37 results about "Hemagglutination Inhibition Tests" patented technology

The invention discloses a GeXP rapid detection kit for identifying eight types of porcine viral diseases and a primer group thereof. Nine pairs of specific primers and a pair of universal primers are designed by the inventor based on research of a GeXP system, accordingly, a GeXP rapid detection method for identifying the eight types of porcine viral diseases is established, and the corresponding detection kit is prepared. The GeXP rapid detection method and the detection kit have the advantages of good specificity, high sensitivity, convenience, high efficiency and the like and can be used for simultaneously detecting and identifying eight types of pathogens. Compared with identification results of conventional experiment methods such as virus isolation and hemagglutination inhibition test, the coincidence rate of the GeXP rapid detection method reaches 100%. The GeXP rapid detection method and the detection kit can be used for effectively solving amplification preference in a multiplex-PCR (polymerase chain reaction) process, provide simple, convenient and high-throughput detection means for detection of main porcine common infectious diseases and pathogens thereof, meet practical requirements and have a broad application prospect.

The invention discloses a preparing method of anti-dog parvoviral vitelline antibody biological preparation, which comprises the following steps: collecting disease material of dead dog with parvoviral infection; centrifuging; removing depositing slag; centrifuging the supernatant fluid; adding into chloroform; vibrating severely; centrifuging; getting the supernatant fluid; aldehyde-washing pig red cell; adding the supernatant into the pig red cell; vibrating evenly; stewing; centrifuging; getting depositing liquid; adding into salt water; stewing; centrifuging; getting virus liberating liquid; testing HA potency with liberating liquid; reaching 1: 4096 effective; deactivating the virus liberating liquid with methyl aldehyde; adding into oil emulsion dog parvoviral vaccine prepared by brown algae polysaccharide; passing the vaccine to hen after delivery; testing HI potency of immune ovum with hemagglutination inhibition test; harvesting vitelline after reaching standard; diluting with sterile distilled water; proceeding acid treatment with lactic acid liquid; removing mixed protein; degreasing with freeze-thaw; salting-out suction liquid; adjusting IgY density; loading; putting into bathing box; treating; getting the oral biological preparation.

The invention discloses a full-automatic Newcastle disease detector and relates to the technical field of animal medical equipment. The full-automatic Newcastle disease detector comprises a red blood cell supply unit, a virus liquid supply unit, a reagent supply unit, a reaction plate production unit, a vision interpretation unit, an interpretation region motion unit, a suction head supply unit, a sample feeding region motion unit and a single-shaft double-sliding block motion unit, wherein all the units are mounted on a base. According to the full-automatic Newcastle disease detector, Newcastle disease blood coagulation test and Newcastle disease blood coagulation inhibition test can be simultaneously carried out on Newcastle disease virus samples. The full-automatic Newcastle disease detector is simple and compact in structure and is suitable for batch, rapid and effective tests.

The invention relates to the technical field of livestock vaccine, and in particular relates to a method for detecting a hemagglutination inhibition antibody of chicken infectious bronchitis. The method comprises the following steps: treating serum to be detected, namely, by taking kaoline suspension as a serum treatment liquid for treating serum to be detected, adding the supernate into chicken erythrocyte blood corpuscle mud so as to obtain serum which is diluted in a ratio of 1:4 and is to be detected; performing hemagglutination inhibition test, namely, adding 25mu l of the serum which is diluted in a ratio of 1:4 and is to be detected into 25mu l of antigen with 4HA units, and performing hemagglutination judgment. By adopting the negative serum treated by using a method for removing non-specific reaction of the serum, the infectious bronchitis hemagglutination inhibition evaluation titer is less than 1:4, and by adopting the infectious bronchitis positive serum treated by using the method, the infectious bronchitis hemagglutination inhibition evaluation titer is reduced by 0.5-1 titer, the non-specificity reaction is removed, and the accuracy of the infectious bronchitis hemagglutination inhibition experiment result is improved.

