High-efficiency chicken infectious bronchitis inactivated vaccine and preparation method thereof

Abstract

The invention discloses a high-efficiency chicken infectious bronchitis inactivated vaccine and a preparation method thereof. The virus content of every 0.3ml of the inactivated vaccine is not lower than (8.67+ / -0.81)*10<6>EID50 or not lower than (7.48+ / -0.45)*10<7> virus particle number. The preparation method comprises the following steps: inoculating a chicken infectious bronchitis virus to a chick embryo allantoic cavity, collecting the virus solution, concentrating the virus solution, measuring the content of the concentrated virus solution, inactivating by virtue of a formaldehyde solution, adding an adjuvant, emulsifying so as to prepare the inactivated vaccine. Because the high-efficiency chicken infectious bronchitis inactivated vaccine disclosed by the invention contains the concentrated virus solution, the content of antigens is improved, and the toxicity attack protection rate of a chicken infectious bronchitis breathing-type high-toxicity M41 strain is over 80 percent. Therefore, the inactivated vaccine disclosed by the invention can provide immunoprotection on the chicken infectious bronchitis strain, and the inactivated vaccine has good effects of preventing and controlling the chicken infectious bronchitis.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biomedicine, and in particular relates to an efficient inactivated chicken infectious bronchitis vaccine and a preparation method thereof. Background technique [0002] Infectious bronchitis virus (IBV) belongs to the Coronaviridae family and can cause infectious bronchitis (IB) in chickens. It mainly affects the respiratory system, urinary system and digestive system of chickens, showing extensive tissue tropism and high genetic variability. , can lead to different degrees of disease in chickens of different ages, sexes and varieties, and the mortality rate is high. It has been reported that the mortality rate of chicks caused by glandular stomach type IB can reach 95%. In addition, IBV can also damage the kidneys, reproductive tract, intestines, muscles, etc., resulting in a decrease in weight gain and feed return rate of chickens, and a decrease in egg production and egg quality of layin...

AI Technical Summary

Problems solved by technology

However, the immune effect of the existing IB inactivated vaccines is not ideal, and the challenge protection rate to strains of the same serotype rarely exceeds 50%, mainly because the existing inactivated vaccines cannot induce high enough antibodies

[0005] At present, there are also many reports about concentrating the virus liquid after preparation, but these reports only indicate the concentrated volume, and do not measure the content of the concentrated virus liquid, and there is no fixed antigen content standard. Therefore, the inactivated vaccine The immune effect is unpredictable, therefore, it is necessary to develop a high immune effect IB inactivated vaccine with fixed antigen content standards

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