High-efficiency chicken infectious bronchitis inactivated vaccine and preparation method thereof

A technology of inactivated vaccine and chicken infectivity, which is applied in the field of highly efficient inactivated vaccine for chicken infectious bronchitis and its preparation, which can solve the problem of unsatisfactory immune effect, rarely more than 50% protection rate against virus, and inability to induce antibodies, etc. problem, to achieve a good effect of prevention and control

Inactive Publication Date: 2015-03-04
SUN YAT SEN UNIV
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Problems solved by technology

However, the immune effect of the existing IB inactivated vaccines is not ideal, and the challenge protection rate to strains of the same serotype rarely exceeds 50%, mainly because the existing inactivated vaccines cannot induce high enough antibodies
[0005] At present, there are also many reports about concentrating the virus liquid after preparation, bu

Method used

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  • High-efficiency chicken infectious bronchitis inactivated vaccine and preparation method thereof
  • High-efficiency chicken infectious bronchitis inactivated vaccine and preparation method thereof
  • High-efficiency chicken infectious bronchitis inactivated vaccine and preparation method thereof

Examples

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Example Embodiment

[0022] Example 1 Preparation method of highly effective inactivated chicken infectious bronchitis vaccine

[0023] In this embodiment, the method for preparing the highly effective inactivated chicken infectious bronchitis vaccine includes the following steps:

[0024] Step 1: Preparation of viral solution of infectious bronchitis virus H120 strain

[0025] The preparation virus seed is infectious bronchitis virus H120 strain (purchased from Qingdao Yibang Biological Engineering Co., Ltd.), which is stored at -70°C in the laboratory of the School of Life Sciences, Sun Yat-sen University. The IBV H120 strain was diluted 100-fold and inoculated into the allantoic cavity of 10-day-old SPF chicken embryos, 0.2ml per embryo. After inoculation, the pinholes were sealed with wax and incubated at 37°C. The dead chicken embryos within 24 hours were discarded. The allantoic fluid is harvested 36h-48h after the inoculation, which is the H120 strain of infectious bronchitis virus.

[0026] Ste...

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Abstract

The invention discloses a high-efficiency chicken infectious bronchitis inactivated vaccine and a preparation method thereof. The virus content of every 0.3ml of the inactivated vaccine is not lower than (8.67+/-0.81)*10<6>EID50 or not lower than (7.48+/-0.45)*10<7> virus particle number. The preparation method comprises the following steps: inoculating a chicken infectious bronchitis virus to a chick embryo allantoic cavity, collecting the virus solution, concentrating the virus solution, measuring the content of the concentrated virus solution, inactivating by virtue of a formaldehyde solution, adding an adjuvant, emulsifying so as to prepare the inactivated vaccine. Because the high-efficiency chicken infectious bronchitis inactivated vaccine disclosed by the invention contains the concentrated virus solution, the content of antigens is improved, and the toxicity attack protection rate of a chicken infectious bronchitis breathing-type high-toxicity M41 strain is over 80 percent. Therefore, the inactivated vaccine disclosed by the invention can provide immunoprotection on the chicken infectious bronchitis strain, and the inactivated vaccine has good effects of preventing and controlling the chicken infectious bronchitis.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biomedicine, and in particular relates to an efficient inactivated chicken infectious bronchitis vaccine and a preparation method thereof. Background technique [0002] Infectious bronchitis virus (IBV) belongs to the Coronaviridae family and can cause infectious bronchitis (IB) in chickens. It mainly affects the respiratory system, urinary system and digestive system of chickens, showing extensive tissue tropism and high genetic variability. , can lead to different degrees of disease in chickens of different ages, sexes and varieties, and the mortality rate is high. It has been reported that the mortality rate of chicks caused by glandular stomach type IB can reach 95%. In addition, IBV can also damage the kidneys, reproductive tract, intestines, muscles, etc., resulting in a decrease in weight gain and feed return rate of chickens, and a decrease in egg production and egg quality of layin...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61P31/20
Inventor 曹永长尹丽娟薛春宜
Owner SUN YAT SEN UNIV
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