Reagent strip for jointly detecting coxsackievirus A6 type and A10 type IgM antibody and preparation method of reagent strip

A coxsackie virus and antibody detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of difficult development in grassroots laboratories, high technical requirements, and difficult popularization, etc., and achieve strong adsorption capacity, Solve the difficulty of blood collection, good effect

Inactive Publication Date: 2019-06-21
山东康华生物医疗科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although polymerase chain reaction (PCR) detection is fast, specific and sensitive, its high sensitivity also makes the PCR method relatively demanding on the experimental environment and equipment, and there are problems such as high technical requirements, complicated operation, and difficulty in popularization , it is difficult for general grassroots laboratories to carry out, and the price is relatively high

Method used

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  • Reagent strip for jointly detecting coxsackievirus A6 type and A10 type IgM antibody and preparation method of reagent strip
  • Reagent strip for jointly detecting coxsackievirus A6 type and A10 type IgM antibody and preparation method of reagent strip
  • Reagent strip for jointly detecting coxsackievirus A6 type and A10 type IgM antibody and preparation method of reagent strip

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment one: as attached figure 1 - attached figure 2 As shown, a test strip for detection of Coxsackie virus A6 and A10 type IgM antibodies, the test strip 1 includes a base layer 11, and the base layer 11 is successively provided with a sample cushion layer 12, a latex layer 13, and nitrocellulose The plain film 14 and the water-absorbing layer 15; and the top of the latex layer 13 is covered with a white sticker 17; the top of the water-absorbing layer 15 is covered with a special label strip 16.

[0058] The latex layer 13 is a polyester film with a mouse anti-human IgM monoclonal antibody on the solid phase.

[0059] The sample cushion layer 12 includes a first sample cushion layer 121 fixed on the base layer 11, and the first sample cushion layer 121 is a blood filter membrane; it is fixed on the base layer 11 and covers the first sample cushion layer 121. A second sample cushion layer 122 above a sample cushion layer 121; the area of ​​the second sample cus...

Embodiment 2

[0062] A preparation method of a test strip for Coxsackievirus A6, A10 type IgM antibody detection,

[0063] The preparation method comprises the following steps:

[0064] Step 1: Paste the nitrocellulose membrane 14 on the middle position of the base layer 11;

[0065] Step 2: Paste the water-absorbing layer 15 on one end of the fixed nitrocellulose membrane 14 on the base layer 11, and the water-absorbing layer 15 covers the edge of the nitrocellulose membrane 14 by about 1.0 mm;

[0066] Step 3: The water-absorbing layer 15 is covered with a special label strip 16;

[0067] Step 4: paste the latex layer 13 on the other end of the nitrocellulose membrane 14 on the base layer 11, and the latex layer 13 covers the nitrocellulose membrane 14 by about 1.0 mm;

[0068] Step 5: Paste the first sample cushion layer 121 at the position close to the end surface of the base layer 11, with a width of about 7.0 mm;

[0069] Step 6: paste the second sample cushion layer 122 on the fre...

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Abstract

The invention provides a reagent strip for jointly detecting a coxsackievirus A6 type and A10 type IgM antibody and a preparation method of the reagent strip. A first sample cushion layer is a blood filtering membrane; the surface of the blood filtering membrane is uneven; the blood filtering membrane retains red blood cells by means of physical adsorption; and a sample passes through a second sample cushion layer, a rabbit anti-human red blood cell polyclonal antibody is added into the pretreatment liquid of the second sample cushion layer on the basis of a PBS system, and can be specificallybound to the human red blood cells, and therefore, the red blood cells in the whole blood sample can be further eliminated, and the detection of the whole blood sample can be realized.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis. [0002] Specifically, it relates to a test strip for detecting Coxsackievirus A6 and A10 type IgM antibodies and a preparation method thereof. Background technique [0003] Hand, foot and mouth disease (HFMD) is a common acute infectious disease among infants and young children. For children under 5 years old. Severe complications of central nervous system symptoms such as aseptic meningitis, encephalitis, and acute flaccid paralysis may occur in a small number of patients, and severe cases may lead to death. Since the outbreak of HFMD in my country in 2008, HFMD has become one of the serious public health problems in my country. As of April 2018, 16.448 million cases of HFMD and 3545 infant deaths have been reported nationwide. [0004] In recent years, the main pathogens causing HFMD in mainland China are enterovirus 71 (EV71) and coxsackievirus A16 (CoxA16). However, since 200...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/531
Inventor 杨致亭吴佳玲李贞姜小建赵建忠杨明霞
Owner 山东康华生物医疗科技股份有限公司
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