The invention discloses a bioengineering lacquer putty as well as preparation method and use thereof. The bioengineering lacquer putty comprises: talcum powder, heavy calcium carbonate, emiaste, hydroxypropylmethylcellulose, adhesive, calcium stearate, tourmaline powder and bergmeal. the bioengineering lacquer putty of the invention has the sterilizing and deodorizing function, can automatically remove the harmful gas such as methyl aldehyde and the like, has no pollution, and ensure the safety of the human body and the animals and plants. The bioengineering lacquer putty of the invention has higher production amount of the negative ion and far infrared emissing ability, can prevent the electromagnetic radiation, can rapidly absorb and remove the abnormal flavour generated by the paint, the colloid and the like, absorb the smoke flavor for a long time, and has definite treating efficacy for the limbs ice-cold disease, shoulder pain, lumbago, physical pain, neuralgia and the like.