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Coxsackievirus A6 and A10 IgM antibody joint detection kit and preparation method thereof

A Coxsackie virus and detection kit technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of difficult popularization, high price, complicated operation, etc., and achieve cost saving, strong adsorption ability, and elimination The effect of false positive interference

Pending Publication Date: 2019-06-07
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although polymerase chain reaction (PCR) detection is fast, specific and sensitive, its high sensitivity also makes the PCR method relatively demanding on the experimental environment and equipment, and there are problems such as high technical requirements, complicated operation, and difficulty in popularization , it is difficult for general grassroots laboratories to carry out, and the price is relatively high

Method used

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  • Coxsackievirus A6 and A10 IgM antibody joint detection kit and preparation method thereof
  • Coxsackievirus A6 and A10 IgM antibody joint detection kit and preparation method thereof
  • Coxsackievirus A6 and A10 IgM antibody joint detection kit and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0071] Embodiment one: as attached figure 1 - attached Figure 7 As shown, a detection kit for Coxsackievirus A6 and A10 type IgM antibodies includes a box body 1, and a first groove 11 is provided in the box body 1, and a detection card 2 is placed in the first groove 11 , the box body 1 is provided with a second groove 12, the second groove 12 places the bottle body 3 of the sample diluent, and the first groove 11 is provided with a take-out groove 13; The setting can facilitate the taking out of the detection card 2 .

[0072] The test card 2 includes a card cover 21, a card body 22 clamped with the card cover 21, and a replaceable test strip 4 between the card cover 21 and the card body 22; the card The cover 21 is provided with a diluent sampling hole 211, a sample sampling hole 212, an observation hole 213 and an installation mark hole 214 in sequence, and the card cover 21 is provided with a first clamping device that is clamped with the card body 22 215; the card bo...

Embodiment 2

[0098] A kind of preparation method of the detection kit of Coxsackie virus A6, A10 type IgM antibody comprises the following steps:

[0099] 1. Preparation of sample diluent

[0100] Dilute 5mg / mL casein, 20mg / mL sucrose, 8.775µg / mL sodium chloride and 1mg / mL sodium azide to 100mL with 20mM buffer, and adjust the pH to 7.4.

[0101] 2. Preparation of sample pad

[0102] (1) Preparation of sample pad pretreatment solution

[0103] Dilute 0.1mg / mL rabbit anti-RBC, 10g PEG20000 and 0.5mg / mL Tween20 to 1L with 20mMPBS buffer, and adjust the pH to 7.4.

[0104] (2) Pretreatment of the second sample cushion

[0105] Soak the cut glass fiber in the sample pad pretreatment solution in the step (1), let it stand for 5 minutes, take it out, dry it and seal it for future use.

[0106] (3) Treatment of the first sample cushion

[0107] Cut the purchased blood filter membrane into strips with a width of 30 cm and a width of 0.7 cm.

[0108] 3. Preparation of latex layer

[0109] (1...

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Abstract

The invention discloses a coxsackievirus A6 and A10 IgM antibody joint detection kit and a preparation method thereof. Through the kit, a detection card and a sample diluent can be conveniently carried together; through the arrangement of a test paper clamping slot, a test strip can be quickly and precisely placed into a clamp body; through the arrangement of a clamping cover and the clamping body, the test strip can be replaced conveniently, repeated utilization can be realized, cost is saved, and the requirement of environmental protection is met; and through the arrangement of an adjustmentplate, the test strip can be kept horizontal in the clamping body, and better climbing and chromatography of whole blood are benefited.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis. [0002] Specifically, it relates to a coxsackievirus type A6 and A10 type IgM antibody combined detection kit and a preparation method thereof. Background technique [0003] Hand, foot and mouth disease (HFMD) is a common acute infectious disease among infants and young children. For children under 5 years old. Severe complications of central nervous system symptoms such as aseptic meningitis, encephalitis, and acute flaccid paralysis may occur in a small number of patients, and severe cases may lead to death. Since the outbreak of HFMD in my country in 2008, HFMD has become one of the serious public health problems in my country. As of April 2018, 16.448 million cases of HFMD and 3545 infant deaths have been reported nationwide. [0004] In recent years, the main pathogens causing HFMD in mainland China are enterovirus 71 (EV71) and coxsackievirus A16 (CoxA16). However, since 20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/531
Inventor 杨致亭黎村艳姚立琼吴佳玲李贞杨明霞
Owner 山东康华生物医疗科技股份有限公司
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