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Reagent for processing trace whole blood and application thereof

A reagent and trace technology, applied in the field of reagents for processing trace amounts of whole blood, can solve the problems of affecting the test results, destroying red blood cells, not protecting other proteins and chemical components, etc. Effect

Active Publication Date: 2013-09-25
BEIJING BOHUI INNOVATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The above-mentioned invention patents are all blood cell diluents, which are used to protect cell morphology, including cell structure, but do not protect other proteins and chemical components in the blood. Some inventions even destroy some red blood cells, which affects the detection results during the detection process. , this is not the problem that the present invention finally intends to solve

Method used

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  • Reagent for processing trace whole blood and application thereof
  • Reagent for processing trace whole blood and application thereof
  • Reagent for processing trace whole blood and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: A kind of reagent for processing trace whole blood

[0047] 1. Composition (100ml):

[0048]

[0049] 2. Preparation method: Take a 500ml beaker, add 80ml of ultra-pure water, weigh each component according to the proportion, add it to the beaker, dissolve it, and adjust the pH value to 7.4 with sodium hydroxide or hydrochloric acid, and constant volume to 100ml, that is.

Embodiment 2

[0050] Embodiment 2: A kind of reagent for processing trace whole blood

[0051] 1. Composition (100ml)

[0052]

[0053] 2. Preparation method: Take a 500ml beaker, add 80ml of ultra-pure water, weigh each component according to the proportion, add it to the beaker, and adjust the pH value: 7.4 (comparative example 1 and comparative example 2), 6.8 (implementation Example 2-1), 7.0 (Example 2-2), 7.2 (Example 2-3), 7.6 (Example 2-4), then dilute to 100ml with water.

Embodiment 3

[0054] Embodiment 3: A kind of reagent for processing trace whole blood

[0055] 1. Composition (100ml):

[0056]

[0057] 2. Preparation method: Same as in Example 2, adjust the pH value respectively: 7.2 (Example 3-1), 7.3 (Example 3-2), 7.4 (Example 3-3), 7.5 (Example 3-4 ).

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Abstract

The invention relates to a reagent for processing trace whole blood and an application thereof. The reagent comprises the following components: a buffer solution, an osmotic pressure retaining agent, a protective agent, an anticoagulant and a preservative. By adopting the reagent, a trace whole blood sample can be diluted by optional times (2-1000 times) and treated to obtain dilution of serum or plasma without damaging the sample and causing erythrocyte hematolysis.

Description

technical field [0001] The invention relates to a whole blood reagent, in particular to a reagent for processing a small amount of whole blood and its application. Background technique [0002] At present, clinically, most of the detection items of blood samples (such as the detection of 25-hydroxyvitamin D3, the detection of two-and-a-half hepatitis B, etc.) use serum or plasma samples, and the way to obtain serum samples is to collect veins first. For blood, centrifuge after taking the blood, and absorb the supernatant for detection; the method of obtaining plasma samples is to collect venous blood with a blood collection tube containing an anticoagulant, then centrifuge after taking the blood, and absorb the supernatant for detection. However, the existing preparation methods all collect venous blood, then centrifuge the whole blood to separate serum and plasma, so the amount of blood needed is relatively large. [0003] There may be some special groups among the people ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/38
CPCG01N33/50
Inventor 杨奇
Owner BEIJING BOHUI INNOVATION TECH
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