The present invention discloses a rapid antigen detection method for inactivated oil emulsion vaccine against avian influenza finish products, and the method comprises the following steps of: 1) mixing uniformly isopropyl myristate and the inactivated oil emulsion vaccine against avian influenza by thoroughly shaking, centrifuging, and separating a water phase layer; 2) performing HA titer detection to the water phase of the vaccine obtained in the step 1); 3) according to the detection result of the HA titer detection of the water phase of the vaccine in step 2), taking the water phase of the inactivated oil emulsion vaccine against avian influenza of step 1) to prepare a 4HAU vaccine antigen diluent; and 4) performing hemagglutination inhibition tests by using the 4HAU vaccine antigen diluent prepared in step 3), wherein a HI titer is expressed as the highest dilution serum that completely inhibits the 4HAU antigen. The method of the invention does not destroy the hemagglutination titer and antigenicity of vaccine antigens, can quickly and accurately determine the HI titer of the water phase of the inactivated oil emulsion vaccine against avian influenza finish products and analyze differences in antigenicity, and reagents in use are safety and non-toxic for human and environment, and are cheap and readily available.

The invention discloses a polymerase chain reaction (PCR) primer pair for identifying an H9 subtype avian influenza virus (AIV) and application thereof. The PCR primer pair disclosed by the invention is composed of two single chain deoxyribonucleic acids (DNAs), wherein the two single chain DNAs are single chain DNAs shown in SEQ ID NO:1 and SEQ ID NO:2 in a sequence table. The HA gene of the H9 subtype AIV in a sample can be subjected to specific amplification, and the length of a target segment is 425bp. The method is free of cross reaction on H3, H4, H5 and other subtype AIVs, and a newcastle disease virus, avian infectious bronchitis, an infectious bursal disease virus, an infectious laryngotracheitis virus and the like; the minimum detectable quantity of virus allantoic fluid is 1*10<4.25>EID50/100mu L. Compared with the conventional methods such as a hemagglutination inhibition test of the virus and the like, the accordance rate of the identification result is 100%. A rapid, specific and sensitive detection means is provided for identification of the H9 subtype AIV. The PCR primer pair can be applied to rapid diagnosis of a disease caused by the H9 subtype AIV, and has a good application prospect in the aspects of clinical diagnosis and epidemiological investigation.

The invention relates to QX-type infectious bronchitis virus (IBV) hemagglutination inhibition test antigens, a preparation method thereof and application. A preparation method for an HI antigen comprises the following steps of inoculating 10<4>-10<7> EID<50> of QX-type IBV strains to chicken embryos, carrying out incubation for virus multiplication at a temperature of 37 degrees centigrade, after36-48h, taking out the chicken embryos, carrying out cooling for 6-16h at the temperature of 2-8 degrees centigrade, collecting chicken embryo allantoic liquid, uniformly mixing concentrated chickenembryo allantoic liquid with I-type phosphatase C, carrying out shaker action for 2-2.5h at the temperature of 37 degrees centigrade, and then carrying out placement for 21-35d at the temperature of 2-8 degrees centigrade to obtain antigens. The HI antigens and the detection method provided by the invention are characterized by high specificity, high stability, high sensitivity and high efficiency. Rapid detection can be carried out on clinical serum samples within 2h. The HI antigens and the detection method can be widely applied to quarantine and diagnosis of IB and detection of immunized antibodies. The HI method for detecting QX-type infectious bronchitis becomes feasible. A practical and scientific method for prevention of the IB is provided. The prevention and control over the disease in poultry production is guided well.

The invention discloses a detection method for an avian influenza hemagglutination inhibition test. The detection method comprises the following steps: S1, sequentially numbering a V-shaped micro-reaction plate from 1 to 12, sequentially adding 50 [mu]L of 4 units of an avian influenza virus antigen solution into each of the holes from 1 to 11; S2, respectively adding 50 [mu]L of detected serum into the first hole and the twelfth hole, mixing evenly, then absorbing 50 [mu]L to the second hole from the first hole, sequentially diluting to the tenth hole at multiple proportions, abandoning 50 [mu]L in the tenth hole; S3, sequentially adding 50 [mu]L of 1% chicken erythrocyte suspension into each hole from the twelfth hole to the first hole; S4, oscillating the V-shaped micro-reaction plate on a microoscillator, allowing standing still, incubating, and judging the agglutination condition of each hole when erythrocyte in the twelfth hole forms a button shape and sinks to the bottom of thehole. The detection method is efficient and fast, is high in accuracy, and is suitable for monitoring large chicken flocks by an enterprise.

