13 results about "Allantois" patented technology

The invention belongs to the technical field of poultry disease prevention and treatment, and discloses application of a rhizoma kaempferiae extract in preparation of a medicine for resisting avian infectious bronchitis virus. The rhizoma kaempferiae extract is prepared by taking rhizoma kaempferiae as a raw material and ethanol as an extraction solvent through extraction, filtration and concentration. Experimental results show that the rhizoma kaempferiae extract has no obvious toxicity to chick embryos within a certain dosage range; after the chicken embryo is infected with the infectious bronchitis virus, the survival rate of the chicken embryo can be improved by treating the chicken embryo with different doses of the rhizoma kaempferiae extract, and the rhizoma kaempferiae extract can reduce the virus antigen expression level and virus copy number in the liver tissue and allantoic fluid of the chicken embryo, so that the chicken embryo has prevention and treatment effects on infectious bronchitis virus infection. The rhizoma kaempferiae extract has the advantages of easily available raw materials, simple process and low cost, and is suitable for popularization and application.

The utility model relates to the technical field of hatching devices, and discloses a goose egg incubator which comprises an incubator shell, a cavity is formed in the bottom of the incubator shell, a ventilation cavity is formed in the inner cavity of the incubator shell, a fan is fixedly installed at the bottom end of the inner cavity of the ventilation cavity, and an air outlet is formed in the inner cavity of the incubator shell. A cylindrical water absorption sponge is placed at the bottom end of an inner cavity of the water storage tank, a water absorption sponge block is fixedly connected to the top end of the cylindrical water absorption sponge, a partition plate is fixedly connected to the side wall of the inner cavity of the incubator shell, ventilation holes are formed in the side face of the partition plate, and a ventilation opening is formed in the top end of the incubator shell. The constant-temperature incubator has the advantages that the constant-temperature incubator is provided with the water-absorbing sponge block, the fan and the vent holes, accordingly, evaporation of water in eggs can be reduced by the aid of high humidity at the early hatching stages, the water required by embryos can be kept, and the constant-temperature incubator is favorable for forming amniotic fluid and allantoic fluid of the embryos.

The utility model belongs to the technical field of biological pharmacy, and particularly relates to a chick embryo allantoic fluid collecting device which comprises a syringe needle, a needle stem and a pintle. The anti-blocking filtering piece comprises a sleeve, a housing and a connecting piece, the front end of the needle stem is covered with the housing, the risk that yolk is damaged in the collecting process can be reduced by means of the characteristics that the outer surface of the housing is smooth and free of edges and corners, and the egg yolk collecting device is convenient, rapid and high in yield and provides convenient conditions for follow-up production; a plurality of liquid inlet holes are formed outside the housing, so that liquid inlet paths can be dispersed, and the blocking risk caused by a single channel is reduced; the needle stem is sleeved with the sleeve, stable support is provided for the housing, and the stability of the collecting process is guaranteed; and finally, the connecting piece is arranged to be detachably connected with the pintle, so that assembling and disassembling can be facilitated, and cleaning and replacement are facilitated.

The invention relates to the technical field of biological medicine and vaccine preparation, and discloses a preparation method of a tetravalent influenza virus split vaccine, and the preparation method comprises the following steps: inoculating four influenza viruses into a chick embryo, culturing, harvesting allantoic fluid, and concentrating; centrifugally purifying through a sucrose density zone, and adding a cracking agent for cracking; removing the cracking agent from the cracking solution through chromatographic column chromatography, and then adding an inactivating agent for inactivating and removing the inactivating agent; according to the method disclosed by the invention, a process route of firstly splitting purification and then inactivation in combination with dual purification of centrifugation and chromatography is adopted, so that the problem of impurity cross-linking caused by an inactivator in a traditional process is effectively avoided, and residues of ovalbumin and splitting agents are deeply removed; and the natural conformation of the virus antigen is retained to the greatest extent. The obtained vaccine is extremely low in impurity content, has excellent safety and high immunogenicity, and can effectively expand the immune protection range and enhance the population immune barrier.

