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Method for producing working solution for virus hemagglutination inhibition tests and device applied thereby

A technology of hemagglutination inhibition test and inhibition test, which is applied in the field of hemagglutination inhibition test working solution, can solve the problems of unsuitable field application, long reaction time, complicated methods, etc., and achieve the effects of short reaction time, convenient handling and portability

Inactive Publication Date: 2010-01-27
辽宁省动物疫病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] The existing test methods have the following disadvantages: complex methods, long reaction time, more equipment used, and difficulty in blood collection. Therefore, they are only used in small laboratories and are not suitable for on-site applications.

Method used

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  • Method for producing working solution for virus hemagglutination inhibition tests and device applied thereby
  • Method for producing working solution for virus hemagglutination inhibition tests and device applied thereby
  • Method for producing working solution for virus hemagglutination inhibition tests and device applied thereby

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The preparation of hemagglutination inhibition test working solution of the present invention: 200g sodium citrate is added in 1000mL water, after water bath dissolves, then add glucose 500g, sodium chloride (NaCl) 170g, chromium chloride (CrCl) 3 ·6H 2 O) 2g, sodium azide (NaN 3 ) 10g, adjust the pH to 7.0 with NaOH.

[0045] Make a 20-fold dilution when used.

Embodiment 2

[0047] The preparation of hemagglutination inhibition test working solution of the present invention: 200g sodium citrate is added in 1000mL water, after water bath dissolves, then add glucose 500g, sodium chloride (NaCl) 160g, potassium dihydrogen phosphate (KH 2 PO 4 ) 4g, disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 58g, potassium chloride (KCl) 4g, chromium chloride (CrCl 3 ·6H 2 O) 2g, sodium azide (NaN 3 ) 10g, adjust the pH to 7.0 with NaOH.

[0048] Make a 20-fold dilution when used.

Embodiment 3

[0050] Preparation of hemagglutination inhibition test working solution of the present invention: 200g sodium citrate is added in 1000mL water, after water bath dissolves, then add glucose 500g, sodium chloride (NaCl) 170g, potassium dihydrogen phosphate (KH 2 PO 4 ) 13.6g, chromium chloride (CrCl 3 ·6H 2 O) 2g, sodium azide (NaN 3 ) 10g, adjust the pH to 7.0 with NaOH.

[0051] Make a 20-fold dilution when used.

[0052]An application device for a virus hemagglutination inhibition test working fluid, comprising a box body 1, the box body 1 is composed of a box cover 13, a box bottom 3 with a handle 2, and a plate glass 18 is arranged in the baffle plate 16 of the box cover 13; The bottom of the box 3 is provided with a detection tool box 19, and the detection tool box 19 is provided with a partition 8, and the partition 8 is provided with a medicine bottle 5, a micro pipette 11, a blood coagulation reaction plate 17, a centrifuge tube 15, a centrifuge tube rack 14, Sucti...

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Abstract

The invention discloses a method for producing working solution for virus hemagglutination inhibition tests and a device applied thereby, and belongs to the veterinary speciality in the field of agriculture. The invention provides a method for producing working solution used on the scene for virus hemagglutination inhibition tests and a device applied thereby. The method comprises that: 100 to 300g of sodium citrate, 400g to 600g of glucose, 150 to 170g of sodium chloride, 1 to 5g of chromic chloride and 10 to 20g of sodium azide are added to 1,000ml of water, and pH is adjusted to 7.0 by NaOH. The device comprises a tank body consisting of a tank cover and a tank bottom with a handle, and has the structural key points that: plate glass is arranged in a baffle of the tank cover; a testing tool box is arranged on the tank bottom, and is provided with compartments inside; and the insides of the compartments are provided with medicament bottles, micro pipette tips, hemagglutination reactive plates, centrifugal tubes, a centrifuge tube shelf, suction tubes, plates and a pullorum disease detector with a bulb.

Description

Technical field: [0001] The invention belongs to the field of veterinary medicine in the field of agriculture, and in particular relates to a production method of a hemagglutination inhibition test working fluid for monitoring animal virus antibodies containing hemagglutinin (HA) and an improvement of an application device thereof. Background technique: [0002] Hemagglutinin-containing animal viruses such as Newcastle disease, avian influenza, and chicken egg drop syndrome are all viral infectious diseases that seriously endanger the development of the breeding industry. The optimal timing of immunization is determined through immune monitoring, and timely immunization is the best way to control the occurrence of these major infectious diseases. The hemagglutination inhibition (HI) test is currently the gold standard recommended by the International Organization for Animal Health (OIE) for antibody detection such as Newcastle disease and avian influenza, and is also the ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
Inventor 姜新崔玉君邵传明张鹏顾贵波崔漓波葛宝伟李微荞
Owner 辽宁省动物疫病预防控制中心
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