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A duck parvovirus strain and its live vaccine

A duck parvovirus and live vaccine technology, applied in the field of animal virology, can solve the problems of inability to use vaccines for prevention and control, low potency, etc., achieve good immunogenicity and safety, ensure healthy development, and reduce breeding risks

Active Publication Date: 2018-04-03
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have confirmed that the neutralization titer of the virus and goose parvovirus and muscovy duck parvovirus antibodies is low, and the vaccines for the above two viruses cannot be used for the prevention and control of the disease

Method used

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  • A duck parvovirus strain and its live vaccine
  • A duck parvovirus strain and its live vaccine
  • A duck parvovirus strain and its live vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and identification of GT2015 virus

[0033] In April 2015, the affected ducks collected from the Gaotang area of ​​Shandong were clinically manifested as growth retardation and atrophy of the upper and lower beaks. Isolate and identify the isolated strains by PCR, EID50 toxicity test, animal regression test, etc. The results showed that the target fragment was successfully amplified; the virus virulence was 10 6.7 EID50 / 0.2mL; The inoculated ducks in the animal infection test had obvious clinical symptoms and pathological changes, which were consistent with the clinical diseased ducks. Therefore, it was determined that the isolated strain was duck parvovirus, and it was named GT2015 strain, and the inventor carried out a biological deposit on it, and the deposit number was CCTCC No: V201527.

Embodiment 2

[0034] Example 2 Obtaining of GT2015v virus and identification of its biological characteristics

[0035] Acquisition of GT2015v virus

[0036] The duck parvovirus GT2015 strain was inoculated into 9-day-old duck embryos for 40 generations, and the attenuated strain was cloned and purified, which was named GT2015v strain. Virus titer was measured as 10 6.6 EID50 / 0.2mL. It shows that the attenuated strain has good reproduction ability in duck embryo. The inventor has carried out a biological deposit on it, and the depository unit is the China Center for Type Culture Collection, and the biological deposit number is CCTCC No: V201528.

[0037] Biological characteristics of duck parvovirus GT2015v

[0038] 1 Safety test: 100 1-day-old ducklings were divided into 5 groups on average, and 10 6 F10, F20, F30 and F40 generation strains of EID50, and set a blank control group. Symptoms were observed and weighed for 25 consecutive days. Every five days, two animals in each group ...

Embodiment 3

[0046] Embodiment 3 Vaccine finished product preparation

[0047] Nine-day-old duck embryos were inoculated with the attenuated GT2015v strain of duck parvovirus, incubated at 37°C for 5 days, and the virus allantoic fluid was harvested, added with lyoprotectant, and vacuum freeze-dried to prepare duck parvovirus live vaccine.

[0048] The preparation method of the duck parvovirus live vaccine provided by the invention comprises inoculating duck embryos with duck parvovirus attenuated GT2015v strain, harvesting virus cultures, adding a stabilizing protective agent, and drying in vacuum.

[0049] Propagation of vaccine strains: Well-developed 9-day-old healthy duck embryos were selected for virus propagation, and the virus solution of the attenuated GT2015v strain of duck parvovirus was inoculated at 0.2 mL / embryo, cultured at 37°C for 5 days, and the virus allantoic fluid was collected and stored at -20°C save.

[0050]Inspection of semi-finished products:

[0051] Sterility...

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Abstract

The invention relates to the field of animal virology, and provides a duck parvovirus strain and a live vaccine and a preparation method thereof. The strain is duck parvovirus, specifically named GT2015v strain, and its biological preservation number is CCTCC No: V201528; The vaccine prepared by this strain effectively solves the current situation that the disease cannot be prevented in our country. The medium-virulence isolates are weakened through continuous passage of duck embryos, so that they lose their pathogenicity to ducks and maintain good immunity. It can safely and effectively prevent and control duck parvovirus infection, reduce the risk of breeding, and ensure the healthy development of the duck industry.

Description

technical field [0001] The invention relates to the field of animal virology and provides a duck parvovirus strain and a live vaccine thereof. Background technique [0002] Duck parvovirus is a kind of parvovirus, which mainly affects ducklings aged 1 to 3 weeks. The main clinical symptoms are diarrhea, dyspnea and soft feet. It occurs all year round and brings great economic benefits to duck breeding. Loss is one of the main diseases in duck breeding, and the morbidity and mortality are also high. [0003] Since March 2015, a disease has appeared in meat ducks in Shandong, Jiangsu, Anhui and other places in my country. It has been verified as duck parvovirus. The disease is characterized by dysplasia of duck beak and protruding tongue. For commercial ducks over the age of one day, the incidence rate of duck flocks is 10%-20%, up to 50%, and the infection rate of large flocks can reach up to 100%, but the disease does not cause the death of ducks. Affected ducks are stunted...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/23A61P31/20C12R1/93
Inventor 刁有祥陈浩于相龙窦砚国郑肖强牛晓宇杨晶
Owner SHANDONG AGRICULTURAL UNIVERSITY
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