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A screening method for hemagglutination and hemagglutination inhibition test

A technology of hemagglutination inhibition test and screening method, which is applied in biological testing, material testing products, etc., can solve the problems of large workload, large amount of antigen, time-consuming and labor-intensive, etc., reducing workload, saving detection reagents, and high detection efficiency. Effect

Active Publication Date: 2019-02-12
王琴
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Problems solved by technology

[0003] The purpose of the present invention is to provide a screening method for hemagglutination and hemagglutination inhibition test, aiming at solving the problem that when the primary veterinary laboratory conducts the hemagglutination inhibition test, the amount of antigen required is large, and more experimenters are required to perform the test operation, and the work Excessive volume, time-consuming and labor-intensive problems

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  • A screening method for hemagglutination and hemagglutination inhibition test
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  • A screening method for hemagglutination and hemagglutination inhibition test

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[0029] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0031] A screening method for hemagglutination and hemagglutination inhibition test, comprising the following steps:

[0032] Step 1: Dilution

[0033] Prepare 1×PBS solution, after the antigen and serum are opened, fully dissolve with 1ml or 2ml PBS solution according to the product specifications shown on the label of each bottle, and set aside;

[0034] The 1×PBS solution is NaCl 8g, NaH 2 PO 4 2H 2 O 1.561g, Na 2 HPO 4 12H 2 O 3.582g, add water to 100...

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Abstract

The invention discloses a screening method for HA (hemagglutination) and HI (hemagglutination inhibition) tests. The screening method comprises steps as follows: dilution of antigen and serum; an HA test; screening of HI tests, wherein in screening of the HI tests, 80 parts of serum samples are detected once through one dilution plate and three reaction plates, positive samples are screened out, and the level of an immune antibody is finally judged. Compared with a conventional method, the method has the advantage that more than a half of antigens can be saved. More samples are detected by fewer antigens, so that the purpose of detecting the level of the antibody is achieved. The detection method is simple, the detection result is accurate and reliable, the detection efficiency is high, workload is greatly reduced, quantity demands for experimental persons are reduced, and the problems that a general laboratory is short of detection funds, detection reagents are limited and the detection number is large are well solved. The screening method is particularly applicable to primary organizations for epidemic prevention and quarantine of rural animals, the detection reagents are saved, and the detection speed is also increased.

Description

technical field [0001] The invention belongs to the technical field of biological immunology, and in particular relates to a screening method for hemagglutination and hemagglutination inhibition test. Background technique [0002] In veterinary immunology experiments, the hemagglutination test (HA) is mainly used to determine the hemagglutination value of the antigen and whether there is a hemagglutination antigen, while the hemagglutination inhibition test (HI) is widely used in veterinary medicine as an antibody monitoring technology. Laboratory monitoring, veterinary clinics, large and medium poultry farms. As a necessary technical means for grassroots animal epidemic prevention and quarantine institutions, laboratory inspection meets the needs of animal epidemic prevention and quarantine work. In practical applications, HA and HI tests can be used for virus isolation, identification, typing, antigen antibody detection, immune identification, etc. Antibody levels, as a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 王琴李成明王婷楠
Owner 王琴
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