A rapid antigen detection method for inactivated oil emulsion vaccine against avian influenza finish products
A technology of avian influenza oil emulsion and inactivated vaccine, which is applied in the field of rapid detection of finished product antigen of avian influenza oil emulsion inactivated vaccine, can solve problems such as chloroform is harmful to the environment, difficult to store safely, and affects the accuracy of HA potency, and achieves Safe and non-toxic to the human body and the environment, reducing time and capital costs, cheap and easy to obtain for the human body and the environment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] 1. Antigen aqueous phase separation of recombinant avian influenza oil emulsion inactivated vaccine (H5N1 subtype, Re-6 strain)
[0023] Step 1) Take 10ml of recombinant avian influenza oil emulsion inactivated vaccine (H5N1 subtype, Re-6 strain), put it into a 50ml centrifuge tube, add 5-15ml of isopropyl myristate, and place it in a vortex shaker for vigorous shaking more than 5 minutes;
[0024] Step 2) Centrifuge at a speed of more than 3500rpm for more than 5 minutes, oil and water can be seen separated;
[0025] Step 3) Separate about 3ml of the aqueous phase layer.
[0026] Two, get the above-mentioned avian influenza oil emulsion inactivated vaccine aqueous phase, carry out HA potency detection to the vaccine aqueous phase according to the hemagglutination test method of the prior art
[0027] 1) Add 0.025 ml of isotonic phosphate buffered saline (PBS buffer) to wells 1 to 12 of the microreaction plate, and change the dropper.
[0028] 2) Pipette 0.025ml of t...
Embodiment 2
[0044] Example 2 Comparison of Antigen Water Phase HA Titer Determination before and after Demulsification of Recombinant Avian Influenza Oil Emulsion Inactivated Vaccine (H5N1 Subtype, Re-6 Strain)
[0045]Step 1) Take the same batch of H5 subtype Re-6 strain recombinant avian influenza vaccine inactivated with the same batch of known HA titers, and wash them with appropriate amounts of pH 7.2 and 0.01mol / L isotonic phosphate buffered saline (PBS buffered saline) respectively. liquid) diluted to HA titers of 9 log2, 8 log2, 7 log2, and 6 log2 respectively, and the numbers were 1A to 4A in turn, and they were respectively inactivated according to the recombinant avian influenza oil emulsion vaccine (H5N1 subtype, Re-6 strain) Four batches of vaccines were prepared according to the formula and process stipulated in the manufacturing and inspection trial regulations, and the numbers were 1#~4#.
[0046] Step 2) Demulsify the 4 batches of vaccines numbered 1# to 4# respectively a...
Embodiment 3
[0048] Example 3 Comparative analysis of antigenicity of finished product of avian influenza oil emulsion inactivated vaccine
[0049] Step 1) Take commercially available H5 subtype Re-6 strain recombinant avian influenza vaccine, H5 subtype Re-5 strain recombinant avian influenza vaccine, H5 subtype Re-1 strain recombinant avian influenza vaccine and H9 type Re-2 strain recombinant avian influenza vaccine Each bottle of influenza vaccine was demulsified according to the method described in Example 1 to obtain vaccine aqueous phases numbered Re-6, Re-5, Re-1, and Re-2 respectively.
[0050] Step 2) According to the method described in Example 1, the HA titers of the vaccine water phases of Re-6, Re-5, Re-1, and Re-2 were determined to be 9 log2, 8 log2, 8 log2, and 9 log2, respectively.
[0051] Step 3) According to the HA titer test results of the vaccine water phase, adopt the preparation method of the 4HAU vaccine antigen dilution solution in Example 1, and respectively tak...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com