7 results about "Allantois" patented technology

The invention belongs to the technical field of poultry disease prevention and treatment, and discloses application of a rhizoma kaempferiae extract in preparation of a medicine for resisting avian infectious bronchitis virus. The rhizoma kaempferiae extract is prepared by taking rhizoma kaempferiae as a raw material and ethanol as an extraction solvent through extraction, filtration and concentration. Experimental results show that the rhizoma kaempferiae extract has no obvious toxicity to chick embryos within a certain dosage range; after the chicken embryo is infected with the infectious bronchitis virus, the survival rate of the chicken embryo can be improved by treating the chicken embryo with different doses of the rhizoma kaempferiae extract, and the rhizoma kaempferiae extract can reduce the virus antigen expression level and virus copy number in the liver tissue and allantoic fluid of the chicken embryo, so that the chicken embryo has prevention and treatment effects on infectious bronchitis virus infection. The rhizoma kaempferiae extract has the advantages of easily available raw materials, simple process and low cost, and is suitable for popularization and application.

The utility model belongs to the technical field of biological pharmacy, and particularly relates to a chick embryo allantoic fluid collecting device which comprises a syringe needle, a needle stem and a pintle. The anti-blocking filtering piece comprises a sleeve, a housing and a connecting piece, the front end of the needle stem is covered with the housing, the risk that yolk is damaged in the collecting process can be reduced by means of the characteristics that the outer surface of the housing is smooth and free of edges and corners, and the egg yolk collecting device is convenient, rapid and high in yield and provides convenient conditions for follow-up production; a plurality of liquid inlet holes are formed outside the housing, so that liquid inlet paths can be dispersed, and the blocking risk caused by a single channel is reduced; the needle stem is sleeved with the sleeve, stable support is provided for the housing, and the stability of the collecting process is guaranteed; and finally, the connecting piece is arranged to be detachably connected with the pintle, so that assembling and disassembling can be facilitated, and cleaning and replacement are facilitated.

The invention relates to the technical field of biological medicine and vaccine preparation, and discloses a preparation method of a tetravalent influenza virus split vaccine, and the preparation method comprises the following steps: inoculating four influenza viruses into a chick embryo, culturing, harvesting allantoic fluid, and concentrating; centrifugally purifying through a sucrose density zone, and adding a cracking agent for cracking; removing the cracking agent from the cracking solution through chromatographic column chromatography, and then adding an inactivating agent for inactivating and removing the inactivating agent; according to the method disclosed by the invention, a process route of firstly splitting purification and then inactivation in combination with dual purification of centrifugation and chromatography is adopted, so that the problem of impurity cross-linking caused by an inactivator in a traditional process is effectively avoided, and residues of ovalbumin and splitting agents are deeply removed; and the natural conformation of the virus antigen is retained to the greatest extent. The obtained vaccine is extremely low in impurity content, has excellent safety and high immunogenicity, and can effectively expand the immune protection range and enhance the population immune barrier.

The method comprises the following steps: collecting a traditional Chinese medicine containing isoquinoline alkaloid, and finishing the property and flavor tropism and the cold-heat property of the traditional Chinese medicine; preparing liquid medicine with gradient concentration; the method comprises the following steps of: incubating a quail embryo egg, opening a hole to remove an upper shell membrane, dropwise adding PBS (Phosphate Buffer Solution) on the allantoic membrane of the survival quail embryo egg, observing the distribution condition of blood vessels, respectively injecting liquid medicine into the allantoic membrane of the quail embryo egg at a position without blood vessel development, sealing the hole, incubating, and taking all blood vessels connected with the quail embryo and the allantoic membrane of the quail embryo egg as blood vessel samples; the influence difference of the traditional Chinese medicine containing the isoquinoline alkaloids on the angiogenesis area, the total blood vessel number and the capillary vessel number of the quail embryo egg allantoic membrane is tested and evaluated through LSD-t; the method has the advantages that the method is fast, convenient and low in cost, and objective and accurate basis is provided for evaluating the cold-heat property of the traditional Chinese medicine.

The invention relates to the field of prevention and control of poultry viral diseases, in particular to application of herba epimedii extract in preparation of a medicine for preventing and / or treating infectious bronchitis virus infection. The herba epimedii extract is a herba epimedii ethanol extract. The biological safety and antiviral activity of the extract are evaluated by constructing a chick embryo infection model, and the epimedium extract does not generate obvious toxic influence on chick embryos; after infectious bronchitis virus infection, the survival rate of chick embryos can be increased. Furthermore, tissue immunofluorescence detection, western blot analysis and probe method fluorescent quantitative PCR detection results show that the extract can reduce the virus antigen expression level and virus nucleic acid copy number in chick embryo liver tissue and allantoic fluid, and therefore the effect of preventing or treating IBV infection is achieved. The herba epimedii extract is stable in raw material source, simple and convenient in preparation process and relatively low in production cost, and has a good popularization and application prospect.

The application discloses an influenza virus subunit vaccine and a preparation method thereof, and relates to the field of biological medicines. Each dose contains H1N1, H3N2 and Victoria; the influenza virus subunit vaccine is prepared through virus inoculation, proliferation culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, lysis purification, mixing, sterilization filtration and subpackaging. The application significantly enhances the dendritic cell targeted uptake and rapid release of antigens through the design of mannose modified small liposomes and pH responsive channels; in combination with the precise controlled release of the graded lysis micelles, the antibody titer and immunological persistence are obviously improved, the single dose protection period is longer, and the defects of the traditional trivalent vaccine in the insufficient immunological intensity are effectively made up.

The invention belongs to the technical field of basic medicine, and relates to a construction method and application of a chick embryo high-sugar microenvironment model based on microinjection in a chorioallantoic cavity, glucose is injected into the chorioallantoic cavity on the 5th day and the 7th day of egg incubation to construct the chick embryo high-sugar microenvironment model, the maternal effect can be eliminated, and the chick embryo high-sugar microenvironment model is obtained. The method can be used for independently researching the influence of the high-glucose microenvironment on the embryonic development, is low in cost, easy to operate, easy to achieve a relatively high experimental sample size, relatively large in embryos and convenient to directly observe and operate, and can become a powerful tool for researching the influence of the high-glucose microenvironment on the embryonic development and filial generation health.