Method for determining titer of swine flu inactivated vaccine

Abstract

The invention discloses a method for determining titer of a swine flu inactivated vaccine. The method comprises the following steps: immunizing a pig with a swine flue inactivated vaccine, collecting blood 21-28 days after immunization and separating blood serum; removing non-specific components in the serum to obtain a serum to be tested; diluting the swine flue inactivated antigen, and preparing a unit swine flue antigen diluent with a concentration of 4HA; and diluting the serum to be tested by multiple proportions, successively adding the 4HA unit swine flue antigen diluent and 1% red cells to conduct hemagglutination inhibition test, so as to completely inhibit highest dilution of the 4HA unit swine flu antigen serum at HI titer. The method has advantages of greatly shortened measurement time, little operation error, strong controllability, and small inter-batch difference, and also reduces detection errors caused by different levels of experimental animals in an animal challenge protection experiment for testing the titer.

Description

technical field [0001] this invention one The invention discloses a method for testing the effectiveness of a vaccine, in particular to a method for measuring the potency of an inactivated swine influenza vaccine, belonging to the field of veterinary biological products. Background technique [0002] Swine influenza (Swine Influenza, SI) is an acute, highly contagious porcine respiratory infectious disease caused by influenza A virus of the family Orthomyxoviridae. Clinically the disease is characterized by sudden onset of cough, fever, dyspnea, prostration, rapid recovery, or death. The disease can occur in pigs of all ages and breeds, and the incidence rate can be as high as 100%. SI not only reduces the production performance of affected pigs, reduces the meat-to-feed ratio, directly affects the health of the pig herd, and causes pigs to die, but also swine influenza virus has a high affinity for pig respiratory epithelial cells, causing the respiratory tract of pigs to...

AI Technical Summary

Problems solved by technology

SI not only reduces the production performance of affected pigs, reduces the meat-to-feed ratio, directly affects the health of the pig herd, and causes pigs to die, but also swine influenza virus has a high affinity for pig respiratory epithelial cells, causing the respiratory tract of pigs to naturally The barrier is destroyed, leading to concurrent or secondary infection of various bacteria or viruses such as porcine respiratory and reproductive syndrome virus, porcine respiratory coronavirus, porcine pleuropneumoniae, etc., making the epidemic more complicated and aggravated

In addition, SI is one of the main immunosuppressive diseases of pigs, which can cause huge waste of labor, feed and medicine, resulting in huge economic losses

[0003] Inoculation with inactivated vaccine is an effective way to control swine flu. However, there is no commercialized inactivated swine flu vaccine in China at present. Determining the efficacy test method of vaccine is the key technology for vaccine development.

At present, the method used by domestic research institutes to test the efficacy of vaccines is the challenge protection test. This method takes a long time and is poor in controllability. The evaluation results are often due to the different grades of animals used, and the test results often have errors.

[0004] The inability to quickly and accurately determine the potency of swine flu vaccines has become a difficulty in the development of swine flu vaccines

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