal antibody level of the filial generation and prevent the duckling parvovirus infection caused by the Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

Description

technical field [0001] The invention belongs to the technical field of poultry pathogenic microorganism screening, and in particular relates to an inactivated Muscovy duck parvovirus vaccine and its application. Background technique [0002] Muscovy duck parvovirus disease is an acute or subacute infectious disease that affects young muscovy ducks. It is an infectious disease mainly characterized by panting, diarrhea, pancreatic necrosis and hemorrhage; it is highly contagious, and its morbidity and mortality are high. Very high, often causing serious economic losses. The disease mainly occurs in young muscovy ducks of 1 to 3 weeks of age, especially young muscovy ducks of about 10 days of age. The disease first appeared in Fujian, China, and the Brittany region of western France in the mid-to-late 1980s, and it was recognized as an independent disease different from gosling plague in the early 1990s. If the dead muscovy ducks are not handled properly, the virus will often...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/23A61P31/20
Inventor 李陆梅宫晓徐保娟王红宋新宇邹敏李芳
Owner YEBIO BIOENG OF QINGDAO
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