The invention relates to a hemagglutination inhibition test antigen for a duck tembusu virus disease and a preparation method thereof. A duck hemorrhagic ovarian inflammation virus HB strain is preserved at the typical culture preservation centre in China with the preservation number being CCTCC V201122; the strain comprises a specific gene sequene, and the GenBank accession number of the strain is JF523187. The strain is inoculated againt a neonatal rat proliferating virus; the hemagglutination inhibition test antigen for the duck tembusu virus disease is prepared through the technologies of hemagglutination inhibition nonspecific material (lipoprotein) removal, inactivation, addition of a protective agent and the like. The invention further discloses the preparation method of the hemagglutination inhibition test antigen by a duck tembusu virus.

The invention belongs to the technical field of microbiological detection, and discloses an influenza hemagglutination inhibition test detection method which comprises the following steps: pretreatingstandard reference antiserum to remove non-specific inhibin and non-specific lectin in the serum; preparing an erythrocyte suspension; determining the hemagglutination titer of the influenza virus strain; preparing four hemagglutination unit antigens for an erythrocyte agglutination inhibition test; rechecking and titrating four hemagglutination unit antigens; and performing influenza virus identification and antigen analysis by using an HAI method to obtain the serum titer of the detected serum. According to the detection method, chicken erythrocyte, turkey erythrocyte and guinea pig erythrocyte are used as raw materials to prepare erythrocyte suspension, animal erythrocyte is used for replacing human erythrocyte, and the difficulty that an experiment cannot be conducted due to lack of erythrocyte is solved.

The invention provides an automatic interpretation tool for a hemagglutination and hemagglutination inhibition test result. The tool is a device for automatically generating the result. An interpretation method for the tool comprises the following steps: adjusting a hemagglutination plate support to make erythrocytes form a teardrop shape; carrying out zoning photographing on hemagglutination plate sample holes in a fixed object distance, calculating the teardrop length of the erythrocytes, and finding out a target hole; and processing obtained results, storing original images and interpretation results in a database, carrying out classified statistics according to the test results of a sample and test items, gathering the sample, and outputting the results by clicking a menu. The hemagglutination and hemagglutination inhibition test interpretation device and the statistical method thereof allow 96 samples to be simultaneously interpreted in 4 seconds, so the interpretation speed is much faster than that of manual operation, and the time and the labor are saved. Manual analysis and discrimination are not needed by using the tool, so visual fatigue and misjudgment are avoided, and individual differences are avoided; and the possibility of personnel in contact with pathogens is reduced, and the tool can be connected with a sample information management system to realize automaticmanagement.

The invention relates to a preparation method of a VIb subtype pigeon Newcastle disease positive serum standard substance. The HI titer of the positive serum standard substance prepared by the method is not lower than 1:128, the neutralizing titer is not lower than 1:1600, the serum can completely neutralize the VIb subtype pigeon Newcastle disease virus, the positive serum standard substance is used for detecting the specificity of a positive control standard substance and a vaccine seed virus in a hemagglutination inhibition test and an exogenous virus, the accuracy of an antibody detection result can be ensured in the hemagglutination inhibition test, the antibody level of the immunized pigeons is fully reflected, and a guarantee is provided for accurate monitoring of VIb subtype Newcastle disease epidemic strains in pigeons, immunogenicity and specificity detection of pigeon Newcastle disease seed viruses, exogenous virus detection and vaccine immune efficacy evaluation. The invention fills the blank that no standard positive serum exists in the development of VIb subtype pigeon Newcastle disease vaccines and epidemiological investigation. The preparation method of the positive serum comprises the following steps: preparation of a pigeon Newcastle disease LY/2020/Pi strain inactivated vaccine, animal screening, animal immunization, and preparation, purification and inspection of the positive serum.

