Method for preparing anti-dog parvovirus egg yolk antibody biological preparation
A technology of canine parvovirus and biological preparations, applied in antiviral agents, antiviral immunoglobulins, antibodies, etc., can solve the problems of high cost, large loss of IgY activity, cumbersome preparation process, etc., and achieve low cost, quick effect, The effect of preventing puppies from parvovirus infection
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Embodiment 1
[0015] Canine parvovirus field virus collection: take the intestines and contents of dead dogs diagnosed as parvovirus infection, add normal saline to grind at a weight ratio of 1:1, centrifuge to remove sediment, and supernatant 20000rpm high-speed centrifugation for 60 minutes to remove tiny particles.
[0016] Initial virus extraction: add chloroform to 10% of the total supernatant, shake vigorously, and centrifuge to remove fat-soluble substances.
[0017] Virus adsorption: 10% of the total supernatant was added to washed porcine red blood cells hydroformylated with 2.5% glutaraldehyde by volume, shaken well and placed at 4°C for 2 hours, and centrifuged to precipitate porcine red blood cells.
[0018] Virus release: use 4% hypertonic saline to make the precipitated porcine red blood cells into a suspension with a volume ratio of 10%, centrifuge at 37° C. for 2 hours to remove the porcine red blood cells.
[0019] Determination of HA titer: the virus titer in the released ...
Embodiment 2
[0028] Canine parvovirus field virus collection, virus initial extraction, virus adsorption and release test purification, HA value detection, and vaccine preparation are the same as in Example 1. For the first immunization, the immunization dose is 0.5ml per head. The second immunization was carried out at an interval of 10 days, and the immunization dose was 1 ml. After 14 days of immunization, the titer of egg yolk was measured by hemagglutination inhibition test. When the titer was lower than 1:128, the third immunization was carried out, and the immune dose was 1ml. After 14 days, the immunized eggs were collected, and the anti-parvovirus egg yolk antibody The preparation method of the biological preparation is the same as in Example 1.
[0029] Preparation of vaccine, immunization, IgY extraction and purification by the above method, the yield of IgY is over 95%, and the activity is not affected in any way. More than 26 mg of IgY can be obtained after extraction from 5 ...
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