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Muscovy duck parvovirus and application thereof

A technology of Muscovy duck parvovirus and microbial strains, applied in antiviral agents, virus/bacteriophage, antiviral immunoglobulin, etc., can solve the problems of reduced vaccine potency, achieve high immunogenicity, low toxicity, prevent Effects of parvovirus infection in ducklings

Active Publication Date: 2014-05-28
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, due to the reduction of the potency of the vaccine used due to virus mutation, it is also necessary to screen out the mutated virus strain to prepare a vaccine with higher efficacy

Method used

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  • Muscovy duck parvovirus and application thereof
  • Muscovy duck parvovirus and application thereof
  • Muscovy duck parvovirus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0014] 2. Preparation of antigens for seedling production

[0015] 1. Preparation of virus liquid for seedling production Inoculate 12-14-day-old susceptible muscovy duck embryos into the allantoic cavity of the virus species YBMDV strain for production, 0.2ml per embryo, incubate at 36-37°C, and inspect the embryos twice a day . Select embryos that died within 48 to 120 hours after inoculation, and embryo bodies with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and mild edema of choriourinium, and put the allantoic fluid and embryo bodies into a tissue masher Crush, take the crushed tissue and mix it with saline at a ratio of 1:2; place it at 2-8°C for 4-12 hours, collect allantoic fluid, amniotic fluid and embryo body (remove the head and limbs). Homogenize with embryo fluid, freeze-thaw 3 times, centrifuge at 4000r / min for 30min, take supernatant and mix it in a sterile container, store at 2-8°C.

[0016] 2. Inactivation Put the YBMDV s...

Embodiment 1

[0039]1. Preparation of antigens for seedling production

[0040] 1. Preparation of virus liquid for seedling production Inoculate 12-14-day-old susceptible muscovy duck embryos into the allantoic cavity of the virus species YBMDV strain for production, 0.2ml per embryo, incubate at 36-37°C, and inspect the embryos twice a day . Muscovy duck embryos that died within 48 to 120 hours after inoculation, and embryo bodies with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantoic membrane with mild edema were selected, and the allantoic fluid and Muscovy duck embryo bodies were put into Crush the tissue in a masher, take the crushed tissue and mix it with normal saline at a ratio of 1:2; place it at 2-8°C for 4-12 hours, and collect the allantoic fluid, amniotic fluid, and embryo body (remove the head and limbs). Homogenize with embryo fluid, freeze-thaw 3 times, centrifuge at 4000r / min for 30min, take supernatant and mix it in a ste...

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Abstract

The invention aims to provide a Muscovy duck parvovirus. The Muscovy duck parvovirus is a YBMDV strain, and is preserved in the China General Microbiological Culture Collection Center of the China Committee Of Culture Collection For Microorganisms in the Institute of Microbiology Chinese Academy of Science at the address of No. 3, No. 1 yard, Beichen west road, Chaoyang District, Beijing, on November 11th, 2013; the preservation number is CGMCC No. 8504. The Muscovy duck parvovirus screened by the invention has the characteristics of low toxicity and high immunogenicity; dead embryo and embryo liquid can be obtained by vaccinating the Muscovy duck parvovirus to a Muscovy duck embryo; virus liquid can be collected after grinding and freeze thawing; a vaccine is prepared by ultrafiltration concentration, formalin inactivation, the addition of an adjuvant, and mixing and emulsification; the Muscovy duck parvovirus can improve antibody of the Muscovy duck, ensure parent source antibody level of an offspring, and prevent green duck parvovirus infection caused by Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

Description

technical field [0001] The invention belongs to the technical field of poultry pathogenic microorganism screening, and in particular relates to a Muscovy duck parvovirus and its application in preparing vaccines and antibodies. Background technique [0002] Muscovy duck parvovirus disease is an acute or subacute infectious disease that affects young muscovy ducks. It is an infectious disease mainly characterized by panting, diarrhea, pancreatic necrosis and hemorrhage; it is highly contagious, and its morbidity and mortality are high. Very high, often causing serious economic losses. The disease mainly occurs in young muscovy ducks of 1 to 3 weeks of age, especially young muscovy ducks of about 10 days of age. The disease first appeared in Fujian, China, and the Brittany region of western France in the mid-to-late 1980s, and it was recognized as an independent disease different from gosling plague in the early 1990s. If the dead muscovy ducks are not handled properly, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C07K16/08A61K39/23A61P31/20C12R1/93
Inventor 宫晓邹敏朱艳梅徐保娟李陆梅郭伟伟
Owner YEBIO BIOENG OF QINGDAO
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