VP2 gene and NP gene recombinant adenovirus and application thereof

A gene recombination, adenovirus technology, applied in the field of genetic engineering, can solve the problem of lack of recombinant virus construction method and so on

Active Publication Date: 2018-05-22
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides VP2 gene and NP gene recombinant adenovirus and its application, so as to solve the technical problem of lacking a method for constructing such recombinant virus in the prior art

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  • VP2 gene and NP gene recombinant adenovirus and application thereof
  • VP2 gene and NP gene recombinant adenovirus and application thereof
  • VP2 gene and NP gene recombinant adenovirus and application thereof

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Embodiment Construction

[0023] Specific embodiments of the present invention will be described in detail below. In order to avoid too many unnecessary details, well-known structures or functions will not be described in detail in the following embodiments.

[0024] Approximate language used in the following examples is for quantitative representations, indicating that certain variations in quantities are permissible without altering essential function. Accordingly, values ​​modified by language such as "about", "approximately" and the like are not limited to the exact value itself. In some embodiments, "about" means that the corrected value is allowed to vary within the range of plus or minus ten percent (10%), for example, "about 100" means any value between 90 and 110. Furthermore, in the expression "about the first value to the second value", both values ​​of the first value and the second value are corrected approximately at the same time. In some cases, the language of approximation may relate...

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Abstract

The invention provides a VP2 gene and NP gene recombinant adenovirus and application thereof. The preparation method comprises the following steps: respectively amplifying VP2 genes and NP genes fromSD strains of IBDV (Infectious Bursal Disease Virus) and AIV (Avian Influenza Virus), cloning the obtained target genes into shuttle vectors pDC315-MCS-EGFP, performing co-transfection on a recombinant adenovirus shuttle plasmid pDC315-VP2-NP-EGFP, an adenovirus macroskeleton pBHGlox(delta)E1 and 3Cre in HEK293 cells by utilizing a lipofection transfection method, recombining and packaging, thereby obtaining the recombinant adenovirus pBH-VP2-NP-EGFP containing the VP2 genes and NP genes. The recombinant adenovirus is capable of stimulating the body to produce an antibody, the IBDV antibody content determined by an agar diffusion test reaches an effective antibody level, and the AIV antibody content determined by a hemagglutination inhibition test method is obviously superior to that in acontrol group pBH-EGFP; and the challenge test results show that the protection rate reaches 90%.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, and further relates to the construction technology of recombinant virus, in particular to VP2 gene and NP gene recombinant adenovirus and application thereof. Background technique [0002] Infectious bursal disease (IBD) is an acute and highly contact immunosuppressive infectious disease of poultry caused by chicken infectious bursal virus (IBDV), which mainly affects the central immune organs of chickens. Bursa of Fabricius, resulting in immunosuppression, increased susceptibility to other pathogens, and decreased responsiveness to other vaccines. The disease has a high morbidity rate and a short course. The diseased chickens are mainly manifested as diarrhea, trembling, and extreme weakness, causing death in severe cases. It is extremely harmful to the breeding industry, and the infection of young chickens can lead to immunosuppression and induce the immune failure of various vaccin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/85C12N15/34A61K35/761A61P31/14A61P31/16C12R1/93
CPCA61K35/761C07K14/005C12N7/00C12N15/85C12N2710/10021C12N2710/10022C12N2710/10052
Inventor 高柯柯李亚杰郁宏伟付旭彬刘海霞郑文卿唐荣宏李蓬飞
Owner TIANJIN RINGPU BIO TECH
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