The invention provides a VP2 gene and NP gene recombinant adenovirus and application thereof. The preparation method comprises the following steps: respectively amplifying VP2 genes and NP genes fromSD strains of IBDV (Infectious Bursal Disease Virus) and AIV (Avian Influenza Virus), cloning the obtained target genes into shuttle vectors pDC315-MCS-EGFP, performing co-transfection on a recombinant adenovirus shuttle plasmid pDC315-VP2-NP-EGFP, an adenovirus macroskeleton pBHGlox(delta)E1 and 3Cre in HEK293 cells by utilizing a lipofection transfection method, recombining and packaging, thereby obtaining the recombinant adenovirus pBH-VP2-NP-EGFP containing the VP2 genes and NP genes. The recombinant adenovirus is capable of stimulating the body to produce an antibody, the IBDV antibody content determined by an agar diffusion test reaches an effective antibody level, and the AIV antibody content determined by a hemagglutination inhibition test method is obviously superior to that in acontrol group pBH-EGFP; and the challenge test results show that the protection rate reaches 90%.

The invention relates to an influenza virus antigen dissimilarity calculation method and system. The method comprises the following steps: acquiring a hemagglutination inhibition titer set obtained by a known hemagglutination inhibition test, wherein the hemagglutination inhibition titer set comprises known hemagglutination inhibition titers; calculating the antigen distance between strains according to the hemagglutination inhibition titer to obtain an antigen distance set; establishing an antigen dissimilar network according to the antigen distance set; mapping nodes in the antigen dissimilar network into low-dimensional dense vectors based on a network embedding technology; and quantitatively calculating the antigen diversity between every two strains according to the low-dimensional dense vector. According to the method and the system, the antigen dissimilarity is modeled based on the network structure, the nonlinear feature learning capability of deep learning on the network structure is exerted, the antigen dissimilarity between every two strains can be quantitatively calculated, the robustness of antigen dissimilarity calculation is improved, and further, antigenicity analysis except for influenza antigen specificity can be supported, and good expandability and practicability are achieved.

The invention relates to the technical field of poultry antibody detection reagents, particularly to a mycoplasma gallisepticum antibody detection reagent, a preparation method and application thereof. According to the invention, a mycoplasma gallisepticum culture solution is subjected to a series of treatment on, inactivating is performed, the inactivated antigen bacteria solution and a freeze-drying protective agent are uniformly mixed, and freeze drying is performed to obtain the mycoplasma gallisepticum antibody detection reagent; the reagent can be directly used for a hemagglutination inhibition test; a plate agglutination test can be carried out by adding any one coloring agent of methyl violet, amber red and crystal violet into the reagent containing 4-8HA unit of antigen; the hemagglutination value of the detection reagent reaches 26; and the detection reagent not only improves the hemagglutination titer of the mycoplasma gallisepticum antigen, but also has two purposes, is stable, sensitive, strong in specificity, long in storage life, simple to operate, convenient and fast, does not need special equipment and instruments, and can be used for efficacy test, epidemiologicalinvestigation, clinical sample detection and the like of mycoplasma gallisepticum vaccines.

The invention discloses a full-automatic hemagglutination and hemagglutination inhibition test workstation which comprises a storage bottom box, the top of the storage bottom box is fixedly connected with a bottom plate, the top of the storage bottom box is fixedly connected with an upper shell, one side of the upper shell is connected with a door cover through a hinge, one side of the upper shell is provided with a cover opening mechanism, and the cover opening mechanism is connected with the door cover. The hemagglutination inhibition experiment sample detection device has the beneficial effects that the U-shaped fixing plates are additionally arranged, the inner sides of the two U-shaped fixing plates are mounted through different mounting blocks, the bottom plate can accommodate 24 plate positions, the efficiency of detecting a hemagglutination inhibition experiment sample is improved, and the detection accuracy is improved. The time and the cost of manual operation are greatly reduced; by adding the X-axis linear module, the Y-axis linear module and the Z-axis linear module, multi-position adjusting and moving treatment of the fixed box and the pipetting cylinder is realized, and the pipetting efficiency is improved.