37 results about "Hemagglutination Inhibition Tests" patented technology

The invention discloses a polymerase chain reaction (PCR) primer pair for identifying an H9 subtype avian influenza virus (AIV) and application thereof. The PCR primer pair disclosed by the invention is composed of two single chain deoxyribonucleic acids (DNAs), wherein the two single chain DNAs are single chain DNAs shown in SEQ ID NO:1 and SEQ ID NO:2 in a sequence table. The HA gene of the H9 subtype AIV in a sample can be subjected to specific amplification, and the length of a target segment is 425bp. The method is free of cross reaction on H3, H4, H5 and other subtype AIVs, and a newcastle disease virus, avian infectious bronchitis, an infectious bursal disease virus, an infectious laryngotracheitis virus and the like; the minimum detectable quantity of virus allantoic fluid is 1*10<4.25>EID50 / 100mu L. Compared with the conventional methods such as a hemagglutination inhibition test of the virus and the like, the accordance rate of the identification result is 100%. A rapid, specific and sensitive detection means is provided for identification of the H9 subtype AIV. The PCR primer pair can be applied to rapid diagnosis of a disease caused by the H9 subtype AIV, and has a good application prospect in the aspects of clinical diagnosis and epidemiological investigation.

The invention provides a VP2 gene and NP gene recombinant adenovirus and its application. The VP2 gene and NP gene are respectively amplified from the SD strains of IBDV and AIV, and the obtained target gene is cloned into the shuttle vector pDC 315 In ‑MCS‑EGFP, the recombinant adenoviral shuttle plasmid pDC was transfected by lipofection 315 ‑VP2‑NP‑EGFP and adenovirus backbone pBHGlox(delta)E1,3Cre were co-transfected into HEK293 cells, recombined and packaged to obtain recombinant adenovirus pBH‑VP2‑NP‑EGFP containing VP2 gene and NP gene. The recombinant adenovirus can stimulate the body to produce antibodies, and the IBDV antibody content measured by the agar expansion method reached an effective antibody level, and the AIV antibody content measured by the hemagglutination inhibition test method was significantly better than that of the control group pBH‑EGFP; the results of the challenge test showed that the protection rate up to 90%.

The invention discloses a full-automatic Newcastle disease detector and relates to the technical field of animal medical equipment. The full-automatic Newcastle disease detector comprises a red blood cell supply unit, a virus liquid supply unit, a reagent supply unit, a reaction plate production unit, a vision interpretation unit, an interpretation region motion unit, a suction head supply unit, a sample feeding region motion unit and a single-shaft double-sliding block motion unit, wherein all the units are mounted on a base. According to the full-automatic Newcastle disease detector, Newcastle disease blood coagulation test and Newcastle disease blood coagulation inhibition test can be simultaneously carried out on Newcastle disease virus samples. The full-automatic Newcastle disease detector is simple and compact in structure and is suitable for batch, rapid and effective tests.

The present invention discloses a rapid antigen detection method for inactivated oil emulsion vaccine against avian influenza finish products, and the method comprises the following steps of: 1) mixing uniformly isopropyl myristate and the inactivated oil emulsion vaccine against avian influenza by thoroughly shaking, centrifuging, and separating a water phase layer; 2) performing HA titer detection to the water phase of the vaccine obtained in the step 1); 3) according to the detection result of the HA titer detection of the water phase of the vaccine in step 2), taking the water phase of the inactivated oil emulsion vaccine against avian influenza of step 1) to prepare a 4HAU vaccine antigen diluent; and 4) performing hemagglutination inhibition tests by using the 4HAU vaccine antigen diluent prepared in step 3), wherein a HI titer is expressed as the highest dilution serum that completely inhibits the 4HAU antigen. The method of the invention does not destroy the hemagglutination titer and antigenicity of vaccine antigens, can quickly and accurately determine the HI titer of the water phase of the inactivated oil emulsion vaccine against avian influenza finish products and analyze differences in antigenicity, and reagents in use are safety and non-toxic for human and environment, and are cheap and readily available.