An automatic vaccine harvesting device comprises a feeding device used for being in butt joint with an upstream device conveying egg tray; the shell cutting device comprises a conveying module and a shell cutting module and is used for cutting off an eggshell on the upper side of the embryo egg on the egg tray; the harvesting device comprises a harvesting module and a detection module and is used for harvesting allantoic fluid in the embryo eggs and detecting whether the allantoic fluid is qualified or not; the crushing equipment comprises a transfer mechanism, a turnover mechanism and a crushing mechanism, the turnover mechanism turns over the egg tray, and the embryo eggs fall into the crushing mechanism on the lower side to be crushed. The chick embryo harvesting machine has the advantages that chick embryo conveying, chick embryo disinfection, chick embryo shell cutting, broken shell discharging, chick embryo screening, allantoic fluid harvesting, waste embryo treatment and other operations are achieved, the harvesting efficiency is high, and the harvesting quality is guaranteed.

The application discloses a natural weak strain of infectious laryngotracheitis virus with good safety and immunogenicity. The strain is named as infectious laryngotracheitis virus ILTV FJ19, and has been preserved in China Center for Type Culture Collection on November 29, 2013, with a preservation number of CCTCC NO: V2023109. Experiments show that the chicken infectious laryngotracheitis virus ILTV FJ19 strain of the application has stable passage ability, can be adapted to allantoic cavity inoculation, has good safety and immunogenicity when inoculating 14-day-old SPF chickens, and can be immunized by eye dropping or oral administration. The virus can be used as a weak live vaccine candidate strain of ILTV.

An isolated infectious bronchitis (IB) virus (IBV) QX1285 strain under accession number DPS RE RSCIC 41 in the IZSLER biobank. A vaccine comprising the isolated infectious bronchitis virus and a carrier. The virus may be inactivated. The virus may alternatively be live and attenuated. The vaccine may be formulated for an avian. The avian may be a chicken. The vaccine may be administrated via drinking water. Also claimed is an isolated infectious bronchitis virus having an S1 protein encoded by SEQ ID NO: 1 or a sequence having at least 96% sequence identity thereto, for use as a vaccine against infectious bronchitis. Also claimed is a method of vaccinating an avian against infectious bronchitis, comprising administering the vaccine. Also claimed is a process for preparing a live infectious bronchitis vaccine comprising passaging the virus to reduce pathogenicity, harvesting the attenuated virus, and processing the virus to produce a vaccine. The virus may be passaged at least 50 times. The virus may be passaged by culturing in allantoic fluid of chicken embryos.

The invention belongs to the technical field of biological medicine, and discloses application of a chick embryo allantoic membrane model in lung adenocarcinoma drug resistance mechanism research. The chick embryo allantoic membrane model is obtained by hatching fertilized white-shell eggs in a bird incubator at 37.5 DEG C and 65% humidity. After cancer cells are transplanted to Y-shaped blood vessels of the chorioallantoic membrane, H2228 cells continuously expressing Venus fluorescent protein are transplanted to the chorioallantoic membrane so as to confirm the implantation condition. Before and after medicine injection, all organs of the cut tumor and embryo are shot under fluorescence, and the fluorescence intensity of all the organs is quantitatively analyzed through a fluorescein filter. According to the invention, a chick embryo allantoic membrane model is used for verifying the cancer inhibition effect of Chb-M'in ALK mutant lung adenocarcinoma cells for the first time, the experiment cost is relatively low, and the experiment period is relatively short.

