A duck Tembusu virus disease immunotherapy preparation and preparation method thereof
A duck tampusu virus and immunotherapy technology, which is applied in the direction of antiviral agents and antibodies, can solve problems such as no research reports on immunotherapy of duck tembusu virus disease, and achieve suitable for large-scale production, good therapeutic effect, The effect of simple equipment
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[0030] Specific embodiment: the preparation method of duck Tembusu virus disease immunotherapy preparation
[0031] The preparation steps of duck Tembusu virus disease immunotherapy preparation are as follows:
[0032] The seed strain used for the antigen of the present invention is Flaviviridae Flavivirus genus Yatambusu virus Guangdong strain (JM), which was sent to the China Center for Type Culture Collection on October 14, 2011 for preservation, and the preservation number is CCTCCV201131.
[0033] 1. Breeding of poisonous species
[0034] Inoculate 11-day-old shelduck duck embryos with the 7th generation allantoic fluid poison of JM strain (code name JMDE7, ELD50 is 10-6.0 / ml) through the allantois, 0.2ml / embryo, culture at 37°C, irradiate embryos twice a day, discard After 24 hours of dead embryos, the dead duck embryos were placed in a 4°C refrigerator, and the dead embryo allantoic fluid (hereinafter referred to as toxic allantoic fluid) was aseptically harvested with...
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