The invention discloses an inoculating and harvesting all-in-one machine, which comprises a disinfection device for disinfecting the upper surface of an embryo egg; the inoculation equipment comprises an egg jacking module, an inoculation module and a disinfection module, the inoculation module comprises an inoculation needle, and the disinfection module is used for disinfecting the inoculation needle; the harvesting equipment comprises a shell cutting module, a harvesting module and a detection module, the shell cutting module is used for cutting off the upper eggshell of the embryo egg, the detection module is used for detecting whether the embryo egg is qualified or not, the harvesting module comprises a plurality of harvesting heads, and the detection module is used for detecting the quality of the harvested liquid; and the caching equipment is used for caching the egg trays. The device has the beneficial effects that the processes of embryo egg conveying, embryo egg disinfection, egg jacking, punching, virus inoculation, embryo egg shell cutting, embryo egg screening and allantoic fluid harvesting are achieved, the processing efficiency is high, pollution is reduced, and the production quality is improved.

The method comprises the following steps: collecting a traditional Chinese medicine containing isoquinoline alkaloid, and finishing the property and flavor tropism and the cold-heat property of the traditional Chinese medicine; preparing liquid medicine with gradient concentration; the method comprises the following steps of: incubating a quail embryo egg, opening a hole to remove an upper shell membrane, dropwise adding PBS (Phosphate Buffer Solution) on the allantoic membrane of the survival quail embryo egg, observing the distribution condition of blood vessels, respectively injecting liquid medicine into the allantoic membrane of the quail embryo egg at a position without blood vessel development, sealing the hole, incubating, and taking all blood vessels connected with the quail embryo and the allantoic membrane of the quail embryo egg as blood vessel samples; the influence difference of the traditional Chinese medicine containing the isoquinoline alkaloids on the angiogenesis area, the total blood vessel number and the capillary vessel number of the quail embryo egg allantoic membrane is tested and evaluated through LSD-t; the method has the advantages that the method is fast, convenient and low in cost, and objective and accurate basis is provided for evaluating the cold-heat property of the traditional Chinese medicine.

The invention relates to the field of prevention and control of poultry viral diseases, in particular to application of herba epimedii extract in preparation of a medicine for preventing and / or treating infectious bronchitis virus infection. The herba epimedii extract is a herba epimedii ethanol extract. The biological safety and antiviral activity of the extract are evaluated by constructing a chick embryo infection model, and the epimedium extract does not generate obvious toxic influence on chick embryos; after infectious bronchitis virus infection, the survival rate of chick embryos can be increased. Furthermore, tissue immunofluorescence detection, western blot analysis and probe method fluorescent quantitative PCR detection results show that the extract can reduce the virus antigen expression level and virus nucleic acid copy number in chick embryo liver tissue and allantoic fluid, and therefore the effect of preventing or treating IBV infection is achieved. The herba epimedii extract is stable in raw material source, simple and convenient in preparation process and relatively low in production cost, and has a good popularization and application prospect.

The application discloses an influenza virus subunit vaccine and a preparation method thereof, and relates to the field of biological medicines. Each dose contains H1N1, H3N2 and Victoria; the influenza virus subunit vaccine is prepared through virus inoculation, proliferation culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, lysis purification, mixing, sterilization filtration and subpackaging. The application significantly enhances the dendritic cell targeted uptake and rapid release of antigens through the design of mannose modified small liposomes and pH responsive channels; in combination with the precise controlled release of the graded lysis micelles, the antibody titer and immunological persistence are obviously improved, the single dose protection period is longer, and the defects of the traditional trivalent vaccine in the insufficient immunological intensity are effectively made up.

The invention belongs to the technical field of basic medicine, and relates to a construction method and application of a chick embryo high-sugar microenvironment model based on microinjection in a chorioallantoic cavity, glucose is injected into the chorioallantoic cavity on the 5th day and the 7th day of egg incubation to construct the chick embryo high-sugar microenvironment model, the maternal effect can be eliminated, and the chick embryo high-sugar microenvironment model is obtained. The method can be used for independently researching the influence of the high-glucose microenvironment on the embryonic development, is low in cost, easy to operate, easy to achieve a relatively high experimental sample size, relatively large in embryos and convenient to directly observe and operate, and can become a powerful tool for researching the influence of the high-glucose microenvironment on the embryonic development and filial generation health.